uvrB

Functionⁱ

The UvrABC repair system catalyzes the recognition and processing of DNA lesions. A damage recognition complex composed of 2 UvrA and 2 UvrB subunits scans DNA for abnormalities. Upon binding of the UvrA₂B₂ complex to a putative damaged site, the DNA wraps around one UvrB monomer. DNA wrap is dependent on ATP binding by UvrB and probably causes local melting of the DNA helix, facilitating insertion of UvrB beta-hairpin between the DNA strands. Then UvrB probes one DNA strand for the presence of a lesion. If a lesion is found the UvrA subunits dissociate and the UvrB-DNA preincision complex is formed. This complex is subsequently bound by UvrC and the second UvrB is released. If no lesion is found, the DNA wraps around the other UvrB subunit that will check the other stand for damage.UniRule annotation

Regions

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Nucleotide bindingⁱ	39 – 46	ATPUniRule annotation		8

GO - Molecular functionⁱ

ATP binding Source: UniProtKB-KW
DNA binding Source: InterPro
excinuclease ABC activity Source: InterPro

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

nucleotide-excision repair Source: InterPro
SOS response Source: UniProtKB-KW

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Excision nuclease
Biological process	DNA damage, DNA excision, DNA repair, SOS response
Ligand	ATP-binding, Nucleotide-binding

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: UvrABC system protein BUniRule annotation Short name: Protein UvrBUniRule annotation Alternative name(s): Excinuclease ABC subunit BUniRule annotation
Gene namesⁱ	Name:uvrBUniRule annotation Ordered Locus Names:SCH_0796
Organismⁱ	Salmonella choleraesuis (strain SC-B67)
Taxonomic identifierⁱ	321314 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Salmonella › Salmonella choleraesuis › Salmonella enterica I ›
Proteomesⁱ	UP000000538 Componentⁱ: Chromosome

Subcellular locationⁱ

Cytoplasm
UniRule annotation
Manual assertion according to rulesⁱ
- HAMAP-Rule:MF_00204

GO - Cellular componentⁱ

cytoplasm Source: UniProtKB-SubCell

View the complete GO annotation on QuickGO ...

Keywords - Cellular componentⁱ

Cytoplasm

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000227357	1 – 673	UvrABC system protein BAdd BLAST		673

Interactionⁱ

Subunit structureⁱ

Forms a heterotetramer with UvrA during the search for lesions. Interacts with UvrC in an incision complex.UniRule annotation

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q57RF9.
SMRⁱ	Q57RF9.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Length
Domainⁱ	26 – 414	Helicase ATP-bindingUniRule annotationAdd BLAST	389
Domainⁱ	431 – 597	Helicase C-terminalUniRule annotationAdd BLAST	167
Domainⁱ	633 – 668	UVRUniRule annotationAdd BLAST	36

Motif

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Motifⁱ	92 – 115	Beta-hairpinAdd BLAST		24

Domainⁱ

The beta-hairpin motif is involved in DNA binding.UniRule annotation

Sequence similaritiesⁱ

Belongs to the UvrB family.UniRule annotation

Phylogenomic databases

KEGG Orthology (KO) More...KOⁱ	K03702.
OMAⁱ	RYMHSEI.

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd00079. HELICc. 1 hit.
HAMAPⁱ	MF_00204. UvrB. 1 hit.
InterProⁱ	View protein in InterPro IPR006935. Helicase/UvrB_N. IPR014001. Helicase_ATP-bd. IPR001650. Helicase_C. IPR027417. P-loop_NTPase. IPR001943. UVR_dom. IPR036876. UVR_dom_sf. IPR004807. UvrB. IPR024759. UvrB_YAD/RRR_dom.
PANTHERⁱ	PTHR24029. PTHR24029. 1 hit.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00271. Helicase_C. 1 hit. PF04851. ResIII. 1 hit. PF02151. UVR. 1 hit. PF12344. UvrB. 1 hit.
SMARTⁱ	View protein in SMART SM00487. DEXDc. 1 hit. SM00490. HELICc. 1 hit.
SUPFAMⁱ	SSF46600. SSF46600. 1 hit. SSF52540. SSF52540. 3 hits.
TIGRFAMsⁱ	TIGR00631. uvrb. 1 hit.
PROSITEⁱ	View protein in PROSITE PS51192. HELICASE_ATP_BIND_1. 1 hit. PS51194. HELICASE_CTER. 1 hit. PS50151. UVR. 1 hit.

Sequenceⁱ

Sequence statusⁱ: Complete.

Q57RF9-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MSKPFKLNSA FKPSGDQPDA IRRLEEGLED GLAHQTLLGV TGSGKTFTIA 
        60         70         80         90        100
NVIADLQRPT MVLAPNKTLA AQLYGEMKEF FPENAVEYFV SYYDYYQPEA 
       110        120        130        140        150
YVPSSDTFIE KDASVNEHIE QMRLSATKAL LERRDVVVVA SVSAIYGLGD 
       160        170        180        190        200
PDLYLKMMLH LTVGMLIDQR AILRRLAELQ YTRNDQAFQR GTFRVRGEVI 
       210        220        230        240        250
DIFPAESDDI ALRVELFDEE VERLSLFDPL TGQVESTVPR YTIYPKTHYV 
       260        270        280        290        300
TPRERILQAM EEIKDELADR RKVLLANNKL LEEQRLSQRT QFDLEMMNEL 
       310        320        330        340        350
GYCSGIENYS RFLSGRGPGE PPPTLFDYLP ADGLLVVDES HVTIPQIGGM 
       360        370        380        390        400
YRGDRARKET LVEYGFRLPS ALDNRPLKFE EFEALAPQTI YVSATPGNYE 
       410        420        430        440        450
LEKSGDEVVD QVVRPTGLLD PIIEVRPVAT QVDDLLSEIR QRAAINERVL 
       460        470        480        490        500
VTTLTKRMAE DLTEYLEEHG ERVRYLHSDI DTVERMEIIR DLRLGEFDVL 
       510        520        530        540        550
VGINLLREGL DMPEVSLVAI LDADKEGFLR SERSLIQTIG RAARNVNGKA 
       560        570        580        590        600
ILYGDKITPS MAKAIGETER RREKQQKYNE EHGITPQGLN KKVVDILALG 
       610        620        630        640        650
QNIAKTKAKG KGKGRSTAKA GIVELDMTPK ALQQKIHELE GQMMQHAQNL 
       660        670 
EFEEAAQIRD QLHQLRELFI AAS

