Reviewed, UniProtKB/Swiss-Prot Q58097 (AAT2_METJA)

Names and origin

Protein names

Recommended name:
    Probable aspartate aminotransferase 2
      Short name=AspAT
    EC=2.6.1.1
Alternative name(s):
    Transaminase A

Gene names

Ordered Locus Names:

MJ0684

Organism

Methanocaldococcus jannaschii (Methanococcus jannaschii) [Complete proteome] [HAMAP]

Taxonomic identifier

2190 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanocaldococcaceae › Methanocaldococcus

Protein attributes

Sequence length	370 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate.
Cofactor	Pyridoxal phosphate By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Pyridoxal phosphate
Molecular function	Aminotransferase Transferase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	1-aminocyclopropane-1-carboxylate synthase activity Inferred from electronic annotation. Source: InterPro L-aspartate:2-oxoglutarate aminotransferase activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 370

370

Probable aspartate aminotransferase 2

PRO_0000123859

Amino acid modifications

Modified residue

222

1

N6-(pyridoxal phosphate)lysine By similarity

Secondary structure

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370

Helix Strand Turn

Details...

Helix

4 – 8

5

Helix

15 – 26

12

Helix

45 – 56

12

Helix

69 – 82

14

Helix

89 – 91

3

Beta strand

92 – 97

6

Helix

98 – 109

12

Beta strand

115 – 121

7

Helix

125 – 132

8

Beta strand

136 – 140

5

Helix

144 – 150

7

Beta strand

153 – 163

11

Turn

165 – 167

3

Helix

173 – 182

10

Beta strand

184 – 189

6

Turn

191 – 194

4

Beta strand

196 – 199

4

Helix

204 – 206

3

Beta strand

212 – 220

9

Turn

221 – 225

5

Helix

227 – 229

3

Beta strand

232 – 235

4

Helix

238 – 251

14

Beta strand

253 – 255

3

Helix

257 – 263

7

Helix

264 – 267

4

Helix

269 – 295

27

Helix

317 – 328

12

Helix

335 – 338

4

Helix

340 – 342

3

Beta strand

343 – 349

7

Helix

354 – 368

15

Sequences

	Sequence		Length	Mass (Da)
	Q58097-1 [UniParc]. Last modified December 15, 1998. Version 2. Checksum: 51C2EE6D50079940	FASTA	370	42,348
	10 20 30 40 50 60 MLSKRLLNFE SFEVMDILAL AQKLESEGKK VIHLEIGEPD FNTPKPIVDE GIKSLKEGKT 70 80 90 100 110 120 HYTDSRGILE LREKISELYK DKYKADIIPD NIIITGGSSL GLFFALSSII DDGDEVLIQN 130 140 150 160 170 180 PCYPCYKNFI RFLGAKPVFC DFTVESLEEA LSDKTKAIII NSPSNPLGEV IDREIYEFAY 190 200 210 220 230 240 ENIPYIISDE IYNGLVYEGK CYSAIEFDEN LEKTILINGF SKLYAMTGWR IGYVISNDEI 250 260 270 280 290 300 IEAILKLQQN LFISAPTISQ YAALKAFEKE TEREINSMIK EFDRRRRLVL KYVKDFGWEV 310 320 330 340 350 360 NNPIGAYYVF PNIGEDGREF AYKLLKEKFV ALTPGIGFGS KGKNYIRISY ANSYENIKEG 370 LERIKEFLNK

References

[1]

"Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii." Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G., Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R., Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R., Kirkness E.F., Weinstock K.G. , Merrick J.M., Glodek A., Scott J.L., Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R., Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D., Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Science 273:1058-1073(1996) [PubMed: 8688087] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.

Cross-references

Sequence databases

EMBLGenBankDDBJ

L77117 Genomic DNA. Translation: AAB98679.1.

RefSeq

NP_247668.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2Z61	X-ray	2.20	A	1-370	[»]

ModBase

Search...

Genome annotation databases

GeneID

1451550.

GenomeReviews

Gene locus MJ0684 in contig L77117_GR.

KEGG

mja:MJ0684.

NMPDR

fig|243232.1.peg.705.

TIGR

MJ0684.

Phylogenomic databases

HOGENOM

Q58097.

OMA

EREINSM.

Enzyme and pathway databases

BioCyc

MJAN243232:MJ_0684-MON.

BRENDA

2.6.1.1. 256362.

Family and domain databases

InterPro

IPR001176. ACC_synthase.
IPR004839. Aminotransferase_I/II.
IPR004838. NHTrfase_class1_PyrdxlP-BS.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
[Graphical view]

Gene3D

G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit.

Pfam

PF00155. Aminotran_1_2. 1 hit.
[Graphical view]

PRINTS

PR00753. ACCSYNTHASE.

PROSITE

PS00105. AA_TRANSFER_CLASS_1. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

AAT2_METJA

Accession

Primary (citable) accession number: Q58097

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	December 15, 1998
Last modified:	November 3, 2009
This is version 62 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)