Q58242 (Y832_METJA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Uncharacterized protein MJ0832

Cleaved into the following 2 chains:

1. Mja rnr-1 intein
2. Mja rnr-2 intein

Gene names

Ordered Locus Names:

MJ0832

Organism

Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)

Taxonomic identifier

243232 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanocaldococcaceae › Methanocaldococcus

Protein attributes

Sequence length	1750 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Predicted

General annotation (Comments)

Post-translational modification	This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation Potential.
Sequence similarities	Contains 1 ATP-cone domain. Contains 2 DOD-type homing endonuclease domains.

Ontologies

Keywords
Domain	Repeat
Ligand	ATP-binding Nucleotide-binding
PTM	Autocatalytic cleavage Protein splicing
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	intein-mediated protein splicing Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA binding Inferred from electronic annotation. Source: InterPro endonuclease activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 337	337	Uncharacterized protein MJ0832, 1st part Potential		PRO_0000013999
	Chain	338 – 790	453	Mja rnr-1 intein Potential		PRO_0000014000
	Chain	791 – 1058	268	Uncharacterized protein MJ0832, 2nd part Potential		PRO_0000014001
	Chain	1059 – 1591	533	Mja rnr-2 intein Potential		PRO_0000014002
	Chain	1592 – 1750	159	Uncharacterized protein MJ0832, 3rd part Potential		PRO_0000014003
Regions
	Domain	14 – 101	88	ATP-cone
	Domain	507 – 634	128	DOD-type homing endonuclease 1
	Domain	1286 – 1426	141	DOD-type homing endonuclease 2

Sequences

	Sequence		Length	Mass (Da)
	Q58242 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 6D6893B770A25359	FASTA	1,750	203,281
	10 20 30 40 50 60 MISAKDFAEK VMEFYVIKRD KRKEKFNVNK LAKSLINSGV NYGDLDTIIS EVCAKVYNGI 70 80 90 100 110 120 TTDELKDIVY NVLKKIDKDV AENYRNGIIL KVRTSEKEFE SFDKEKIAKA LIRETGADEE 130 140 150 160 170 180 TARKIADEVE RELKKLKVKY LTAPMIREIV NYKLIEYGFE ELRHKHTRLG MPVYDITKLI 190 200 210 220 230 240 KSGSRENANL MYNPESIHKW VADETMKQYA LLAIFPKHIA DAHIKGDIHL HDLEYAATRP 250 260 270 280 290 300 VCLQHDLRPF FKYGLKVDGT GLHTSVSKPA KHPEVAIQHA AKVMMAAQTN MSGGQSIDEF 310 320 330 340 350 360 NVWLAPYVRG LSYEKIKQLM QMFIYELNQM YVARGGQSLG RDELIFIKEG DKLKVCKIGE 370 380 390 400 410 420 AIDEFMEKYK DKIIVDGDTE ILYLDGIAEV YTISVNVKTG KAEFKRVYAI SRHKPRGKVY 430 440 450 460 470 480 KVIGKDGTSI IVTEDHSLFN YDENGNLVCV KPRQMKHIIR NFNNPYDVEY RIGDYIETNY 490 500 510 520 530 540 QRTDSKYNSR QNDIPEKLKI TKELCQFLGL FVAEGSYITN GISITTKDDD IAKFIERFVK 550 560 570 580 590 600 EQINENIAVK RYEDSVRFVN KGFYRFLKEH INGKAINKNS PEFILKGDKE MKLAFLGGLI 610 620 630 640 650 660 SGDGYVSKDG RVQIYTTSEQ LLGQLHLLLS DLGMIYSITK IKEEGEKIEI KRNEIVRNYK 670 680 690 700 710 720 LYVIEIAKNC TEDLKPYVIP KYKKERIKPA NYDQLPYDYR IIKEHLRKIT DKKPYNDYAW 730 740 750 760 770 780 KSNNRKLKLN TLEKIEQLNP HLREEINKFK LNIPFEIKEI KEIDYNGYVY DLSVEDNENF 790 800 810 820 830 840 ITATGILCHN TIFSSINLEL EIPEFLKDKP AVIAGTTRGT YGDYEEEAKL ILEALVDVMM 850 860 870 880 890 900 EGDAMGKPFL FPNFIIKLRE NAFKDENKEL MYKIHQLSAK FGIPYFINML PDWQVTNTNA 910 920 930 940 950 960 MGCRTRLSGN WTGDAEIDTL RTGNMQWYSL NLPRIAYEAN GDDTKLFEIL HERLEILKEA 970 980 990 1000 1010 1020 LLIKHEVTKE RLYVDNLMPF LTQEFDGESY YRYENTTKTF GFVGLNEMLK YHLGEELHES 1030 1040 1050 1060 1070 1080 KDAVKFGEKV IEYIREYADK LKEETGLRWT VTQTPAESSL PYDEKILIFE NNEYKLVKIG 1090 1100 1110 1120 1130 1140 EFVEKYLNRY KDRAITYGDN NIEVYIKDEN IYAPSFDKDG KIVLKPITHA IRHRGKEIYE 1150 1160 1170 1180 1190 1200 IELESGKKVR VTGDHSVFTI NDNLDVVEVK ASDLKVGDFI ITPKIIPSIS KDKIYLSEIV 1210 1220 1230 1240 1250 1260 KNKDKYYVKI KDHIKFIEEH EEILKESYKE YKTKWKDLKP VLKKKNAFRL DLIEDLVDKE 1270 1280 1290 1300 1310 1320 KIEKISYGHA NYINNKIKLD EKFGYLIGAF LSEGHWNDKC VEISSTNKEF IENLVEIIEE 1330 1340 1350 1360 1370 1380 ILGKDAYYIT VKGDKRRYKD LYVIGLNKTV AMIFESLGLN KLSSNKEIPS ILLSNETFLK 1390 1400 1410 1420 1430 1440 GLIKGYIDGD GSIYVDESKR DYSIRLYTTS ETLRDTLCLA LKILGINYRL SIDKKSKVNE 1450 1460 1470 1480 1490 1500 NWRDCYVIKI TGKENIEKLL DVEIKNNGGK DVIPKIAEKF KEIINQYSQR EWKERFGIDV 1510 1520 1530 1540 1550 1560 NNLHIWEDLK KGYMSRYRAK KVLNIMKNVK EIEEKYGRLL DKIGQLIDND LLFERIKSIR 1570 1580 1590 1600 1610 1620 VLDEIPEYVY DISVEGTENF IGGEGFICLH NTAGRFARLD YKYYKEETIS VVRGDLNDVD 1630 1640 1650 1660 1670 1680 SLYYTNSSHV RVDAPITLGE KVRIEEKFHP LCNGGHIMHI WNIESAADPE VLMDITKKIT 1690 1700 1710 1720 1730 1740 KTHIGFWTYT KNLSVCNRCG ISMGGLRDRC INCGSEDVAK FSRITGYLQN ISNWNRAKQK 1750 ELEDRKLPRI

