Reviewed, UniProtKB/Swiss-Prot Q58192 (TF2B_METJA)

Names and origin

Protein names

Recommended name:
    Transcription initiation factor IIB
      Short name=TFIIB
Cleaved into the following chain:
    1- Recommended name:
            Endonuclease Mja Tfb
              EC=3.1.-.-
        Alternative name(s):
            Mja Tfb intein
            Mja TFIIB intein

Gene names

Name:	tfb
Ordered Locus Names:	MJ0782

Organism

Methanocaldococcus jannaschii (Methanococcus jannaschii) [Complete proteome] [HAMAP]

Taxonomic identifier

2190 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanocaldococcaceae › Methanocaldococcus

Protein attributes

Sequence length	673 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Stabilizes TBP binding to an archaeal box-A promoter. Also responsible for recruiting RNA polymerase II to the pre-initiation complex (DNA-TBP-TFIIB). HAMAP MF_00383
Cofactor	Binds 1 zinc ion per subunit By similarity.
Post-translational modification	This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation Potential.
Sequence similarities	Belongs to the TFIIB family. Contains 1 DOD-type homing endonuclease domain. Contains 1 TFIIB-type zinc finger.

Ontologies

Keywords
Biological process	Intron homing Transcription Transcription regulation
Domain	Repeat Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Endonuclease Hydrolase Nuclease
PTM	Autocatalytic cleavage Protein splicing
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	intein-mediated protein splicing Inferred from electronic annotation. Source: InterPro intron homing Inferred from electronic annotation. Source: UniProtKB-KW regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: HAMAP transcription initiation Inferred from electronic annotation. Source: HAMAP translational initiation Inferred from electronic annotation. Source: InterPro
Molecular function	endonuclease activity Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from electronic annotation. Source: InterPro transcription factor activity Inferred from electronic annotation. Source: HAMAP translation initiation factor activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 99	99	Transcription initiation factor IIB, 1st part Potential		PRO_0000033642
	Chain	100 – 434	335	Endonuclease Mja Tfb Potential		PRO_0000033643
	Chain	435 – 673	239	Transcription initiation factor IIB, 2nd part Potential		PRO_0000033644
Regions
	Domain	238 – 357	120	DOD-type homing endonuclease
	Repeat	490 – 573	84	1 HAMAP MF_00383
	Repeat	584 – 665	82	2 HAMAP MF_00383
	Zinc finger	38 – 69	32	TFIIB-type HAMAP MF_00383
Sites
	Metal binding	42	1	Zinc By similarity
	Metal binding	45	1	Zinc By similarity
	Metal binding	61	1	Zinc By similarity
	Metal binding	64	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q58192-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 20EA4713EAB031A9	FASTA	673	76,969
	10 20 30 40 50 60 MVWLMEALKT KENETTKEKK LTTKVEKSEK KEENVREEEI VCPICGSKEV VKDYERAEIV 70 80 90 100 110 120 CAKCGCVIKE KLFDIGPEWR AFDHEQKIKR CRVGAPMTYS VDYNEPIIIK ENGEIKVVKI 130 140 150 160 170 180 GELIDKIIEN SENIRREGIL EIAKCKGIEV IAFNSNYKFK FMPVSEVSRH PVSEMFEIVV 190 200 210 220 230 240 EGNKKVRVTR SHSVFTIRDN EVVPIRVDEL KVGDILVLAK ELPNIEEDIE IDKKFSKILG 250 260 270 280 290 300 YIIAEGYYDD KKIVLSYDYN EKEFINETID YFKSLNSDIT IYSKDLNIQI EVKNKKIINL 310 320 330 340 350 360 LKKLRVKNKR IPSIIFKSPY EIKKSFIDGI FNGKDAKVFV SKELAEDVIF LLLQIKENAT 370 380 390 400 410 420 INKKSINDIE VYEVRRITNI YTNRKLEKLI NSDFIFLKIK EINKVEPTSG YAYDLTVPNA 430 440 450 460 470 480 ENFVAGFGGF VLHNTIHDKG LSTVIDWRNK DSYGKDLSAN KRAQLYRLRK WQRRIRVSDA 490 500 510 520 530 540 AERNLAFALS ELDRITSKLG LPRHVRENAA IIYRGAVEKG LIRGRSIEGV VAAAIYAACR 550 560 570 580 590 600 RCRVPRTLDE IAEASRVDRK EIGRTYRFLA RELNIKLTPT NPIDYVPRFA SELGLPGEVE 610 620 630 640 650 660 SKAIQILQQA AEKGLTSGRG PTGVAAAAIY IASVLLGCRR TQREVAEVAG VTEVTIRNRY 670 KELTEHLDID VTL

References

[1]

"Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii." Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G., Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R., Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R., Kirkness E.F., Weinstock K.G. , Merrick J.M., Glodek A., Scott J.L., Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R., Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D., Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Science 273:1058-1073(1996) [PubMed: 8688087] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L77117 Genomic DNA. Translation: AAB98771.1.
PIR	F64397.
RefSeq	NP_247767.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1451659.
GenomeReviews	Gene locus MJ0782 in contig L77117_GR.
KEGG	mja:MJ0782.
NMPDR	fig|243232.1.peg.804.
TIGR	MJ0782.
Family and domain databases
HAMAP	MF_00383. [Tree]
InterPro	IPR006670. Cyclin. IPR011028. Cyclin-like. IPR013763. Cyclin_related. IPR003586. Hedgehog_hint_C. IPR003587. Hedgehog_hint_N. IPR006142. INTEIN. IPR004042. Intein_endonuc. IPR006141. Intein_splicing_site. IPR000812. TFIIB-related. IPR013150. TFIIB_cyclin. IPR013137. Znf_TFIIB. [Graphical view]
Gene3D	G3DSA:1.10.472.10. Cyclin_related. 2 hits.
PANTHER	PTHR11618. TFIIB_euk_relate. 1 hit.
Pfam	PF00382. TFIIB. 2 hits. PF08271. TFIIB_Zn_Ribbon. 1 hit. [Graphical view]
PRINTS	PR00379. INTEIN. PR00685. TIFACTORIIB.
SMART	SM00385. CYCLIN. 2 hits. SM00305. HintC. 1 hit. SM00306. HintN. 1 hit. [Graphical view]
TIGRFAMs	TIGR01443. intein_Cterm. 1 hit. TIGR01445. intein_Nterm. 1 hit.
PROSITE	PS50818. INTEIN_C_TER. 1 hit. PS50819. INTEIN_ENDONUCLEASE. 1 hit. PS50817. INTEIN_N_TER. 1 hit. PS00782. TFIIB. 2 hits. PS51134. ZF_TFIIB. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TF2B_METJA

Accession

Primary (citable) accession number: Q58192

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	November 24, 2009
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)