Reviewed, UniProtKB/Swiss-Prot Q58015 (MTM3_METJA)

Names and origin

Protein names

Recommended name:
    Modification methylase MjaIII
      Short name=M.MjaIII
    EC=2.1.1.72
Alternative name(s):
    Adenine-specific methyltransferase MjaIII

Gene names

Name:	mjaIIIM
Ordered Locus Names:	MJ0598

Organism

Methanocaldococcus jannaschii (Methanococcus jannaschii) [Complete proteome] [HAMAP]

Taxonomic identifier

2190 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanocaldococcaceae › Methanocaldococcus

Protein attributes

Sequence length	289 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	This methylase recognizes the double-stranded sequence GATC, causes specific methylation on A-2 on both strands, and protects the DNA from cleavage by the MjaIII endonuclease Potential.
Catalytic activity	S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.
Sequence similarities	Belongs to the N(4)/N(6)-methyltransferase family.

Ontologies

Keywords
Biological process	Restriction system
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA methylation Inferred from electronic annotation. Source: InterPro DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro site-specific DNA-methyltransferase (adenine-specific) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 289	289	Modification methylase MjaIII		PRO_0000087976
Sites
	Binding site	9	1	S-adenosyl-L-methionine By similarity
	Binding site	13	1	S-adenosyl-L-methionine; via amide nitrogen By similarity
	Binding site	63	1	S-adenosyl-L-methionine By similarity
	Binding site	199	1	S-adenosyl-L-methionine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q58015-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: CA95493C5CED8E78	FASTA	289	34,235
	10 20 30 40 50 60 MEVKPFLKWA GGKTQILSQI EENLPKELKE GNIKKYIEPF VGGGAVLFYL LQKYEFKKVI 70 80 90 100 110 120 ISDINEDLML CYKVVKNDVD RLIEELSSLR DEFLSLDEEK RKEFYYKVRD DFNKNKNDCD 130 140 150 160 170 180 EVKRVAQFIF LNKTCYNGLY RVNKKGEFNV PYGRYKNPKI FDEQNLKNVS KLLKNVKILC 190 200 210 220 230 240 GDFEIVDEYV DAESFVYFDP PYKPLNKTSS FTSYTKYDFN DDDQIRLAKF YRKLDKRGAK 250 260 270 280 LMLSNSYNVD FFGKLYEGFN IKKVVAKRMI NCKGDKRKDG IYELLIMNY

References

[1]

"Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii." Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G., Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R., Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R., Kirkness E.F., Weinstock K.G. , Merrick J.M., Glodek A., Scott J.L., Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R., Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D., Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Science 273:1058-1073(1996) [PubMed: 8688087] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L77117 Genomic DNA. Translation: AAB98590.1.
PIR	F64374.
RefSeq	NP_247580.1.
3D structure databases
SMR	Q58015. Positions 3-289.
ModBase	Search...
Protein family/group databases
REBASE	3890. M.MjaIII.
Genome annotation databases
GeneID	1451463.
GenomeReviews	Gene locus MJ0598 in contig L77117_GR.
KEGG	mja:MJ0598.
NMPDR	fig|243232.1.peg.617.
TIGR	MJ0598.
Phylogenomic databases
HOGENOM	HBG586381.
OMA	WAGGKFR.
Enzyme and pathway databases
BRENDA	2.1.1.72. 256362.
Family and domain databases
InterPro	IPR002294. D12N6_MeTrfase. IPR012326. Dam. IPR002052. DNA_methylase_N6_adenine_CS. IPR012327. MeTrfase_D12. [Graphical view]
Pfam	PF02086. MethyltransfD12. 1 hit. [Graphical view]
PRINTS	PR00505. D12N6MTFRASE.
TIGRFAMs	TIGR00571. dam. 1 hit.
PROSITE	PS00092. N6_MTASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MTM3_METJA

Accession

Primary (citable) accession number: Q58015

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	February 9, 2010
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)