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Q58050 (SYL_METJA) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 90. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:MJ0633
OrganismMethanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii) [Reference proteome] [HAMAP]
Taxonomic identifier243232 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaMethanococciMethanococcalesMethanocaldococcaceaeMethanocaldococcus

Protein attributes

Sequence length942 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 942942Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_0000152131

Regions

Motif41 – 5111"HIGH" region HAMAP-Rule MF_00049
Motif633 – 6375"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6361ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q58050 [UniParc].

Last modified December 15, 1998. Version 2.
Checksum: 25A551461566A399

FASTA942110,184
        10         20         30         40         50         60 
MMVMIDFKEI EKKWQKRWEE AKIFEANPDD REKFFITAAF PYLNGVLHAG HLRTFTIPEV 

        70         80         90        100        110        120 
VARFQRMKNK NVLWTFGYHV TGTPILGLAE LIKNRDEKTI WAYTELHGIP KEELLELTTP 

       130        140        150        160        170        180 
EKIVEYFSKK AEEAFKRMGF SLDWRRNFKT DDKVFNKFIE WQFHKLKEKG LIVKGSHPVR 

       190        200        210        220        230        240 
YCPRCDNPVE DHDILVGENA TLVEYILIKF TTEDGCIMPM ATLRPETVFG VTNVWVNPEA 

       250        260        270        280        290        300 
TYVKAKVYLE KETENGIELI ENGIWIMAKE CAEKLKHQDR KIEIIEEFKG EQLINKKVKN 

       310        320        330        340        350        360 
PVTGKEVPIL PAKFVKTNIG TGCVMSVPAH APYDYIALRD LGLVDEIGLI PLINVPGYGK 

       370        380        390        400        410        420 
YPAKEIVEKM GIKSQEEEDK LEEATKKIYK DEFHKGVLNE NCLDYEGIPV REIKDKLTKD 

       430        440        450        460        470        480 
LIDKGLAEIM YEFSEEKVIC RCGTPCIVKM VKGQWFIKYS DEKWKELAHK CIDKMRFIPE 

       490        500        510        520        530        540 
NLRQVFHEKI DWMKDKACVR RRGLGTKFPF EEGWVIESLS DSTIYPAYYT VAKYINQHNI 

       550        560        570        580        590        600 
KPEQLTLELF DYVFLGKGDV DKIAKETGIP KDIIEGMRKE FIYYYPVDWR CSAKDLIPNH 

       610        620        630        640        650        660 
LTFYIFNHVA IFPEEFWPRG IVVNGYVTIE GKKLSKSKGP VLPVLEVAEK FGADVGRFYI 

       670        680        690        700        710        720 
TTCAELPQDA DIKFKEMENT KKVLERLYLF AKEIAERRGE TGEEFSYIDK WLLSRLYKAV 

       730        740        750        760        770        780 
KQYDEYMENF ELRKAGILLY QLLDDLKWYR RRGGNNIRVL EEFLEVIIKL MMPFTPHLCE 

       790        800        810        820        830        840 
EMWEILGKEG FVSLAKFPEV KEEFINDEIE KGEEYLKAVM EDIKEIINVA KVQPKRIYLY 

       850        860        870        880        890        900 
TADDWKYEIL KIIKENEGKT IKELMPIIMK NPEFRKYGKE IPKLVNQLIK LNAEIINEVE 

       910        920        930        940 
VLENAKEFLK KEVGVEDIII NGEDKANKKR VAIPFKPAIY LE 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L77117 Genomic DNA. Translation: AAB98628.1.
PIRA64379.
RefSeqNP_247617.1. NC_000909.1.

3D structure databases

ProteinModelPortalQ58050.
SMRQ58050. Positions 5-787.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING243232.MJ0633.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAB98628; AAB98628; MJ_0633.
GeneID1451499.
KEGGmja:MJ_0633.

Phylogenomic databases

eggNOGCOG0495.
KOK01869.
OMAHAPFDYL.
PhylomeDBQ58050.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_A. Leu_tRNA_synth_A.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR020791. Leu-tRNA-lgase_arc.
IPR004493. Leu-tRNA-synth_Ia_arc/euk.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 2 hits.
TIGRFAMsTIGR00395. leuS_arch. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_METJA
AccessionPrimary (citable) accession number: Q58050
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: December 15, 1998
Last modified: July 9, 2014
This is version 90 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Methanococcus jannaschii

Methanococcus jannaschii: entries and gene names

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries