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Protein

Type-2 restriction enzyme MjaIII

Gene

mjaIIIR

Organism
Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Recognizes the double-stranded unmethylated sequence GATC and cleaves before G-1.

Catalytic activityi

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionEndonuclease, Hydrolase, Nuclease
Biological processRestriction system

Protein family/group databases

REBASEi3891. MjaIII.

Names & Taxonomyi

Protein namesi
Recommended name:
Type-2 restriction enzyme MjaIII (EC:3.1.21.4)
Short name:
R.MjaIII
Alternative name(s):
Endonuclease MjaIII
Type II restriction enzyme MjaIII
Gene namesi
Name:mjaIIIR
Ordered Locus Names:MJ0600
OrganismiMethanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Taxonomic identifieri243232 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanococciMethanococcalesMethanocaldococcaceaeMethanocaldococcus
Proteomesi
  • UP000000805 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000773341 – 290Type-2 restriction enzyme MjaIIIAdd BLAST290

Proteomic databases

PRIDEiQ58017.

Interactioni

Protein-protein interaction databases

STRINGi243232.MJ_0600.

Structurei

3D structure databases

ProteinModelPortaliQ58017.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiarCOG06575. Archaea.
ENOG410YTH4. LUCA.
KOiK01155.
OMAiGLEQIFC.
OrthoDBiPOG093Z05EO.

Family and domain databases

InterProiView protein in InterPro
IPR021191. Restrct_endonuc_II_DpmII.
IPR007637. Restrct_endonuc_II_DpmII-like.
PfamiView protein in Pfam
PF04556. DpnII. 1 hit.
PIRSFiPIRSF016080. Restrict_endonuc_II_DpmII. 1 hit.

Sequencei

Sequence statusi: Complete.

Q58017-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNFEYIINSL LETIKTYNFF VDWEKIENNI KKIEKRLHIL NYLIGKENFK
60 70 80 90 100
EEFFELLKEY PEVITVFPIL IAVRDNKITI LNENMELETL EFKEKKYLTD
110 120 130 140 150
EEIERYYKFF KETGLEDLLK NRKIKNLVDY VFGVEVGMDT NARKNRIGDL
160 170 180 190 200
MENIVKKYIE NLCKQNKNLD YIFQATKDKI KQKWGINLTL DKTNRKFDFA
210 220 230 240 250
VFNKNTKKLY LIEVNFYSGG GSKLKATAGE YRSLNEFIKN NNNNVQFIWI
260 270 280 290
TDGKGWNTAK NPLKESFNSG VVILNLKMVK EGLLKEILTQ
Length:290
Mass (Da):34,392
Last modified:November 1, 1996 - v1
Checksum:iACEBAE31B029900D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L77117 Genomic DNA. Translation: AAB98591.1.
PIRiH64374.
RefSeqiWP_010870104.1. NC_000909.1.

Genome annotation databases

EnsemblBacteriaiAAB98591; AAB98591; MJ_0600.
GeneIDi1451465.
KEGGimja:MJ_0600.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiT2M3_METJA
AccessioniPrimary (citable) accession number: Q58017
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: June 7, 2017
This is version 93 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Methanococcus jannaschii
    Methanococcus jannaschii: entries and gene names
  2. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  3. SIMILARITY comments
    Index of protein domains and families