Length:673

Mass (Da):76,135

Last modified:May 10, 2005 - v1

Checksum:ⁱ054E205FADA6C890

GO

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AE017220 Genomic DNA. Translation: AAX64702.1.
NCBI Reference Sequences More...RefSeqⁱ	WP_000042502.1. NC_006905.1.

Genome annotation databases

EnsemblBacteriaⁱ	AAX64702; AAX64702; SCH_0796.
KEGGⁱ	sec:SCH_0796.

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
Q57RF9	B5F076 B5FP65 B5QX69 B5R765 B4SZK1 A9MTI2 C0PWY7 B4TQU3 Q8ZQQ4 A0A2D3TXZ1 +70	Salmonella agona (strain SL483) Salmonella dublin (strain CT_02021853) Salmonella enteritidis PT4 (strain P125109) Salmonella gallinarum (strain 287/91 / NCTC 13346) Salmonella newport (strain SL254) Salmonella paratyphi B (strain ATCC BAA-1250 / SPB7) Salmonella paratyphi C (strain RKS4594) Salmonella schwarzengrund (strain CVM19633) Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) Salmonella enterica subsp. enterica serovar Hayindogo And more	673	UniRef100_B5F076	Cluster: UvrABC system protein B	81

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
Q57RF9	Q8FJP8 A7MIZ7 A0A0F6VWK9 V5U1X8 A0A0U4GIU8 K8CTM7 K8BXR6 UPI0006207C3F I2WBN7 UPI000840B988 +1233	Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC) Cronobacter sakazakii (strain ATCC BAA-894) (Enterobacter sakazakii) Cronobacter sakazakii (Enterobacter sakazakii) Cronobacter malonaticus Cronobacter malonaticus LMG 23826 Cronobacter sakazakii 701 Cronobacter malonaticus 507 Salmonella choleraesuis Escherichia coli 9.0111 Escherichia coli And more	673	UniRef90_Q8FJP8	Cluster: UvrABC system protein B	1,244

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
Q57RF9	Q8FJP8 A7MIZ7 A0A0F6VWK9 V5U1X8 A0A0U4GIU8 K8CTM7 K8BXR6 UPI0006207C3F I2WBN7 UPI000840B988 +5456	Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC) Cronobacter sakazakii (strain ATCC BAA-894) (Enterobacter sakazakii) Cronobacter sakazakii (Enterobacter sakazakii) Cronobacter malonaticus Cronobacter malonaticus LMG 23826 Cronobacter sakazakii 701 Cronobacter malonaticus 507 Salmonella choleraesuis Escherichia coli 9.0111 Escherichia coli And more	673	UniRef50_Q8FJP8	Cluster: UvrABC system protein B	5,467

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AE017220 Genomic DNA. Translation: AAX64702.1.
NCBI Reference Sequences More...RefSeqⁱ	WP_000042502.1. NC_006905.1.

3D structure databases

ProteinModelPortalⁱ	Q57RF9.
SMRⁱ	Q57RF9.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblBacteriaⁱ	AAX64702; AAX64702; SCH_0796.
KEGGⁱ	sec:SCH_0796.

Phylogenomic databases

KEGG Orthology (KO) More...KOⁱ	K03702.
OMAⁱ	RYMHSEI.

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd00079. HELICc. 1 hit.
HAMAPⁱ	MF_00204. UvrB. 1 hit.
InterProⁱ	View protein in InterPro IPR006935. Helicase/UvrB_N. IPR014001. Helicase_ATP-bd. IPR001650. Helicase_C. IPR027417. P-loop_NTPase. IPR001943. UVR_dom. IPR036876. UVR_dom_sf. IPR004807. UvrB. IPR024759. UvrB_YAD/RRR_dom.
PANTHERⁱ	PTHR24029. PTHR24029. 1 hit.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00271. Helicase_C. 1 hit. PF04851. ResIII. 1 hit. PF02151. UVR. 1 hit. PF12344. UvrB. 1 hit.
SMARTⁱ	View protein in SMART SM00487. DEXDc. 1 hit. SM00490. HELICc. 1 hit.
SUPFAMⁱ	SSF46600. SSF46600. 1 hit. SSF52540. SSF52540. 3 hits.
TIGRFAMsⁱ	TIGR00631. uvrb. 1 hit.
PROSITEⁱ	View protein in PROSITE PS51192. HELICASE_ATP_BIND_1. 1 hit. PS51194. HELICASE_CTER. 1 hit. PS50151. UVR. 1 hit.
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	UVRB_SALCH
Accessionⁱ	Q57RF9Primary (citable) accession number: Q57RF9
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
	Last sequence update:	May 10, 2005
	Last modified:	February 28, 2018
	This is version 83 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Prokaryotic Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - Q57RF9 (UVRB_SALCH)

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UvrABC system protein B

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<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Regions

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Motif

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protocols and materials databases

Genome annotation databases

Phylogenomic databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

PTM / Processingⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