Q57RU3 (LIPA_SALCH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Lipoyl synthase
EC=2.8.1.8

Alternative name(s):
Lip-syn
Short name=LS
Lipoate synthase
Lipoic acid synthase
Sulfur insertion protein LipA

Gene names

Name:	lipA
Ordered Locus Names:	SCH_0662

Organism

Salmonella choleraesuis (strain SC-B67) [Complete proteome] [HAMAP]

Taxonomic identifier

321314 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella › Salmonella choleraesuis › Salmonella enterica I › Salmonella enterica subsp. enterica serovar Choleraesuis

Protein attributes

Sequence length	321 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives By similarity. HAMAP-Rule MF_00206
Catalytic activity	Protein N(6)-(octanoyl)lysine + 2 sulfur + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_00206
Cofactor	Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.
Pathway	Protein modification; protein lipoylation via endogenous pathway; protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein]: step 2/2. HAMAP-Rule MF_00206
Subcellular location	Cytoplasm Potential HAMAP-Rule MF_00206.
Sequence similarities	Belongs to the radical SAM superfamily. Lipoyl synthase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding S-adenosyl-L-methionine
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	protein lipoylation Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: HAMAP lipoate synthase activity Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 321	321	Lipoyl synthase HAMAP-Rule MF_00206		PRO_1000012269
Sites
	Metal binding	68	1	Iron-sulfur 1 (4Fe-4S) By similarity
	Metal binding	73	1	Iron-sulfur 1 (4Fe-4S) By similarity
	Metal binding	79	1	Iron-sulfur 1 (4Fe-4S) By similarity
	Metal binding	94	1	Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
	Metal binding	98	1	Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
	Metal binding	101	1	Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q57RU3 [UniParc]. Last modified May 10, 2005. Version 1. Checksum: F57DF09996E9309C	FASTA	321	36,042
	10 20 30 40 50 60 MSKPIVMERG VKYRDADKMA LIPVKNVVTE RDALLRKPEW MKIKLPADST RIQGIKAAMR 70 80 90 100 110 120 KNGLHSVCEE ASCPNLAECF NHGTATFMIL GAICTRRCPF CDVAHGRPVA PDAEEPQKLA 130 140 150 160 170 180 QTIADMALRY VVITSVDRDD LRDGGAQHFA DCITAIRAKS PEIKIETLVP DFRGRMDRAL 190 200 210 220 230 240 DILNATPPDV FNHNLENVPR IYRQVRPGAD YNWSLKLLER FKEAHPEIPT KSGLMVGLGE 250 260 270 280 290 300 TNAEIIEVMR DLRRHGVTML TLGQYLQPSR HHLPVQRYVS PEEFDEMKAE ALAMGFTHAA 310 320 CGPFVRSSYH ADLQAKGMEV K

References

[1]

"The genome sequence of Salmonella enterica serovar Choleraesuis, a highly invasive and resistant zoonotic pathogen." Chiu C.-H., Tang P., Chu C., Hu S., Bao Q., Yu J., Chou Y.-Y., Wang H.-S., Lee Y.-S. Nucleic Acids Res. 33:1690-1698(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SC-B67.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017220 Genomic DNA. Translation: AAX64568.1.
RefSeq	YP_215649.1. NC_006905.1.
3D structure databases
ProteinModelPortal	Q57RU3.
ModBase	Search...
Protein-protein interaction databases
STRING	321314.SC0662.
Proteomic databases
PRIDE	Q57RU3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAX64568; AAX64568; SCH_0662.
GeneID	3333094.
KEGG	sec:SC0662.
PATRIC	32321911. VBISalEnt136302_0970.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0320.
KO	K03644.
OMA	EEYVTPE.
ProtClustDB	PRK05481.
Enzyme and pathway databases
BioCyc	SENT321314:GJCS-691-MONOMER.
UniPathway	UPA00538; UER00593.
Family and domain databases
Gene3D	3.20.20.70. 1 hit.
HAMAP	MF_00206. Lipoyl_synth.
InterPro	IPR013785. Aldolase_TIM. IPR006638. Elp3/MiaB/NifB. IPR003698. Lipoyl_synth. IPR007197. rSAM. [Graphical view]
PANTHER	PTHR10949. PTHR10949. 1 hit.
Pfam	PF04055. Radical_SAM. 1 hit. [Graphical view]
PIRSF	PIRSF005963. Lipoyl_synth. 1 hit.
SMART	SM00729. Elp3. 1 hit. [Graphical view]
TIGRFAMs	TIGR00510. lipA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

LIPA_SALCH

Accession

Primary (citable) accession number: Q57RU3

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	May 10, 2005
Last modified:	May 1, 2013
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program