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Q58030 (END3_METJA) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 80. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Endonuclease III
EC=4.2.99.18
Alternative name(s):
DNA-(apurinic or apyrimidinic site) lyase
Gene names
Name:nth
Ordered Locus Names:MJ0613
OrganismMethanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii) [Reference proteome] [HAMAP]
Taxonomic identifier243232 [NCBI]
Taxonomic lineageArchaea › Euryarchaeota › Methanococci › Methanococcales › Methanocaldococcaceae › Methanocaldococcus ›

Protein attributes

Sequence length344 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

DNA repair enzyme that has both DNA N-glycosylase activity and AP-lyase activity. The DNA N-glycosylase activity releases various damaged pyrimidines from DNA by cleaving the N-glycosidic bond, leaving an AP (apurinic/apyrimidinic) site. The AP-lyase activity cleaves the phosphodiester bond 3' to the AP site by a beta-elimination, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate By similarity. HAMAP-Rule MF_00942

Catalytic activity

The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate. HAMAP-Rule MF_00942

Cofactor

Binds 1 4Fe-4S cluster By similarity.

Sequence similarities

Belongs to the Nth/MutY family.

Contains 1 GIY-YIG domain.

Contains 1 HhH domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 344344Endonuclease III HAMAP-Rule MF_00942
PRO_0000102243

Regions

Domain105 – 12420HhH
Domain229 – 328100GIY-YIG

Sites

Metal binding1841Iron-sulfur (4Fe-4S) By similarity
Metal binding1901Iron-sulfur (4Fe-4S) By similarity
Metal binding1931Iron-sulfur (4Fe-4S) By similarity
Metal binding2011Iron-sulfur (4Fe-4S) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q58030 [UniParc].

Last modified December 15, 1998. Version 2.
Checksum: BC3BEFE1DE778B85

FASTA34440,568
        10         20         30         40         50         60 
MELIEILLKK LNKNAVVTEI AKDKDPFKVL ISTIISARTK DEVTEEVSKK LFKEIKDVDD 

        70         80         90        100        110        120 
LLNIDEEKLA DLIYPAGFYK NKAKNLKKLA KILKENYNGK VPDSLEELLK LPGVGRKTAN 

       130        140        150        160        170        180 
LVITLAFNKD GICVDTHVHR ICNRWEIVDT ETPEETEFEL RKKLPKKYWK VINNLLVVFG 

       190        200        210        220        230        240 
REICSSKSKC DKCFKEIKEK CPYYEKIKHF ENILKKFNFR KVSKNKIPNE KGTYILKIRL 

       250        260        270        280        290        300 
KEGKKIKFGK TERFFKKGYY FYIGSAFGNS MNLKNRIERH LKDDKKMHWH IDYLLKYGKI 

       310        320        330        340 
EEIYITNERV ECEVANEFIK KFDFVENFGC SDCKCKSHLF YLKP

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		L77117 Genomic DNA. Translation: AAB98606.1.
RefSeqNP_247597.1. NC_000909.1.

3D structure databases

ProteinModelPortalQ58030.
ModBaseSearch...

Protein-protein interaction databases

STRING243232.MJ0613.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAB98606; AAB98606; MJ_0613.
GeneID1451479.
KEGGmja:MJ_0613.

Phylogenomic databases

eggNOGCOG1833.
KOK10773.
OMAKLPKKYW.
ProtClustDBCLSK876263.

Family and domain databases

Gene3D1.10.1670.10. 1 hit.
1.10.340.30. 1 hit.
HAMAPMF_00942. Nth.
InterProIPR011257. DNA_glycosylase.
IPR002837. DUF123.
IPR004036. Endonuclease-III_CS2.
IPR003651. Endouclease3_FeS-loop_motif.
IPR027299. GIY-YIG_dom.
IPR000305. GIY-YIG_SF.
IPR003265. HhH-GPD_domain.
IPR000445. HhH_motif.
IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif.
IPR023170. HTH_base_excis_C.
[Graphical view]
PfamPF01986. DUF123. 1 hit.
PF00633. HHH. 1 hit.
PF00730. HhH-GPD. 1 hit.
[Graphical view]
SMARTSM00478. ENDO3c. 1 hit.
SM00525. FES. 1 hit.
SM00465. GIYc. 1 hit.
SM00278. HhH1. 1 hit.
[Graphical view]
SUPFAMSSF48150. DNA_glycsylse. 1 hit.
PROSITEPS00764. ENDONUCLEASE_III_1. False negative.
PS01155. ENDONUCLEASE_III_2. 1 hit.
PS50164. GIY_YIG. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameEND3_METJA
AccessionPrimary (citable) accession number: Q58030
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: December 15, 1998
Last modified: May 1, 2013
This is version 80 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Methanococcus jannaschii

Methanococcus jannaschii: entries and gene names

SIMILARITY comments

Index of protein domains and families

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