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Q58108

- FLPA_METJA

UniProt

Q58108 - FLPA_METJA

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Protein
Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase
Gene
flpA, MJ0697
Organism
Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Involved in pre-rRNA and tRNA processing. Utilizes the methyl donor S-adenosyl-L-methionine to catalyze the site-specific 2'-hydroxyl methylation of ribose moieties in rRNA and tRNA. Site specificity is provided by a guide RNA that base pairs with the substrate. Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA By similarity.UniRule annotation

GO - Molecular functioni

  1. RNA binding Source: UniProtKB-HAMAP
  2. methyltransferase activity Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

  1. rRNA processing Source: UniProtKB-HAMAP
  2. tRNA processing Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

rRNA processing, tRNA processing

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase (EC:2.1.1.-)
Gene namesi
Name:flpA
Ordered Locus Names:MJ0697
OrganismiMethanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Taxonomic identifieri243232 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanococciMethanococcalesMethanocaldococcaceaeMethanocaldococcus
ProteomesiUP000000805: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 230230Fibrillarin-like rRNA/tRNA 2'-O-methyltransferaseUniRule annotation
PRO_0000148533Add
BLAST

Interactioni

Subunit structurei

Interacts with nop5. Component of box C/D small ribonucleoprotein (sRNP) particles that contain rpl7ae, FlpA and nop5, plus a guide RNA By similarity.1 Publication

Protein-protein interaction databases

IntActiQ58108. 2 interactions.
STRINGi243232.MJ0697.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi5 – 95
Turni10 – 123
Beta strandi13 – 175
Beta strandi19 – 213
Beta strandi25 – 284
Beta strandi36 – 383
Beta strandi41 – 444
Beta strandi47 – 515
Turni54 – 563
Helixi58 – 647
Beta strandi77 – 826
Helixi87 – 959
Turni96 – 983
Beta strandi99 – 1068
Helixi108 – 11710
Turni118 – 1203
Beta strandi124 – 1285
Helixi134 – 1374
Turni138 – 1403
Beta strandi144 – 1496
Helixi156 – 16712
Beta strandi168 – 17912
Helixi180 – 1823
Beta strandi185 – 1873
Helixi189 – 20315
Beta strandi205 – 2128
Turni214 – 2163
Beta strandi220 – 2278

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1FBNX-ray1.60A1-230[»]
1G8SX-ray1.60A1-230[»]
ProteinModelPortaliQ58108.
SMRiQ58108. Positions 1-230.

Miscellaneous databases

EvolutionaryTraceiQ58108.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni87 – 882S-adenosyl-L-methionine binding By similarity
Regioni105 – 1062S-adenosyl-L-methionine binding By similarity
Regioni130 – 1312S-adenosyl-L-methionine binding By similarity
Regioni150 – 1534S-adenosyl-L-methionine binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1889.
KOiK04795.
OMAiWNPNKSK.
PhylomeDBiQ58108.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_00351. RNA_methyltransf_FlpA.
InterProiIPR000692. Fibrillarin.
IPR020813. Fibrillarin_CS.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PfamiPF01269. Fibrillarin. 1 hit.
[Graphical view]
PIRSFiPIRSF006540. Nop17p. 1 hit.
PRINTSiPR00052. FIBRILLARIN.
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS00566. FIBRILLARIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q58108-1 [UniParc]FASTAAdd to Basket

« Hide

MEDIKIKEIF ENIYEVDLGD GLKRIATKSI VKGKKVYDEK IIKIGDEEYR    50
IWNPNKSKLA AAIIKGLKVM PIKRDSKILY LGASAGTTPS HVADIADKGI 100
VYAIEYAPRI MRELLDACAE RENIIPILGD ANKPQEYANI VEKVDVIYED 150
VAQPNQAEIL IKNAKWFLKK GGYGMIAIKA RSIDVTKDPK EIFKEQKEIL 200
EAGGFKIVDE VDIEPFEKDH VMFVGIWEGK 230
Length:230
Mass (Da):25,966
Last modified:November 1, 1996 - v1
Checksum:i9ECAAD7C4C606756
GO

Mass spectrometryi

Molecular mass is 25971 Da from positions 1 - 230. Determined by ESI. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L77117 Genomic DNA. Translation: AAB98690.1.
PIRiA64387.
RefSeqiNP_247681.1. NC_000909.1.

Genome annotation databases

EnsemblBacteriaiAAB98690; AAB98690; MJ_0697.
GeneIDi1451564.
KEGGimja:MJ_0697.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L77117 Genomic DNA. Translation: AAB98690.1 .
PIRi A64387.
RefSeqi NP_247681.1. NC_000909.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1FBN X-ray 1.60 A 1-230 [» ]
1G8S X-ray 1.60 A 1-230 [» ]
ProteinModelPortali Q58108.
SMRi Q58108. Positions 1-230.
ModBasei Search...

Protein-protein interaction databases

IntActi Q58108. 2 interactions.
STRINGi 243232.MJ0697.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAB98690 ; AAB98690 ; MJ_0697 .
GeneIDi 1451564.
KEGGi mja:MJ_0697.

Phylogenomic databases

eggNOGi COG1889.
KOi K04795.
OMAi WNPNKSK.
PhylomeDBi Q58108.

Miscellaneous databases

EvolutionaryTracei Q58108.

Family and domain databases

Gene3Di 3.40.50.150. 1 hit.
HAMAPi MF_00351. RNA_methyltransf_FlpA.
InterProi IPR000692. Fibrillarin.
IPR020813. Fibrillarin_CS.
IPR029063. SAM-dependent_MTases-like.
[Graphical view ]
Pfami PF01269. Fibrillarin. 1 hit.
[Graphical view ]
PIRSFi PIRSF006540. Nop17p. 1 hit.
PRINTSi PR00052. FIBRILLARIN.
SUPFAMi SSF53335. SSF53335. 1 hit.
PROSITEi PS00566. FIBRILLARIN. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.
  2. "Expression, purification and preliminary X-ray analysis of a fibrillarin homolog from Methanococcus jannaschii, a hyperthermophile."
    Wang H., Yokota H., Kim R., Kim S.-H.
    Acta Crystallogr. D 55:338-340(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS), MASS SPECTROMETRY.
    Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.
  3. "Crystal structure of a fibrillarin homologue from Methanococcus jannaschii, a hyperthermophile, at 1.6-A resolution."
    Wang H., Boisvert D., Kim K.K., Kim R., Kim S.-H.
    EMBO J. 19:317-323(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS), SUBUNIT.
    Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.

Entry informationi

Entry nameiFLPA_METJA
AccessioniPrimary (citable) accession number: Q58108
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: July 9, 2014
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Methanococcus jannaschii
    Methanococcus jannaschii: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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