Q58033 (PURE_METJA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
N5-carboxyaminoimidazole ribonucleotide mutase
Short name=N5-CAIR mutase
EC=5.4.99.18

Alternative name(s):
5-(carboxyamino)imidazole ribonucleotide mutase

Gene names

Name:	purE
Ordered Locus Names:	MJ0616

Organism

Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)

Taxonomic identifier

243232 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanocaldococcaceae › Methanocaldococcus

Protein attributes

Sequence length	157 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the conversion of N5-carboxyaminoimidazole ribonucleotide (N5-CAIR) to 4-carboxy-5-aminoimidazole ribonucleotide (CAIR) By similarity.
Catalytic activity	5-carboxyamino-1-(5-phospho-D-ribosyl)imidazole = 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate.
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate from 5-amino-1-(5-phospho-D-ribosyl)imidazole (N5-CAIR route): step 2/2.
Sequence similarities	Belongs to the AIR carboxylase family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Molecular function	Isomerase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	5-(carboxyamino)imidazole ribonucleotide mutase activity Inferred from electronic annotation. Source: EC phosphoribosylaminoimidazole carboxylase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 157

157

N5-carboxyaminoimidazole ribonucleotide mutase

PRO_0000074987

Sites

Binding site

8

1

Substrate By similarity

Binding site

11

1

Substrate By similarity

Binding site

38

1

Substrate By similarity

Secondary structure

1

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157

Helix Strand Turn

Details...

Beta strand

2 – 8

7

Helix

9 – 11

3

Helix

12 – 24

13

Beta strand

29 – 33

5

Turn

36 – 38

3

Helix

40 – 49

10

Beta strand

53 – 62

10

Helix

65 – 70

6

Beta strand

77 – 82

6

Helix

86 – 88

3

Helix

89 – 96

8

Helix

112 – 123

12

Turn

124 – 126

3

Helix

128 – 153

26

Sequences

	Sequence		Length	Mass (Da)
	Q58033 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 398E2DDA35030078	FASTA	157	16,951
	10 20 30 40 50 60 MICIIMGSES DLKIAEKAVN ILKEFGVEFE VRVASAHRTP ELVEEIVKNS KADVFIAIAG 70 80 90 100 110 120 LAAHLPGVVA SLTTKPVIAV PVDAKLDGLD ALLSSVQMPP GIPVATVGID RGENAAILAL 130 140 150 EILALKDENI AKKLIEYREK MKKKVYASDE KVKEMFK

References

[1]

"Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii." Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G., Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R., Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R., Kirkness E.F., Weinstock K.G. , Merrick J.M., Glodek A., Scott J.L., Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R., Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D., Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Science 273:1058-1073(1996) [PubMed: 8688087] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.

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Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

L77117 Genomic DNA. Translation: AAB98611.1.

PIR

H64376.

RefSeq

NP_247600.1. NC_000909.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2YWX	X-ray	2.31	A	1-157	[»]

ProteinModelPortal

Q58033.

SMR

Q58033. Positions 1-157.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

1451482.

GenomeReviews

Gene locus MJ0616 in contig L77117_GR.

KEGG

mja:MJ_0616.

NMPDR

fig|243232.1.peg.637.

TIGR

MJ0616.

Phylogenomic databases

HOGENOM

HBG301965.

OMA

LIMGSDS.

ProtClustDB

CLSK876265.

Enzyme and pathway databases

BioCyc

MJAN243232:MJ_0616-MONOMER.

Family and domain databases

InterPro

IPR024694. N5-CAIR_mutase_PurE.
IPR000031. N5-CAIR_Mutase_PurE_dom.
[Graphical view]

Gene3D

G3DSA:3.40.50.7700. AIR_carboxyl. 1 hit.

KO

K01588.

Pfam

PF00731. AIRC. 1 hit.
[Graphical view]

PIRSF

PIRSF001338. AIR_carboxylase. 1 hit.

SMART

SM01001. AIRC. 1 hit.
[Graphical view]

SUPFAM

SSF52255. AIR_carboxyl. 1 hit.

TIGRFAMs

TIGR01162. PurE. 1 hit.

ProtoNet

Search...

Entry information

Entry name

PURE_METJA

Accession

Primary (citable) accession number: Q58033

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	January 25, 2012
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program