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Q58108

- FLPA_METJA

UniProt

Q58108 - FLPA_METJA

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Protein

Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase

Gene

flpA

Organism
Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Involved in pre-rRNA and tRNA processing. Utilizes the methyl donor S-adenosyl-L-methionine to catalyze the site-specific 2'-hydroxyl methylation of ribose moieties in rRNA and tRNA. Site specificity is provided by a guide RNA that base pairs with the substrate. Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA.UniRule annotation

GO - Molecular functioni

  1. methyltransferase activity Source: UniProtKB-HAMAP
  2. RNA binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. rRNA processing Source: UniProtKB-HAMAP
  2. tRNA processing Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

rRNA processing, tRNA processing

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Fibrillarin-like rRNA/tRNA 2'-O-methyltransferaseUniRule annotation (EC:2.1.1.-UniRule annotation)
Gene namesi
Name:flpAUniRule annotation
Ordered Locus Names:MJ0697
OrganismiMethanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Taxonomic identifieri243232 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanococciMethanococcalesMethanocaldococcaceaeMethanocaldococcus
ProteomesiUP000000805: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 230230Fibrillarin-like rRNA/tRNA 2'-O-methyltransferasePRO_0000148533Add
BLAST

Interactioni

Subunit structurei

Interacts with nop5. Component of box C/D small ribonucleoprotein (sRNP) particles that contain rpl7ae, FlpA and nop5, plus a guide RNA.UniRule annotation

Protein-protein interaction databases

IntActiQ58108. 2 interactions.
STRINGi243232.MJ0697.

Structurei

Secondary structure

1
230
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi5 – 95
Turni10 – 123
Beta strandi13 – 175
Beta strandi19 – 213
Beta strandi25 – 284
Beta strandi36 – 383
Beta strandi41 – 444
Beta strandi47 – 515
Turni54 – 563
Helixi58 – 647
Beta strandi77 – 826
Helixi87 – 959
Turni96 – 983
Beta strandi99 – 1068
Helixi108 – 11710
Turni118 – 1203
Beta strandi124 – 1285
Helixi134 – 1374
Turni138 – 1403
Beta strandi144 – 1496
Helixi156 – 16712
Beta strandi168 – 17912
Helixi180 – 1823
Beta strandi185 – 1873
Helixi189 – 20315
Beta strandi205 – 2128
Turni214 – 2163
Beta strandi220 – 2278

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1FBNX-ray1.60A1-230[»]
1G8SX-ray1.60A1-230[»]
ProteinModelPortaliQ58108.
SMRiQ58108. Positions 1-230.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ58108.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni87 – 882S-adenosyl-L-methionine bindingUniRule annotation
Regioni105 – 1062S-adenosyl-L-methionine bindingUniRule annotation
Regioni130 – 1312S-adenosyl-L-methionine bindingUniRule annotation
Regioni150 – 1534S-adenosyl-L-methionine bindingUniRule annotation

Sequence similaritiesi

Belongs to the methyltransferase superfamily. Fibrillarin family.UniRule annotation

Phylogenomic databases

eggNOGiCOG1889.
InParanoidiQ58108.
KOiK04795.
OMAiWNPNKSK.
PhylomeDBiQ58108.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_00351. RNA_methyltransf_FlpA.
InterProiIPR000692. Fibrillarin.
IPR020813. Fibrillarin_CS.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PfamiPF01269. Fibrillarin. 1 hit.
[Graphical view]
PIRSFiPIRSF006540. Nop17p. 1 hit.
PRINTSiPR00052. FIBRILLARIN.
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS00566. FIBRILLARIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q58108-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MEDIKIKEIF ENIYEVDLGD GLKRIATKSI VKGKKVYDEK IIKIGDEEYR
60 70 80 90 100
IWNPNKSKLA AAIIKGLKVM PIKRDSKILY LGASAGTTPS HVADIADKGI
110 120 130 140 150
VYAIEYAPRI MRELLDACAE RENIIPILGD ANKPQEYANI VEKVDVIYED
160 170 180 190 200
VAQPNQAEIL IKNAKWFLKK GGYGMIAIKA RSIDVTKDPK EIFKEQKEIL
210 220 230
EAGGFKIVDE VDIEPFEKDH VMFVGIWEGK
Length:230
Mass (Da):25,966
Last modified:November 1, 1996 - v1
Checksum:i9ECAAD7C4C606756
GO

Mass spectrometryi

Molecular mass is 25971 Da from positions 1 - 230. Determined by ESI. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L77117 Genomic DNA. Translation: AAB98690.1.
PIRiA64387.
RefSeqiNP_247681.1. NC_000909.1.
WP_010870202.1. NC_000909.1.

Genome annotation databases

EnsemblBacteriaiAAB98690; AAB98690; MJ_0697.
GeneIDi1451564.
KEGGimja:MJ_0697.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L77117 Genomic DNA. Translation: AAB98690.1 .
PIRi A64387.
RefSeqi NP_247681.1. NC_000909.1.
WP_010870202.1. NC_000909.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1FBN X-ray 1.60 A 1-230 [» ]
1G8S X-ray 1.60 A 1-230 [» ]
ProteinModelPortali Q58108.
SMRi Q58108. Positions 1-230.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q58108. 2 interactions.
STRINGi 243232.MJ0697.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAB98690 ; AAB98690 ; MJ_0697 .
GeneIDi 1451564.
KEGGi mja:MJ_0697.

Phylogenomic databases

eggNOGi COG1889.
InParanoidi Q58108.
KOi K04795.
OMAi WNPNKSK.
PhylomeDBi Q58108.

Miscellaneous databases

EvolutionaryTracei Q58108.

Family and domain databases

Gene3Di 3.40.50.150. 1 hit.
HAMAPi MF_00351. RNA_methyltransf_FlpA.
InterProi IPR000692. Fibrillarin.
IPR020813. Fibrillarin_CS.
IPR029063. SAM-dependent_MTases-like.
[Graphical view ]
Pfami PF01269. Fibrillarin. 1 hit.
[Graphical view ]
PIRSFi PIRSF006540. Nop17p. 1 hit.
PRINTSi PR00052. FIBRILLARIN.
SUPFAMi SSF53335. SSF53335. 1 hit.
PROSITEi PS00566. FIBRILLARIN. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.
  2. "Expression, purification and preliminary X-ray analysis of a fibrillarin homolog from Methanococcus jannaschii, a hyperthermophile."
    Wang H., Yokota H., Kim R., Kim S.-H.
    Acta Crystallogr. D 55:338-340(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS), MASS SPECTROMETRY.
    Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.
  3. "Crystal structure of a fibrillarin homologue from Methanococcus jannaschii, a hyperthermophile, at 1.6-A resolution."
    Wang H., Boisvert D., Kim K.K., Kim R., Kim S.-H.
    EMBO J. 19:317-323(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS), SUBUNIT.
    Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.

Entry informationi

Entry nameiFLPA_METJA
AccessioniPrimary (citable) accession number: Q58108
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: October 29, 2014
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Methanococcus jannaschii
    Methanococcus jannaschii: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3