Reviewed, UniProtKB/Swiss-Prot Q57KH9 (CYSH_SALCH)

Names and origin

Protein names

Recommended name:
    Phosphoadenosine phosphosulfate reductase
    EC=1.8.4.8
Alternative name(s):
    PAPS reductase, thioredoxin dependent
    PAdoPS reductase
    3'-phosphoadenylylsulfate reductase
    PAPS sulfotransferase

Gene names

Name:	cysH
Ordered Locus Names:	SCH_2877

Organism

Salmonella choleraesuis [Complete proteome] [HAMAP]

Taxonomic identifier

28901 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella

Protein attributes

Sequence length	244 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Reduction of activated sulfate into sulfite. HAMAP MF_00063
Catalytic activity	Adenosine 3',5'-bisphosphate + sulfite + thioredoxin disulfide = 3'-phosphoadenylyl sulfate + thioredoxin. HAMAP MF_00063
Pathway	Sulfur metabolism; hydrogen sulfide biosynthesis; sulfite from sulfate: step 3/3. HAMAP MF_00063
Subcellular location	Cytoplasm By similarity HAMAP MF_00063.
Sequence similarities	Belongs to the PAPS reductase family. CysH subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cysteine biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW sulfate assimilation, phosphoadenylyl sulfate reduction by phosphoadenylyl-sulfate reductase (thioredoxin) Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	phosphoadenylyl-sulfate reductase (thioredoxin) activity Inferred from electronic annotation. Source: HAMAP transferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 244	244	Phosphoadenosine phosphosulfate reductase HAMAP MF_00063		PRO_1000008934

Sequences

	Sequence		Length	Mass (Da)
	Q57KH9-1 [UniParc]. Last modified May 10, 2005. Version 1. Checksum: E70117016708C42E	FASTA	244	27,968
	10 20 30 40 50 60 MSQLDLNALN ELPKVDRVLA LAEINAQLET LSAEERVAWA LENLPGEYVL SSSFGIQAAV 70 80 90 100 110 120 SLHLVNQIRP DIPVILTDTG YLFPETYQFI DELTDKLKLN LKVYRAGESP AWQEARYGKL 130 140 150 160 170 180 WEQGVEGIEK YNDINKVEPM NRALKELKAQ TWFAGLRREQ SGSRAHLPVL ATQRGVFKVL 190 200 210 220 230 240 PIIDWDNRTV YQYLQKHGLK YHPLWDQGYL SVGDTHTTRK WEPGMAEEET RFFGLKRECG LHEG

References

[1]

"The genome sequence of Salmonella enterica serovar Choleraesuis, a highly invasive and resistant zoonotic pathogen." Chiu C.-H., Tang P., Chu C., Hu S., Bao Q., Yu J., Chou Y.-Y., Wang H.-S., Lee Y.-S. Nucleic Acids Res. 33:1690-1698(2005) [PubMed: 15781495] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SC-B67.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017220 Genomic DNA. Translation: AAX66783.1.
RefSeq	YP_217864.1.
3D structure databases
HSSP	HSSP built from PDB template 1SUR based on UniProtKB P17854.
SMR	Q57KH9. Positions 2-244.
ModBase	Search...
Genome annotation databases
GeneID	3335383.
GenomeReviews	Gene locus SCH_2877 in contig AE017220_GR.
KEGG	sec:SC2877.
NMPDR	fig|321314.4.peg.3033.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG758022.
OMA	HYYLEEH.
Enzyme and pathway databases
BioCyc	SENT321314:SCH_2877-MONOMER.
BRENDA	1.8.4.8. 276841.
Family and domain databases
HAMAP	MF_00063. CysH. [Tree]
InterPro	IPR004511. PAdo_PSO4_Rdtase_CysH. IPR002500. PAPS_reduct. IPR011800. PAPS_reductase. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
Pfam	PF01507. PAPS_reduct. 1 hit. [Graphical view]
TIGRFAMs	TIGR00434. cysH. 1 hit. TIGR02057. PAPS_reductase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

CYSH_SALCH

Accession

Primary (citable) accession number: Q57KH9

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	May 10, 2005
Last modified:	February 9, 2010
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)