Reviewed, UniProtKB/Swiss-Prot Q58095 (Y682_METJA)

Names and origin

Protein names

Recommended name:
    UPF0027 protein MJ0682
Cleaved into the following chain:
    1- Recommended name:
            Mja hyp2 intein
              EC=3.1.-.-

Gene names

Ordered Locus Names:

MJ0682

Organism

Methanocaldococcus jannaschii (Methanococcus jannaschii) [Complete proteome] [HAMAP]

Taxonomic identifier

2190 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanocaldococcaceae › Methanocaldococcus

Protein attributes

Sequence length	968 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Post-translational modification	This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation Potential.
Sequence similarities	Belongs to the UPF0027 (rtcB) family. Contains 1 DOD-type homing endonuclease domain.

Ontologies

Keywords
Biological process	Intron homing
Ligand	DNA-binding
Molecular function	Endonuclease Hydrolase Nuclease
PTM	Autocatalytic cleavage Protein splicing
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	intein-mediated protein splicing Inferred from electronic annotation. Source: InterPro intron homing Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW endonuclease activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 97	97	UPF0027 protein MJ0682, 1st part Potential		PRO_0000036183
	Chain	98 – 585	488	Mja hyp2 intein Potential		PRO_0000036184
	Chain	586 – 968	383	UPF0027 protein MJ0682, 2nd part Potential		PRO_0000036185
Regions
	Domain	245 – 414	170	DOD-type homing endonuclease

Sequences

	Sequence		Length	Mass (Da)
	Q58095-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 39A2A66280466130	FASTA	968	110,206
	10 20 30 40 50 60 MKDVLKRVSD VVWELPKDYK DCMRVPGRIY LNEILLDELE PEVLEQIANV ACLPGIYKYS 70 80 90 100 110 120 IAMPDVHYGY GFAIGGVAAF DQREGVISPG GVGFDINCLT SNSKILTDDG YYIKLEKLKE 130 140 150 160 170 180 KLDLHIKIYN TEEGEKSSNI LFVSERYADE KIIRIKTESG RVLEGSKDHP VLTLNGYVPM 190 200 210 220 230 240 GMLKEGDDVI VYPYEGVEYE EPSDEIILDE DDFAEYDKQI IKYLKDRGLL PLRMDNKNIG 250 260 270 280 290 300 IIARLLGFAF GDGSIVKENG DRERLYVAFY GKRETLIKIR EDLEKLGIKA SRIYSRKREV 310 320 330 340 350 360 EIRNAYGDEY TSLCEDNSIK ITSKAFALFM HKLGMPIGKK TEQIYKIPEW IKKAPKWVKR 370 380 390 400 410 420 NFLAGLFGAD GSRAVFKNYT PLPINLTMSK SEELKENILE FLNEIKLLLA EFDIESMIYE 430 440 450 460 470 480 IKSLDGRVSY RLAIVGEESI KNFLGRINYE YSGEKKVIGL LAYEYLRRKD IAKEIRKKCI 490 500 510 520 530 540 KRAKELYKKG VTVSEMLKMD EFRNEFISKR LIERAVYENL DEDDVRISTK FPKFEEFIEK 550 560 570 580 590 600 YGVIGGFVID KIKEIEEISY DSKLYDVGIV SKEHNFIANS IVVHNCGVRL IRTNLTKEEV 610 620 630 640 650 660 QSKIKELIKT LFKNVPSGLG SKGILKFSKS VMDDVLEEGV RWAVKEGYGW KEDLEFIEEH 670 680 690 700 710 720 GCLKDADASY VSDKAKERGR VQLGSLGSGN HFLEVQYVEK VFDEEAAEIY GIEENQVVVL 730 740 750 760 770 780 VHTGSRGLGH QICTDYLRIM EKAAKNYGIK LPDRQLACAP FESEEGQSYF KAMCCGANYA 790 800 810 820 830 840 WANRQMITHW VRESFEEVFK IHAEDLEMNI VYDVAHNIAK KEEHIIDGRK VKVIVHRKGA 850 860 870 880 890 900 TRAFPPKHEA IPKEYWSVGQ PVIIPGDMGT ASYLMRGTEI AMKETFGSTA HGAGRKLSRA 910 920 930 940 950 960 KALKLWKGKE IQRRLAEMGI VAMSDSKAVM AEEAPEAYKS VDLVADTCHK AGISLKVARM RPLGVIKG

References

[1]

"Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii." Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G., Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R., Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R., Kirkness E.F., Weinstock K.G. , Merrick J.M., Glodek A., Scott J.L., Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R., Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D., Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Science 273:1058-1073(1996) [PubMed: 8688087] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L77117 Genomic DNA. Translation: AAB98677.1.
PIR	B64385.
RefSeq	NP_247666.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1451548.
GenomeReviews	Gene locus MJ0682 in contig L77117_GR.
KEGG	mja:MJ0682.
NMPDR	fig|243232.1.peg.703.
TIGR	MJ0682.
Phylogenomic databases
HOGENOM	HBG539468.
OMA	VHNCGVR.
Family and domain databases
InterPro	IPR003586. Hedgehog_hint_C. IPR003587. Hedgehog_hint_N. IPR006142. INTEIN. IPR004042. Intein_endonuc. IPR006141. Intein_splicing_site. IPR001233. UPF0027. [Graphical view]
PANTHER	PTHR11118. UPF0027. 1 hit.
Pfam	PF01139. UPF0027. 2 hits. [Graphical view]
PRINTS	PR00379. INTEIN.
SMART	SM00305. HintC. 1 hit. SM00306. HintN. 1 hit. [Graphical view]
PROSITE	PS50818. INTEIN_C_TER. 1 hit. PS50819. INTEIN_ENDONUCLEASE. 1 hit. PS50817. INTEIN_N_TER. 1 hit. PS01288. UPF0027. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

Y682_METJA

Accession

Primary (citable) accession number: Q58095

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	December 15, 2009
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)