Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q58108

- FLPA_METJA

UniProt

Q58108 - FLPA_METJA

Protein

Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase

Gene

flpA

Organism
Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 93 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Involved in pre-rRNA and tRNA processing. Utilizes the methyl donor S-adenosyl-L-methionine to catalyze the site-specific 2'-hydroxyl methylation of ribose moieties in rRNA and tRNA. Site specificity is provided by a guide RNA that base pairs with the substrate. Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA.UniRule annotation

    GO - Molecular functioni

    1. methyltransferase activity Source: UniProtKB-HAMAP
    2. RNA binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. rRNA processing Source: UniProtKB-HAMAP
    2. tRNA processing Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    rRNA processing, tRNA processing

    Keywords - Ligandi

    RNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Fibrillarin-like rRNA/tRNA 2'-O-methyltransferaseUniRule annotation (EC:2.1.1.-UniRule annotation)
    Gene namesi
    Name:flpAUniRule annotation
    Ordered Locus Names:MJ0697
    OrganismiMethanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
    Taxonomic identifieri243232 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaMethanococciMethanococcalesMethanocaldococcaceaeMethanocaldococcus
    ProteomesiUP000000805: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 230230Fibrillarin-like rRNA/tRNA 2'-O-methyltransferasePRO_0000148533Add
    BLAST

    Interactioni

    Subunit structurei

    Interacts with nop5. Component of box C/D small ribonucleoprotein (sRNP) particles that contain rpl7ae, FlpA and nop5, plus a guide RNA.UniRule annotation

    Protein-protein interaction databases

    IntActiQ58108. 2 interactions.
    STRINGi243232.MJ0697.

    Structurei

    Secondary structure

    1
    230
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi5 – 95
    Turni10 – 123
    Beta strandi13 – 175
    Beta strandi19 – 213
    Beta strandi25 – 284
    Beta strandi36 – 383
    Beta strandi41 – 444
    Beta strandi47 – 515
    Turni54 – 563
    Helixi58 – 647
    Beta strandi77 – 826
    Helixi87 – 959
    Turni96 – 983
    Beta strandi99 – 1068
    Helixi108 – 11710
    Turni118 – 1203
    Beta strandi124 – 1285
    Helixi134 – 1374
    Turni138 – 1403
    Beta strandi144 – 1496
    Helixi156 – 16712
    Beta strandi168 – 17912
    Helixi180 – 1823
    Beta strandi185 – 1873
    Helixi189 – 20315
    Beta strandi205 – 2128
    Turni214 – 2163
    Beta strandi220 – 2278

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1FBNX-ray1.60A1-230[»]
    1G8SX-ray1.60A1-230[»]
    ProteinModelPortaliQ58108.
    SMRiQ58108. Positions 1-230.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ58108.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni87 – 882S-adenosyl-L-methionine bindingUniRule annotation
    Regioni105 – 1062S-adenosyl-L-methionine bindingUniRule annotation
    Regioni130 – 1312S-adenosyl-L-methionine bindingUniRule annotation
    Regioni150 – 1534S-adenosyl-L-methionine bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the methyltransferase superfamily. Fibrillarin family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1889.
    KOiK04795.
    OMAiWNPNKSK.
    PhylomeDBiQ58108.

    Family and domain databases

    Gene3Di3.40.50.150. 1 hit.
    HAMAPiMF_00351. RNA_methyltransf_FlpA.
    InterProiIPR000692. Fibrillarin.
    IPR020813. Fibrillarin_CS.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view]
    PfamiPF01269. Fibrillarin. 1 hit.
    [Graphical view]
    PIRSFiPIRSF006540. Nop17p. 1 hit.
    PRINTSiPR00052. FIBRILLARIN.
    SUPFAMiSSF53335. SSF53335. 1 hit.
    PROSITEiPS00566. FIBRILLARIN. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q58108-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEDIKIKEIF ENIYEVDLGD GLKRIATKSI VKGKKVYDEK IIKIGDEEYR    50
    IWNPNKSKLA AAIIKGLKVM PIKRDSKILY LGASAGTTPS HVADIADKGI 100
    VYAIEYAPRI MRELLDACAE RENIIPILGD ANKPQEYANI VEKVDVIYED 150
    VAQPNQAEIL IKNAKWFLKK GGYGMIAIKA RSIDVTKDPK EIFKEQKEIL 200
    EAGGFKIVDE VDIEPFEKDH VMFVGIWEGK 230
    Length:230
    Mass (Da):25,966
    Last modified:November 1, 1996 - v1
    Checksum:i9ECAAD7C4C606756
    GO

    Mass spectrometryi

    Molecular mass is 25971 Da from positions 1 - 230. Determined by ESI. 1 Publication

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L77117 Genomic DNA. Translation: AAB98690.1.
    PIRiA64387.
    RefSeqiNP_247681.1. NC_000909.1.

    Genome annotation databases

    EnsemblBacteriaiAAB98690; AAB98690; MJ_0697.
    GeneIDi1451564.
    KEGGimja:MJ_0697.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L77117 Genomic DNA. Translation: AAB98690.1 .
    PIRi A64387.
    RefSeqi NP_247681.1. NC_000909.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1FBN X-ray 1.60 A 1-230 [» ]
    1G8S X-ray 1.60 A 1-230 [» ]
    ProteinModelPortali Q58108.
    SMRi Q58108. Positions 1-230.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q58108. 2 interactions.
    STRINGi 243232.MJ0697.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAB98690 ; AAB98690 ; MJ_0697 .
    GeneIDi 1451564.
    KEGGi mja:MJ_0697.

    Phylogenomic databases

    eggNOGi COG1889.
    KOi K04795.
    OMAi WNPNKSK.
    PhylomeDBi Q58108.

    Miscellaneous databases

    EvolutionaryTracei Q58108.

    Family and domain databases

    Gene3Di 3.40.50.150. 1 hit.
    HAMAPi MF_00351. RNA_methyltransf_FlpA.
    InterProi IPR000692. Fibrillarin.
    IPR020813. Fibrillarin_CS.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view ]
    Pfami PF01269. Fibrillarin. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF006540. Nop17p. 1 hit.
    PRINTSi PR00052. FIBRILLARIN.
    SUPFAMi SSF53335. SSF53335. 1 hit.
    PROSITEi PS00566. FIBRILLARIN. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.
    2. "Expression, purification and preliminary X-ray analysis of a fibrillarin homolog from Methanococcus jannaschii, a hyperthermophile."
      Wang H., Yokota H., Kim R., Kim S.-H.
      Acta Crystallogr. D 55:338-340(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS), MASS SPECTROMETRY.
      Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.
    3. "Crystal structure of a fibrillarin homologue from Methanococcus jannaschii, a hyperthermophile, at 1.6-A resolution."
      Wang H., Boisvert D., Kim K.K., Kim R., Kim S.-H.
      EMBO J. 19:317-323(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS), SUBUNIT.
      Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.

    Entry informationi

    Entry nameiFLPA_METJA
    AccessioniPrimary (citable) accession number: Q58108
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 93 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Methanococcus jannaschii
      Methanococcus jannaschii: entries and gene names
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3