Reviewed, UniProtKB/Swiss-Prot Q58116 (HMDH_METJA)

Names and origin

Protein names

Recommended name:
    3-hydroxy-3-methylglutaryl-coenzyme A reductase
      Short name=HMG-CoA reductase
    EC=1.1.1.34

Gene names

Name:	hmgA
Ordered Locus Names:	MJ0705

Organism

Methanocaldococcus jannaschii (Methanococcus jannaschii) [Complete proteome] [HAMAP]

Taxonomic identifier

2190 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanocaldococcaceae › Methanocaldococcus

Protein attributes

Sequence length	405 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Converts HMG-CoA to mevalonate By similarity.
Catalytic activity	(R)-mevalonate + CoA + 2 NADP(+) = (S)-3-hydroxy-3-methylglutaryl-CoA + 2 NADPH.
Pathway	Metabolic intermediate biosynthesis; mevalonic acid biosynthesis; (R)-mevalonic acid from acetyl-CoA: step 3/3.
Sequence similarities	Belongs to the HMG-CoA reductase family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	coenzyme A metabolic process Inferred from electronic annotation. Source: InterPro isoprenoid biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: InterPro
Molecular function	NADP binding Inferred from electronic annotation. Source: InterPro hydroxymethylglutaryl-CoA reductase (NADPH) activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 405	405	3-hydroxy-3-methylglutaryl-coenzyme A reductase		PRO_0000114461
Sites
	Active site	100	1	Charge relay system By similarity
	Active site	310	1	Charge relay system By similarity
	Active site	400	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q58116-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: F8F8E4729A77DFC9	FASTA	405	44,128
	10 20 30 40 50 60 MENYNDILEK MLNGEIKPYQ LDKMFGSKIA TEIRRKFIEK KVGIEFKHIC NYSIDEEMAM 70 80 90 100 110 120 KKNIENMIGA IQIPLGFAGP LKINGEYAKG EFYIPLATTE GALVASVNRG CSIITKCGGA 130 140 150 160 170 180 TVRVIDDKMT RAPCLKTKSV VDAIKVRDWI RENFERIKEV AESTTRHGKL IKIEPILIVG 190 200 210 220 230 240 RNLYPRFVFK TGDAMGMNMV TIATEKACNF IEGELKKEGI FVKTVAVSGN ACVDKKPSGM 250 260 270 280 290 300 NLINGRGKSI VAEVFLTEKE VNKYLKTTSQ AIAEVNRLKN YIGSAISNSM GFNAHYANII 310 320 330 340 350 360 GAIFLATGQD EAHIVEGSLG ITMAEVEDDG LYFSVTLPDV PIGTVGGGTR VETQKECLEM 370 380 390 400 LGCYGDNKAL KFAEIVGAAV LAGELSLLGA LAAGHLGKAH QELGR

References

[1]

"Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii." Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G., Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R., Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R., Kirkness E.F., Weinstock K.G. , Merrick J.M., Glodek A., Scott J.L., Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R., Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D., Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Science 273:1058-1073(1996) [PubMed: 8688087] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L77117 Genomic DNA. Translation: AAB98699.1.
PIR	A64388.
RefSeq	NP_247690.1.
3D structure databases
HSSP	HSSP built from PDB template 1HWI based on UniProtKB P04035.
ModBase	Search...
Genome annotation databases
GeneID	1451573.
GenomeReviews	Gene locus MJ0705 in contig L77117_GR.
KEGG	mja:MJ0705.
NMPDR	fig|243232.1.peg.727.
TIGR	MJ0705.
Phylogenomic databases
HOGENOM	Q58116.
Enzyme and pathway databases
BioCyc	MJAN243232:MJ_0705-MON.
Family and domain databases
InterPro	IPR002202. HMG_CoA_Rdtase_cat. IPR004554. HMG_CoA_Rdtase_I_cat. [Graphical view]
Gene3D	G3DSA:3.90.770.10. HMG-CoA_red. 1 hit.
PANTHER	PTHR10572. HMG-CoA_red. 1 hit.
Pfam	PF00368. HMG-CoA_red. 1 hit. [Graphical view]
PRINTS	PR00071. HMGCOARDTASE.
TIGRFAMs	TIGR00533. HMG_CoA_R_NADP. 1 hit.
PROSITE	PS00066. HMG_COA_REDUCTASE_1. 1 hit. PS00318. HMG_COA_REDUCTASE_2. 1 hit. PS01192. HMG_COA_REDUCTASE_3. False negative. PS50065. HMG_COA_REDUCTASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HMDH_METJA

Accession

Primary (citable) accession number: Q58116

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	November 25, 2008
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)