UvrABC system protein B - Salmonella choleraesuis (strain SC-B67)

Preferred order of sections

Names and origin

Protein names

Recommended name:
UvrABC system protein B
Short name=Protein UvrB

Alternative name(s):
Excinuclease ABC subunit B

Gene names

Name:	uvrB
Ordered Locus Names:	SCH_0796

Organism

Salmonella choleraesuis (strain SC-B67) [Complete proteome] [HAMAP]

Taxonomic identifier

321314 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella ›

Protein attributes

Sequence length	673 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	The UvrABC repair system catalyzes the recognition and processing of DNA lesions. A damage recognition complex composed of 2 UvrA and 2 UvrB subunits scans DNA for abnormalities. Upon binding of the UvrA₂B₂ complex to a putative damaged site, the DNA wraps around one UvrB monomer. DNA wrap is dependent on ATP binding by UvrB and probably causes local melting of the DNA helix, facilitating insertion of UvrB beta-hairpin between the DNA strands. Then UvrB probes one DNA strand for the presence of a lesion. If a lesion is found the UvrA subunits dissociate and the UvrB-DNA preincision complex is formed. This complex is subsequently bound by UvrC and the second UvrB is released. If no lesion is found, the DNA wraps around the other UvrB subunit that will check the other stand for damage By similarity. HAMAP-Rule MF_00204
Subunit structure	Forms a heterotetramer with UvrA during the search for lesions. Interacts with UvrC in an incision complex By similarity.
Subcellular location	Cytoplasm By similarity.
Domain	The beta-hairpin motif is involved in DNA binding By similarity. HAMAP-Rule MF_00204
Sequence similarities	Belongs to the UvrB family. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain. Contains 1 UVR domain.

Ontologies

Keywords
Biological process	DNA damage DNA excision DNA repair SOS response
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Excision nuclease
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	SOS response Inferred from electronic annotation. Source: HAMAP nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC nucleotide-excision repair Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP DNA binding Inferred from electronic annotation. Source: HAMAP excinuclease ABC activity Inferred from electronic annotation. Source: HAMAP helicase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 673

673

UvrABC system protein B HAMAP-Rule MF_00204

PRO_0000227357

Regions

Domain

26 – 414

389

Helicase ATP-binding

Domain

431 – 597

167

Helicase C-terminal

Domain

633 – 668

36

UVR

Nucleotide binding

39 – 46

8

ATP Potential

Motif

92 – 115

24

Beta-hairpin HAMAP-Rule MF_00204

Sequences

Sequence

Length

Mass (Da)

Tools

Q57RF9 [UniParc].

Last modified May 10, 2005. Version 1.
Checksum: 054E205FADA6C890
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FASTA

673

76,135

        10         20         30         40         50         60 
MSKPFKLNSA FKPSGDQPDA IRRLEEGLED GLAHQTLLGV TGSGKTFTIA NVIADLQRPT 

        70         80         90        100        110        120 
MVLAPNKTLA AQLYGEMKEF FPENAVEYFV SYYDYYQPEA YVPSSDTFIE KDASVNEHIE 

       130        140        150        160        170        180 
QMRLSATKAL LERRDVVVVA SVSAIYGLGD PDLYLKMMLH LTVGMLIDQR AILRRLAELQ 

       190        200        210        220        230        240 
YTRNDQAFQR GTFRVRGEVI DIFPAESDDI ALRVELFDEE VERLSLFDPL TGQVESTVPR 

       250        260        270        280        290        300 
YTIYPKTHYV TPRERILQAM EEIKDELADR RKVLLANNKL LEEQRLSQRT QFDLEMMNEL 

       310        320        330        340        350        360 
GYCSGIENYS RFLSGRGPGE PPPTLFDYLP ADGLLVVDES HVTIPQIGGM YRGDRARKET 

       370        380        390        400        410        420 
LVEYGFRLPS ALDNRPLKFE EFEALAPQTI YVSATPGNYE LEKSGDEVVD QVVRPTGLLD 

       430        440        450        460        470        480 
PIIEVRPVAT QVDDLLSEIR QRAAINERVL VTTLTKRMAE DLTEYLEEHG ERVRYLHSDI 

       490        500        510        520        530        540 
DTVERMEIIR DLRLGEFDVL VGINLLREGL DMPEVSLVAI LDADKEGFLR SERSLIQTIG 

       550        560        570        580        590        600 
RAARNVNGKA ILYGDKITPS MAKAIGETER RREKQQKYNE EHGITPQGLN KKVVDILALG 

       610        620        630        640        650        660 
QNIAKTKAKG KGKGRSTAKA GIVELDMTPK ALQQKIHELE GQMMQHAQNL EFEEAAQIRD 

       670 
QLHQLRELFI AAS

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References

[1]

"The genome sequence of Salmonella enterica serovar Choleraesuis, a highly invasive and resistant zoonotic pathogen."
Chiu C.-H., Tang P., Chu C., Hu S., Bao Q., Yu J., Chou Y.-Y., Wang H.-S., Lee Y.-S.
Nucleic Acids Res. 33:1690-1698(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: SC-B67.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017220 Genomic DNA. Translation: AAX64702.1.
RefSeq	YP_215783.1. NC_006905.1.
3D structure databases
ProteinModelPortal	Q57RF9.
SMR	Q57RF9. Positions 5-596, 623-673.
ModBase	Search...
Protein-protein interaction databases
STRING	321314.SC0796.
Proteomic databases
PRIDE	Q57RF9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAX64702; AAX64702; SCH_0796.
GeneID	3333234.
KEGG	sec:SC0796.
PATRIC	32322236. VBISalEnt136302_1121.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0556.
KO	K03702.
OMA	CIYGLGI.
ProtClustDB	PRK05298.
Enzyme and pathway databases
BioCyc	SENT321314:GJCS-842-MONOMER.
Family and domain databases
Gene3D	4.10.860.10. 1 hit.
HAMAP	MF_00204. UvrB.
InterPro	IPR006935. Helicase/UvrB_dom. IPR014001. Helicase_ATP-bd. IPR001650. Helicase_C. IPR001943. UVR_dom. IPR004807. UvrB. IPR024759. UvrB_YAD/RRR_dom. [Graphical view]
PANTHER	PTHR24029. PTHR24029. 1 hit.
Pfam	PF00271. Helicase_C. 1 hit. PF04851. ResIII. 1 hit. PF02151. UVR. 1 hit. PF12344. UvrB. 1 hit. [Graphical view]
SMART	SM00487. DEXDc. 1 hit. SM00490. HELICc. 1 hit. [Graphical view]
SUPFAM	SSF46600. UvrB_C. 1 hit.
TIGRFAMs	TIGR00631. uvrb. 1 hit.
PROSITE	PS51192. HELICASE_ATP_BIND_1. 1 hit. PS51194. HELICASE_CTER. 1 hit. PS50151. UVR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UVRB_SALCH

Accession

Primary (citable) accession number: Q57RF9

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	May 10, 2005
Last modified:	May 1, 2013
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Entry information

Relevant documents