Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q58108 (FLPA_METJA) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 92. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase

EC=2.1.1.-
Gene names
Name:flpA
Ordered Locus Names:MJ0697
OrganismMethanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii) [Reference proteome] [HAMAP]
Taxonomic identifier243232 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaMethanococciMethanococcalesMethanocaldococcaceaeMethanocaldococcus

Protein attributes

Sequence length230 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in pre-rRNA and tRNA processing. Utilizes the methyl donor S-adenosyl-L-methionine to catalyze the site-specific 2'-hydroxyl methylation of ribose moieties in rRNA and tRNA. Site specificity is provided by a guide RNA that base pairs with the substrate. Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA By similarity. HAMAP-Rule MF_00351

Subunit structure

Interacts with nop5. Component of box C/D small ribonucleoprotein (sRNP) particles that contain rpl7ae, FlpA and nop5, plus a guide RNA By similarity. Ref.3

Sequence similarities

Belongs to the methyltransferase superfamily. Fibrillarin family.

Mass spectrometry

Molecular mass is 25971 Da from positions 1 - 230. Determined by ESI. Ref.2

Ontologies

Keywords
   Biological processrRNA processing
tRNA processing
   LigandRNA-binding
   Molecular functionMethyltransferase
Transferase
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processrRNA processing

Inferred from electronic annotation. Source: UniProtKB-HAMAP

tRNA processing

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Molecular_functionRNA binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 230230Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase HAMAP-Rule MF_00351
PRO_0000148533

Regions

Region87 – 882S-adenosyl-L-methionine binding By similarity
Region105 – 1062S-adenosyl-L-methionine binding By similarity
Region130 – 1312S-adenosyl-L-methionine binding By similarity
Region150 – 1534S-adenosyl-L-methionine binding By similarity

Secondary structure

................................................. 230
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q58108 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 9ECAAD7C4C606756

FASTA23025,966
        10         20         30         40         50         60 
MEDIKIKEIF ENIYEVDLGD GLKRIATKSI VKGKKVYDEK IIKIGDEEYR IWNPNKSKLA 

        70         80         90        100        110        120 
AAIIKGLKVM PIKRDSKILY LGASAGTTPS HVADIADKGI VYAIEYAPRI MRELLDACAE 

       130        140        150        160        170        180 
RENIIPILGD ANKPQEYANI VEKVDVIYED VAQPNQAEIL IKNAKWFLKK GGYGMIAIKA 

       190        200        210        220        230 
RSIDVTKDPK EIFKEQKEIL EAGGFKIVDE VDIEPFEKDH VMFVGIWEGK 

« Hide

References

« Hide 'large scale' references
[1]"Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii."
Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G., Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R., Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R., Kirkness E.F., Weinstock K.G. expand/collapse author list , Merrick J.M., Glodek A., Scott J.L., Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R., Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D., Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P., Fraser C.M., Smith H.O., Woese C.R., Venter J.C.
Science 273:1058-1073(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.
[2]"Expression, purification and preliminary X-ray analysis of a fibrillarin homolog from Methanococcus jannaschii, a hyperthermophile."
Wang H., Yokota H., Kim R., Kim S.-H.
Acta Crystallogr. D 55:338-340(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS), MASS SPECTROMETRY.
Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.
[3]"Crystal structure of a fibrillarin homologue from Methanococcus jannaschii, a hyperthermophile, at 1.6-A resolution."
Wang H., Boisvert D., Kim K.K., Kim R., Kim S.-H.
EMBO J. 19:317-323(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS), SUBUNIT.
Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L77117 Genomic DNA. Translation: AAB98690.1.
PIRA64387.
RefSeqNP_247681.1. NC_000909.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1FBNX-ray1.60A1-230[»]
1G8SX-ray1.60A1-230[»]
ProteinModelPortalQ58108.
SMRQ58108. Positions 1-230.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ58108. 2 interactions.
STRING243232.MJ0697.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAB98690; AAB98690; MJ_0697.
GeneID1451564.
KEGGmja:MJ_0697.

Phylogenomic databases

eggNOGCOG1889.
KOK04795.
OMAWNPNKSK.
PhylomeDBQ58108.

Family and domain databases

Gene3D3.40.50.150. 1 hit.
HAMAPMF_00351. RNA_methyltransf_FlpA.
InterProIPR000692. Fibrillarin.
IPR020813. Fibrillarin_CS.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PfamPF01269. Fibrillarin. 1 hit.
[Graphical view]
PIRSFPIRSF006540. Nop17p. 1 hit.
PRINTSPR00052. FIBRILLARIN.
SUPFAMSSF53335. SSF53335. 1 hit.
PROSITEPS00566. FIBRILLARIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ58108.

Entry information

Entry nameFLPA_METJA
AccessionPrimary (citable) accession number: Q58108
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: July 9, 2014
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Methanococcus jannaschii

Methanococcus jannaschii: entries and gene names