Reviewed, UniProtKB/Swiss-Prot Q58255 (MCRG_METJA)

Names and origin

Protein names

Recommended name:
    Methyl-coenzyme M reductase I subunit gamma
      Short name=MCR I gamma
    EC=2.8.4.1
Alternative name(s):
    Coenzyme-B sulfoethylthiotransferase gamma

Gene names

Name:	mcrG
Ordered Locus Names:	MJ0845

Organism

Methanocaldococcus jannaschii (Methanococcus jannaschii) [Complete proteome] [HAMAP]

Taxonomic identifier

2190 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanocaldococcaceae › Methanocaldococcus

Protein attributes

Sequence length	260 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Reduction of methyl-coenzyme M (2-(methylthio) ethanesulfonic acid) with 7-mercaptoheptanoylthreonine phosphate to methane and an heterodisulfide By similarity.
Catalytic activity	2-(methylthio)ethanesulfonate (methyl-CoM) + N-(7-mercaptoheptanoyl)threonine 3-O-phosphate (coenzyme B) = CoM-S-S-CoB + methane.
Cofactor	Binds 2 coenzyme F430 noncovalently per hexamer. Coenzyme F430 is a yellow nickel porphinoid By similarity.
Pathway	One-carbon metabolism; methyl-CoM reduction; methane from methyl-CoM: step 1/1.
Subunit structure	Hexamer of two alpha, two beta, and two gamma chains By similarity.

Ontologies

Keywords
Biological process	Methanogenesis
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methanogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	coenzyme-B sulfoethylthiotransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 260	260	Methyl-coenzyme M reductase I subunit gamma		PRO_0000147476

Sequences

	Sequence		Length	Mass (Da)
	Q58255-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 34413840D8EADACE	FASTA	260	30,174
	10 20 30 40 50 60 MAYKPQFYPG QTKIAQNRRD HMNPDVQLEK LRDIPDDDVV KIMGHRQPGE DYKTVHPPLE 70 80 90 100 110 120 EMDLPEDYVR DLVEPLNGAK EGHRIRYIQF TDSMYFAPAQ PYDRARTYMW RFRGVDTGTL 130 140 150 160 170 180 SGRQVIEMRE SDLEALSKNF LIDTAFFDPA RIGIRGATVH GHSLRLDENG LMFDALQRYV 190 200 210 220 230 240 YDEKTGHVLY VKDQVGRPLD EPVDVGEPLP EEKLKEITTI YRIDGVPMRE DEELLTVVKR 250 260 IHRARTLGGF LPVEDVFEKL

References

[1]

"Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii." Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G., Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R., Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R., Kirkness E.F., Weinstock K.G. , Merrick J.M., Glodek A., Scott J.L., Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R., Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D., Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Science 273:1058-1073(1996) [PubMed: 8688087] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L77117 Genomic DNA. Translation: AAB98850.1.
PIR	E64405.
RefSeq	NP_247839.1.
3D structure databases
HSSP	HSSP built from PDB template 1E6V based on UniProtKB Q49604.
ModBase	Search...
Genome annotation databases
GeneID	1451733.
GenomeReviews	Gene locus MJ0845 in contig L77117_GR.
KEGG	mja:MJ0845.
NMPDR	fig|243232.1.peg.876.
TIGR	MJ0845.
Phylogenomic databases
HOGENOM	Q58255.
OMA	Q58255. GAKEGHR.
Enzyme and pathway databases
BioCyc	MJAN243232:MJ_0845-MON.
BRENDA	2.8.4.1. 256362.
Family and domain databases
InterPro	IPR003178. Me_CoM_Rdtase_gsu. [Graphical view]
Gene3D	G3DSA:3.90.320.20. MCR_gamma. 1 hit.
Pfam	PF02240. MCR_gamma. 1 hit. [Graphical view]
PIRSF	PIRSF000264. Meth_CoM_rd_gama. 1 hit.
ProDom	PD005845. MCR_gamma. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR03259. met_CoM_red_gam. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MCRG_METJA

Accession

Primary (citable) accession number: Q58255

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	June 16, 2009
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)