Reviewed, UniProtKB/Swiss-Prot Q58138 (COOS_METJA)

Names and origin

Protein names

Recommended name:
    Carbon monoxide dehydrogenase
      Short name=CODH
    EC=1.2.99.2

Gene names

Name:	cooS
Ordered Locus Names:	MJ0728

Organism

Methanocaldococcus jannaschii (Methanococcus jannaschii) [Complete proteome] [HAMAP]

Taxonomic identifier

2190 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanocaldococcaceae › Methanocaldococcus

Protein attributes

Sequence length	624 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	CO + H(2)O + A = CO(2) + AH(2).
Cofactor	Binds 3 4Fe-4S clusters per homodimer By similarity. Binds 2 nickel-iron-sulfur clusters per homodimer By similarity.
Subunit structure	Homodimer By similarity.
Domain	Cluster B is an all-cysteinyl-liganded 4Fe4S cluster; cluster C is a mixed Ni-Fe-S cluster which appears to be the active site of CO oxidation. Cluster D is also an all-cysteinyl-liganded 4Fe4S cluster that bridges the two subunits of the CODH dimer By similarity.
Sequence similarities	Belongs to the Ni-containing carbon monoxide dehydrogenase family.
Caution	This protein lacks the conserved Cys in position 45; it is replaced by a Tyr. It is therefore possible that the D-cluster is either altered or missing in this protein, which may not form homodimers.

Ontologies

Keywords
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding Nickel
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	generation of precursor metabolites and energy Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: InterPro carbon-monoxide dehydrogenase (acceptor) activity Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW nickel ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 624	624	Carbon monoxide dehydrogenase		PRO_0000157143
Sites
	Metal binding	37	1	Iron-sulfur 1 (4Fe-4S); shared with dimeric partner By similarity
	Metal binding	46	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	49	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	54	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	65	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	256	1	Nickel-iron-sulfur By similarity
	Metal binding	292	1	Nickel-iron-sulfur By similarity
	Metal binding	336	1	Nickel-iron-sulfur By similarity
	Metal binding	444	1	Nickel-iron-sulfur By similarity
	Metal binding	475	1	Nickel-iron-sulfur By similarity
	Metal binding	516	1	Nickel-iron-sulfur By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q58138-1 [UniParc]. Last modified June 1, 1998. Version 2. Checksum: FFB6AA3376D1050E	FASTA	624	67,408
	10 20 30 40 50 60 MKNCIAAIPE VKEMVEKAKL KGIETPHTRF PNQFPKCPYG LKGVYCILCA NGPCRITEKT 70 80 90 100 110 120 PYGVCGATAD VIVARNLCRA VAAGTSCYVH CAENAARALL SAGKGEGSYE IRNEKKLKFL 130 140 150 160 170 180 AKKLGFDANK DAKQLAVEVA EFILDDMYKP RWEKSELVPK LCPEKRLEVF EKLDILPGGA 190 200 210 220 230 240 KGEIVDALTK TSTNLNSNPM DLLVHCLRLG LHAGFTGLLM TCWLNDILFG SPKITVVENG 250 260 270 280 290 300 FSSVKPNNVN IMITGHQHAL IQPLCEAAME EDLIKMAKEA GADEIKIIGA TCNGQDMETR 310 320 330 340 350 360 IAHLPESFVG YIANNFTTEP LVATGLIDAV VSEFNCTFHG LKFVAEKTKT KLICIDDMAY 370 380 390 400 410 420 VEGAEYIPWE PENAKEKARE IIKKAIEAFK ERKGMQKDYY DEKVKSVVGV GEESLVEFLG 430 440 450 460 470 480 GSVKPLIELI ASGKIKGVVG VVGCSNLASG GHDNIIVTLT KELIKRDILV LAGGCVNSPL 490 500 510 520 530 540 KHAGLFDPAS AELAGENLKE VCKSLGIPPV LNFGACLSIA RIEQVAVAIA EELGVDIPDL 550 560 570 580 590 600 PVAASAPQWL EEQALADATY AVDMGFTVHV SPVPFVTGSE LVTKVLTEAV EGLTGGKLIP 610 620 EPNPYKAADL LEQTIMEKRK KLGI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii." Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G., Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R., Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R., Kirkness E.F., Weinstock K.G. , Merrick J.M., Glodek A., Scott J.L., Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R., Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D., Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Science 273:1058-1073(1996) [PubMed: 8688087] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.
[2]	"The Ni-containing carbon monoxide dehydrogenase family: light at the end of the tunnel?" Lindahl P.A. Biochemistry 41:2097-2105(2002) [PubMed: 11841199] [Abstract] Cited for: REVIEW.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L77117 Genomic DNA. Translation: AAB98724.1.
PIR	H64390.
RefSeq	NP_247713.1.
3D structure databases
HSSP	HSSP built from PDB template 1JJY based on UniProtKB Q9F8A8.
ModBase	Search...
Genome annotation databases
GeneID	1451605.
GenomeReviews	Gene locus MJ0728 in contig L77117_GR.
KEGG	mja:MJ0728.
NMPDR	fig|243232.1.peg.750.
TIGR	MJ0728.
Phylogenomic databases
HOGENOM	Q58138.
Enzyme and pathway databases
BioCyc	MJAN243232:MJ_0728-MON.
Family and domain databases
InterPro	IPR016101. CO_DHase_a-bundle. IPR010047. CO_DHase_cat. IPR004137. Prismane. IPR016099. Prismane-like_a/b-sand. [Graphical view]
Gene3D	G3DSA:1.20.1270.30. CO_DH_a-bundle. 1 hit. G3DSA:3.40.50.2030. Prismane-like_a/b-sand. 1 hit.
Pfam	PF03063. Prismane. 1 hit. [Graphical view]
PIRSF	PIRSF005023. CODH. 1 hit.
TIGRFAMs	TIGR01702. CO_DH_cata. 1 hit.
ProtoNet	Search...

Entry information

Entry name

COOS_METJA

Accession

Primary (citable) accession number: Q58138

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2003
Last sequence update:	June 1, 1998
Last modified:	November 25, 2008
This is version 50 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)