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Q58030

- END3_METJA

UniProt

Q58030 - END3_METJA

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Protein

Endonuclease III

Gene

nth

Organism
Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

DNA repair enzyme that has both DNA N-glycosylase activity and AP-lyase activity. The DNA N-glycosylase activity releases various damaged pyrimidines from DNA by cleaving the N-glycosidic bond, leaving an AP (apurinic/apyrimidinic) site. The AP-lyase activity cleaves the phosphodiester bond 3' to the AP site by a beta-elimination, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.UniRule annotation

Catalytic activityi

The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi184 – 1841Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi190 – 1901Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi193 – 1931Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi201 – 2011Iron-sulfur (4Fe-4S)UniRule annotation

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. DNA-(apurinic or apyrimidinic site) lyase activity Source: UniProtKB-HAMAP
  3. DNA binding Source: UniProtKB-HAMAP
  4. DNA N-glycosylase activity Source: UniProtKB-HAMAP
  5. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. base-excision repair Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase, Lyase

Keywords - Biological processi

DNA damage, DNA repair

Keywords - Ligandi

4Fe-4S, DNA-binding, Iron, Iron-sulfur, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Endonuclease IIIUniRule annotation (EC:4.2.99.18UniRule annotation)
Alternative name(s):
DNA-(apurinic or apyrimidinic site) lyaseUniRule annotation
Gene namesi
Name:nthUniRule annotation
Ordered Locus Names:MJ0613
OrganismiMethanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Taxonomic identifieri243232 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanococciMethanococcalesMethanocaldococcaceaeMethanocaldococcus
ProteomesiUP000000805: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 344344Endonuclease IIIPRO_0000102243Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi243232.MJ0613.

Structurei

3D structure databases

ProteinModelPortaliQ58030.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini105 – 12420HhHUniRule annotationAdd
BLAST
Domaini229 – 328100GIY-YIGAdd
BLAST

Sequence similaritiesi

Belongs to the Nth/MutY family.UniRule annotation
Contains 1 GIY-YIG domain.Curated
Contains 1 HhH domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG1833.
InParanoidiQ58030.
KOiK10773.
OMAiKLPKKYW.
PhylomeDBiQ58030.

Family and domain databases

Gene3Di1.10.1670.10. 1 hit.
1.10.340.30. 1 hit.
HAMAPiMF_00942. Nth.
InterProiIPR011257. DNA_glycosylase.
IPR002837. DUF123.
IPR004036. Endonuclease-III-like_CS2.
IPR003651. Endouclease3_FeS-loop_motif.
IPR000305. GIY-YIG_SF.
IPR003265. HhH-GPD_domain.
IPR000445. HhH_motif.
IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif.
IPR023170. HTH_base_excis_C.
IPR005759. Nth.
[Graphical view]
PfamiPF01986. DUF123. 1 hit.
PF00633. HHH. 1 hit.
PF00730. HhH-GPD. 1 hit.
[Graphical view]
SMARTiSM00478. ENDO3c. 1 hit.
SM00525. FES. 1 hit.
SM00465. GIYc. 1 hit.
SM00278. HhH1. 1 hit.
[Graphical view]
SUPFAMiSSF48150. SSF48150. 1 hit.
PROSITEiPS01155. ENDONUCLEASE_III_2. 1 hit.
PS50164. GIY_YIG. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q58030-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MELIEILLKK LNKNAVVTEI AKDKDPFKVL ISTIISARTK DEVTEEVSKK
60 70 80 90 100
LFKEIKDVDD LLNIDEEKLA DLIYPAGFYK NKAKNLKKLA KILKENYNGK
110 120 130 140 150
VPDSLEELLK LPGVGRKTAN LVITLAFNKD GICVDTHVHR ICNRWEIVDT
160 170 180 190 200
ETPEETEFEL RKKLPKKYWK VINNLLVVFG REICSSKSKC DKCFKEIKEK
210 220 230 240 250
CPYYEKIKHF ENILKKFNFR KVSKNKIPNE KGTYILKIRL KEGKKIKFGK
260 270 280 290 300
TERFFKKGYY FYIGSAFGNS MNLKNRIERH LKDDKKMHWH IDYLLKYGKI
310 320 330 340
EEIYITNERV ECEVANEFIK KFDFVENFGC SDCKCKSHLF YLKP
Length:344
Mass (Da):40,568
Last modified:December 15, 1998 - v2
Checksum:iBC3BEFE1DE778B85
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L77117 Genomic DNA. Translation: AAB98606.1.
RefSeqiNP_247597.1. NC_000909.1.
WP_010870118.1. NC_000909.1.

Genome annotation databases

EnsemblBacteriaiAAB98606; AAB98606; MJ_0613.
GeneIDi1451479.
KEGGimja:MJ_0613.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L77117 Genomic DNA. Translation: AAB98606.1 .
RefSeqi NP_247597.1. NC_000909.1.
WP_010870118.1. NC_000909.1.

3D structure databases

ProteinModelPortali Q58030.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 243232.MJ0613.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAB98606 ; AAB98606 ; MJ_0613 .
GeneIDi 1451479.
KEGGi mja:MJ_0613.

Phylogenomic databases

eggNOGi COG1833.
InParanoidi Q58030.
KOi K10773.
OMAi KLPKKYW.
PhylomeDBi Q58030.

Family and domain databases

Gene3Di 1.10.1670.10. 1 hit.
1.10.340.30. 1 hit.
HAMAPi MF_00942. Nth.
InterProi IPR011257. DNA_glycosylase.
IPR002837. DUF123.
IPR004036. Endonuclease-III-like_CS2.
IPR003651. Endouclease3_FeS-loop_motif.
IPR000305. GIY-YIG_SF.
IPR003265. HhH-GPD_domain.
IPR000445. HhH_motif.
IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif.
IPR023170. HTH_base_excis_C.
IPR005759. Nth.
[Graphical view ]
Pfami PF01986. DUF123. 1 hit.
PF00633. HHH. 1 hit.
PF00730. HhH-GPD. 1 hit.
[Graphical view ]
SMARTi SM00478. ENDO3c. 1 hit.
SM00525. FES. 1 hit.
SM00465. GIYc. 1 hit.
SM00278. HhH1. 1 hit.
[Graphical view ]
SUPFAMi SSF48150. SSF48150. 1 hit.
PROSITEi PS01155. ENDONUCLEASE_III_2. 1 hit.
PS50164. GIY_YIG. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.

Entry informationi

Entry nameiEND3_METJA
AccessioniPrimary (citable) accession number: Q58030
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: December 15, 1998
Last modified: November 26, 2014
This is version 91 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Methanococcus jannaschii
    Methanococcus jannaschii: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3