Q58083 (H669_METJA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable ATP-dependent RNA helicase MJ0669
EC=3.6.4.13

Gene names

Ordered Locus Names:

MJ0669

Organism

Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)

Taxonomic identifier

243232 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanocaldococcaceae › Methanocaldococcus

Protein attributes

Sequence length	367 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ATP + H2O = ADP + phosphate.
Subunit structure	Homodimer.
Sequence similarities	Belongs to the DEAD box helicase family. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding RNA-binding
Molecular function	Helicase Hydrolase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATP-dependent helicase activity Inferred from electronic annotation. Source: InterPro RNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 367

367

Probable ATP-dependent RNA helicase MJ0669

PRO_0000055116

Regions

Domain

38 – 206

169

Helicase ATP-binding

Domain

213 – 367

155

Helicase C-terminal

Nucleotide binding

51 – 58

8

ATP By similarity

Motif

6 – 34

29

Q motif

Motif

154 – 157

4

DEAD box

Secondary structure

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367

Helix Strand Turn

Details...

Helix

8 – 10

3

Helix

15 – 24

10

Helix

31 – 42

12

Beta strand

45 – 50

6

Beta strand

53 – 56

4

Helix

57 – 68

12

Beta strand

71 – 74

4

Beta strand

77 – 80

4

Helix

84 – 98

15

Beta strand

105 – 108

4

Helix

114 – 122

9

Beta strand

125 – 129

5

Helix

131 – 139

9

Beta strand

150 – 155

6

Helix

156 – 160

5

Turn

161 – 164

4

Helix

165 – 173

9

Beta strand

180 – 184

5

Helix

190 – 199

10

Beta strand

202 – 207

6

Beta strand

210 – 220

11

Helix

223 – 225

3

Helix

226 – 234

9

Beta strand

241 – 244

4

Helix

248 – 260

13

Beta strand

265 – 268

4

Beta strand

270 – 272

3

Helix

274 – 285

12

Beta strand

288 – 294

7

Helix

298 – 301

4

Beta strand

308 – 314

7

Helix

319 – 325

7

Beta strand

332 – 334

3

Beta strand

337 – 342

6

Helix

347 – 357

11

Sequences

	Sequence		Length	Mass (Da)
	Q58083 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 1B9102E8DF50CB37	FASTA	367	41,920
	10 20 30 40 50 60 MEVEYMNFNE LNLSDNILNA IRNKGFEKPT DIQMKVIPLF LNDEYNIVAQ ARTGSGKTAS 70 80 90 100 110 120 FAIPLIELVN ENNGIEAIIL TPTRELAIQV ADEIESLKGN KNLKIAKIYG GKAIYPQIKA 130 140 150 160 170 180 LKNANIVVGT PGRILDHINR GTLNLKNVKY FILDEADEML NMGFIKDVEK ILNACNKDKR 190 200 210 220 230 240 ILLFSATMPR EILNLAKKYM GDYSFIKAKI NANIEQSYVE VNENERFEAL CRLLKNKEFY 250 260 270 280 290 300 GLVFCKTKRD TKELASMLRD IGFKAGAIHG DLSQSQREKV IRLFKQKKIR ILIATDVMSR 310 320 330 340 350 360 GIDVNDLNCV INYHLPQNPE SYMHRIGRTG RAGKKGKAIS IINRREYKKL RYIERAMKLK IKKLKFG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii." Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G., Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R., Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R., Kirkness E.F., Weinstock K.G. , Merrick J.M., Glodek A., Scott J.L., Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R., Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D., Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Science 273:1058-1073(1996) [PubMed: 8688087] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.
[2]	"Crystal structure of a DEAD box protein from the hyperthermophile Methanococcus jannaschii." Story R.M., Li H., Abelson J.N. Proc. Natl. Acad. Sci. U.S.A. 98:1465-1470(2001) [PubMed: 11171974] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS).
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

L77117 Genomic DNA. Translation: AAB98663.1.

PIR

E64383.

RefSeq

NP_247653.1. NC_000909.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1HV8	X-ray	3.00	A/B	1-367	[»]

ProteinModelPortal

Q58083.

SMR

Q58083. Positions 3-365.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

1451535.

GenomeReviews

Gene locus MJ0669 in contig L77117_GR.

KEGG

mja:MJ_0669.

NMPDR

fig|243232.1.peg.690.

TIGR

MJ0669.

Phylogenomic databases

HOGENOM

HBG737336.

OMA

FKAGAIH.

ProtClustDB

CLSK2517642.

Enzyme and pathway databases

BioCyc

MJAN243232:MJ_0669-MONOMER.

Family and domain databases

InterPro

IPR014001. DEAD-like_helicase.
IPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
IPR001650. Helicase_C.
IPR000629. RNA-helicase_DEAD-box_CS.
IPR014014. RNA_helicase_DEAD_Q_motif.
[Graphical view]

Pfam

PF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
[Graphical view]

SMART

SM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]

PROSITE

PS00039. DEAD_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51195. Q_MOTIF. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

H669_METJA

Accession

Primary (citable) accession number: Q58083

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	October 19, 2011
This is version 80 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program