References

[1]

"Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii." Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G., Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R., Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R., Kirkness E.F., Weinstock K.G. , Merrick J.M., Glodek A., Scott J.L., Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R., Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D., Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Science 273:1058-1073(1996) [PubMed: 8688087] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L77117 Genomic DNA. Translation: AAB98834.1.
PIR	H64403.
RefSeq	NP_247823.1. NC_000909.1.
3D structure databases
ProteinModelPortal	Q58242.
ModBase	Search...
Protein family/group databases
MEROPS	N10.007.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1451715.
GenomeReviews	Gene locus MJ0832 in contig L77117_GR.
KEGG	mja:MJ_0832.
NMPDR	fig|243232.1.peg.860.
TIGR	MJ0832.
Phylogenomic databases
HOGENOM	HBG540930.
OMA	RQNDIPE.
ProtClustDB	CLSK796603.
Enzyme and pathway databases
BioCyc	MJAN243232:MJ_0832-MONOMER.
Family and domain databases
InterPro	IPR005144. ATP-cone. IPR003586. Hedgehog_hint_C. IPR003587. Hedgehog_hint_N. IPR007869. Homing_endonuc. IPR006142. INTEIN. IPR004042. Intein_endonuc. IPR006141. Intein_splice_site. [Graphical view]
Pfam	PF03477. ATP-cone. 2 hits. PF05204. Hom_end. 1 hit. [Graphical view]
PRINTS	PR00379. INTEIN.
SMART	SM00305. HintC. 2 hits. SM00306. HintN. 2 hits. [Graphical view]
TIGRFAMs	TIGR01443. Intein_Cterm. 2 hits. TIGR01445. Intein_Nterm. 2 hits.
PROSITE	PS51161. ATP_CONE. 1 hit. PS50818. INTEIN_C_TER. 2 hits. PS50819. INTEIN_ENDONUCLEASE. 2 hits. PS50817. INTEIN_N_TER. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

Y832_METJA

Accession

Primary (citable) accession number: Q58242

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	October 19, 2011
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program