Reviewed, UniProtKB/Swiss-Prot Q58017 (T2M3_METJA)

Names and origin

Protein names

Recommended name:
    Type-2 restriction enzyme MjaIII
      Short name=R.MjaIII
    EC=3.1.21.4
Alternative name(s):
    Type II restriction enzyme MjaIII
    Endonuclease MjaIII

Gene names

Name:	mjaIIIR
Ordered Locus Names:	MJ0600

Organism

Methanocaldococcus jannaschii (Methanococcus jannaschii) [Complete proteome] [HAMAP]

Taxonomic identifier

2190 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanocaldococcaceae › Methanocaldococcus

Protein attributes

Sequence length	290 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Recognizes the double-stranded unmethylated sequence GATC and cleaves before G-1.
Catalytic activity	Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.
Sequence similarities	Belongs to the type II restriction enzyme DpnII family.

Ontologies

Keywords
Biological process	Restriction system
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro Type II site-specific deoxyribonuclease activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 290	290	Type-2 restriction enzyme MjaIII		PRO_0000077334

Sequences

	Sequence		Length	Mass (Da)
	Q58017-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: ACEBAE31B029900D	FASTA	290	34,392
	10 20 30 40 50 60 MNFEYIINSL LETIKTYNFF VDWEKIENNI KKIEKRLHIL NYLIGKENFK EEFFELLKEY 70 80 90 100 110 120 PEVITVFPIL IAVRDNKITI LNENMELETL EFKEKKYLTD EEIERYYKFF KETGLEDLLK 130 140 150 160 170 180 NRKIKNLVDY VFGVEVGMDT NARKNRIGDL MENIVKKYIE NLCKQNKNLD YIFQATKDKI 190 200 210 220 230 240 KQKWGINLTL DKTNRKFDFA VFNKNTKKLY LIEVNFYSGG GSKLKATAGE YRSLNEFIKN 250 260 270 280 290 NNNNVQFIWI TDGKGWNTAK NPLKESFNSG VVILNLKMVK EGLLKEILTQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii." Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G., Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R., Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R., Kirkness E.F., Weinstock K.G. , Merrick J.M., Glodek A., Scott J.L., Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R., Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D., Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Science 273:1058-1073(1996) [PubMed: 8688087] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.
[2]	"Method for screening restriction endonucleases." Noren C.J., Roberts R.J., Patti J., Byrd D.R., Morgan R.D. Patent number WO9911821, 11-MAR-1999 Cited for: CHARACTERIZATION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L77117 Genomic DNA. Translation: AAB98591.1.
PIR	H64374.
RefSeq	NP_247582.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1451465.
GenomeReviews	Gene locus MJ0600 in contig L77117_GR.
KEGG	mja:MJ0600.
NMPDR	fig|243232.1.peg.619.
TIGR	MJ0600.
Phylogenomic databases
HOGENOM	Q58017.
OMA	EVGLDTN.
Enzyme and pathway databases
BioCyc	MJAN243232:MJ_0600-MON.
BRENDA	3.1.21.4. 256362.
Family and domain databases
InterPro	IPR007637. Restrict_endonuc_II_DpmII. [Graphical view]
Pfam	PF04556. DpmII. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

T2M3_METJA

Accession

Primary (citable) accession number: Q58017

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	November 3, 2009
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)