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Q58030

- END3_METJA

UniProt

Q58030 - END3_METJA

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Protein

Endonuclease III

Gene
nth, MJ0613
Organism
Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

DNA repair enzyme that has both DNA N-glycosylase activity and AP-lyase activity. The DNA N-glycosylase activity releases various damaged pyrimidines from DNA by cleaving the N-glycosidic bond, leaving an AP (apurinic/apyrimidinic) site. The AP-lyase activity cleaves the phosphodiester bond 3' to the AP site by a beta-elimination, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate By similarity.UniRule annotation

Catalytic activityi

The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi184 – 1841Iron-sulfur (4Fe-4S) By similarity
Metal bindingi190 – 1901Iron-sulfur (4Fe-4S) By similarity
Metal bindingi193 – 1931Iron-sulfur (4Fe-4S) By similarity
Metal bindingi201 – 2011Iron-sulfur (4Fe-4S) By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. DNA-(apurinic or apyrimidinic site) lyase activity Source: UniProtKB-HAMAP
  3. DNA binding Source: UniProtKB-HAMAP
  4. DNA N-glycosylase activity Source: UniProtKB-HAMAP
  5. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. base-excision repair Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase, Lyase

Keywords - Biological processi

DNA damage, DNA repair

Keywords - Ligandi

4Fe-4S, DNA-binding, Iron, Iron-sulfur, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Endonuclease III (EC:4.2.99.18)
Alternative name(s):
DNA-(apurinic or apyrimidinic site) lyase
Gene namesi
Name:nth
Ordered Locus Names:MJ0613
OrganismiMethanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Taxonomic identifieri243232 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanococciMethanococcalesMethanocaldococcaceaeMethanocaldococcus
ProteomesiUP000000805: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 344344Endonuclease IIIUniRule annotationPRO_0000102243Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi243232.MJ0613.

Structurei

3D structure databases

ProteinModelPortaliQ58030.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini105 – 12420HhHAdd
BLAST
Domaini229 – 328100GIY-YIGAdd
BLAST

Sequence similaritiesi

Belongs to the Nth/MutY family.
Contains 1 GIY-YIG domain.
Contains 1 HhH domain.

Phylogenomic databases

eggNOGiCOG1833.
KOiK10773.
OMAiKLPKKYW.
PhylomeDBiQ58030.

Family and domain databases

Gene3Di1.10.1670.10. 1 hit.
1.10.340.30. 1 hit.
HAMAPiMF_00942. Nth.
InterProiIPR011257. DNA_glycosylase.
IPR002837. DUF123.
IPR004036. Endonuclease-III-like_CS2.
IPR003651. Endouclease3_FeS-loop_motif.
IPR000305. GIY-YIG_SF.
IPR003265. HhH-GPD_domain.
IPR000445. HhH_motif.
IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif.
IPR023170. HTH_base_excis_C.
IPR005759. Nth.
[Graphical view]
PfamiPF01986. DUF123. 1 hit.
PF00633. HHH. 1 hit.
PF00730. HhH-GPD. 1 hit.
[Graphical view]
SMARTiSM00478. ENDO3c. 1 hit.
SM00525. FES. 1 hit.
SM00465. GIYc. 1 hit.
SM00278. HhH1. 1 hit.
[Graphical view]
SUPFAMiSSF48150. SSF48150. 1 hit.
PROSITEiPS01155. ENDONUCLEASE_III_2. 1 hit.
PS50164. GIY_YIG. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q58030-1 [UniParc]FASTAAdd to Basket

« Hide

MELIEILLKK LNKNAVVTEI AKDKDPFKVL ISTIISARTK DEVTEEVSKK    50
LFKEIKDVDD LLNIDEEKLA DLIYPAGFYK NKAKNLKKLA KILKENYNGK 100
VPDSLEELLK LPGVGRKTAN LVITLAFNKD GICVDTHVHR ICNRWEIVDT 150
ETPEETEFEL RKKLPKKYWK VINNLLVVFG REICSSKSKC DKCFKEIKEK 200
CPYYEKIKHF ENILKKFNFR KVSKNKIPNE KGTYILKIRL KEGKKIKFGK 250
TERFFKKGYY FYIGSAFGNS MNLKNRIERH LKDDKKMHWH IDYLLKYGKI 300
EEIYITNERV ECEVANEFIK KFDFVENFGC SDCKCKSHLF YLKP 344
Length:344
Mass (Da):40,568
Last modified:December 15, 1998 - v2
Checksum:iBC3BEFE1DE778B85
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L77117 Genomic DNA. Translation: AAB98606.1.
RefSeqiNP_247597.1. NC_000909.1.
WP_010870118.1. NC_000909.1.

Genome annotation databases

EnsemblBacteriaiAAB98606; AAB98606; MJ_0613.
GeneIDi1451479.
KEGGimja:MJ_0613.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L77117 Genomic DNA. Translation: AAB98606.1 .
RefSeqi NP_247597.1. NC_000909.1.
WP_010870118.1. NC_000909.1.

3D structure databases

ProteinModelPortali Q58030.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 243232.MJ0613.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAB98606 ; AAB98606 ; MJ_0613 .
GeneIDi 1451479.
KEGGi mja:MJ_0613.

Phylogenomic databases

eggNOGi COG1833.
KOi K10773.
OMAi KLPKKYW.
PhylomeDBi Q58030.

Family and domain databases

Gene3Di 1.10.1670.10. 1 hit.
1.10.340.30. 1 hit.
HAMAPi MF_00942. Nth.
InterProi IPR011257. DNA_glycosylase.
IPR002837. DUF123.
IPR004036. Endonuclease-III-like_CS2.
IPR003651. Endouclease3_FeS-loop_motif.
IPR000305. GIY-YIG_SF.
IPR003265. HhH-GPD_domain.
IPR000445. HhH_motif.
IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif.
IPR023170. HTH_base_excis_C.
IPR005759. Nth.
[Graphical view ]
Pfami PF01986. DUF123. 1 hit.
PF00633. HHH. 1 hit.
PF00730. HhH-GPD. 1 hit.
[Graphical view ]
SMARTi SM00478. ENDO3c. 1 hit.
SM00525. FES. 1 hit.
SM00465. GIYc. 1 hit.
SM00278. HhH1. 1 hit.
[Graphical view ]
SUPFAMi SSF48150. SSF48150. 1 hit.
PROSITEi PS01155. ENDONUCLEASE_III_2. 1 hit.
PS50164. GIY_YIG. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.

Entry informationi

Entry nameiEND3_METJA
AccessioniPrimary (citable) accession number: Q58030
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: December 15, 1998
Last modified: September 3, 2014
This is version 88 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Methanococcus jannaschii
    Methanococcus jannaschii: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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