Reviewed, UniProtKB/Swiss-Prot Q58122 (G1PDH_METJA)

Names and origin

Protein names

Recommended name:
    Glycerol-1-phosphate dehydrogenase [NAD(P)+]
      Short name=G1P dehydrogenase
      Short name=G1PDH
    EC=1.1.1.261
Alternative name(s):
    sn-glycerol-1-phosphate dehydrogenase
    Enantiomeric glycerophosphate synthase

Gene names

Name:	egsA
Ordered Locus Names:	MJ0712

Organism

Methanocaldococcus jannaschii (Methanococcus jannaschii) [Complete proteome] [HAMAP]

Taxonomic identifier

2190 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanocaldococcaceae › Methanocaldococcus

Protein attributes

Sequence length	335 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the NAD(P)H-dependent reduction of dihydroxyacetonephosphate (DHAP or glycerone phosphate) to glycerol-1-phosphate (G1P). The G1P thus generated is used as the glycerophosphate backbone of phospholipids in the cellular membranes of Archaea By similarity.
Catalytic activity	sn-glycerol-1-phosphate + NAD(P)+ = glycerone phosphate + NAD(P)H. HAMAP MF_00497
Cofactor	Binds 1 zinc ion per subunit By similarity.
Pathway	Membrane lipid metabolism; glycerophospholipid metabolism. HAMAP MF_00497
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the glycerol-1-phosphate dehydrogenase family.

Ontologies

Keywords
Biological process	Phospholipid biosynthesis
Cellular component	Cytoplasm
Ligand	Metal-binding NAD NADP Zinc
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW phospholipid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-dehydroquinate synthase activity Inferred from electronic annotation. Source: InterPro glycerol-1-phosphate dehydrogenase [NAD(P)+] activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 335	335	Glycerol-1-phosphate dehydrogenase [NAD(P)+] HAMAP MF_00497		PRO_0000157342
Regions
	Nucleotide binding	78 – 82	5	NAD By similarity
	Nucleotide binding	100 – 103	4	NAD By similarity
Sites
	Metal binding	148	1	Zinc; catalytic By similarity
	Metal binding	226	1	Zinc; catalytic By similarity
	Metal binding	247	1	Zinc; catalytic By similarity
	Binding site	105	1	Substrate By similarity
	Binding site	109	1	NAD By similarity
	Binding site	148	1	Substrate By similarity
	Binding site	230	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q58122-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: A99C777C3126367B	FASTA	335	37,275
	10 20 30 40 50 60 MIIVTPRYTI IEDGAINKIE EILKKLNLKN PLVITGKNTK KYCRFFYDIV YYDEILNNLE 70 80 90 100 110 120 IELKKYTAYD CVIGIGGGRS IDTGKYLAYK LGIPFISVPT TASNDGIASP IVSIRQPSFM 130 140 150 160 170 180 VDAPIAIIAD TEIIKKSPRR LLSAGMGDIV SNITAVLDWK LAYKEKGEKY SESSAIFSKT 190 200 210 220 230 240 IAKELISYVL NSDLSEYHNK LVKALVGSGI AIAIANSSRP ASGSEHLFSH ALDKLKEEYN 250 260 270 280 290 300 LNINSLHGEQ CGIGTIMMSY LHEKENKKLS GLHEKIKMSL KKVDAPTTAK ELGFDEDIII 310 320 330 EALTMAHKIR NRWTILRDGL SREEARKLAE ETGVI

References

[1]

"Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii." Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G., Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R., Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R., Kirkness E.F., Weinstock K.G. , Merrick J.M., Glodek A., Scott J.L., Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R., Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D., Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Science 273:1058-1073(1996) [PubMed: 8688087] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L77117 Genomic DNA. Translation: AAB98707.1.
PIR	H64388.
RefSeq	NP_247697.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1451590.
GenomeReviews	Gene locus MJ0712 in contig L77117_GR.
KEGG	mja:MJ0712.
NMPDR	fig|243232.1.peg.734.
TIGR	MJ0712.
Phylogenomic databases
HOGENOM	Q58122.
OMA	SESSAIF.
Enzyme and pathway databases
BioCyc	MJAN243232:MJ_0712-MON.
BRENDA	1.1.1.261. 256362.
Family and domain databases
HAMAP	MF_00497. [Tree]
InterPro	IPR002658. DHQ_synth_AroB. IPR016205. Glycerol_DH. [Graphical view]
Pfam	PF01761. DHQ_synthase. 1 hit. [Graphical view]
PIRSF	PIRSF000112. Glycerol_dehydrogenase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

G1PDH_METJA

Accession

Primary (citable) accession number: Q58122

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	November 3, 2009
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)