Q57G10 (DNAA_BRUAB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chromosomal replication initiator protein DnaA

Gene names

Name:	dnaA
Ordered Locus Names:	BruAb1_0001

Organism

Brucella abortus biovar 1 (strain 9-941) [Complete proteome] [HAMAP]

Taxonomic identifier

262698 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	496 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Plays an important role in the initiation and regulation of chromosomal replication. Binds to the origin of replication; it binds specifically double-stranded DNA at a 9 bp consensus (dnaA box): 5'-TTATC[CA]A[CA]A-3'. DnaA binds to ATP and to acidic phospholipids By similarity. HAMAP MF_00377
Subcellular location	Cytoplasm By similarity HAMAP MF_00377.
Sequence similarities	Belongs to the DnaA family.

Ontologies

Keywords
Biological process	DNA replication
Cellular component	Cytoplasm
Ligand	ATP-binding DNA-binding Nucleotide-binding
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA-dependent DNA replication initiation Inferred from electronic annotation. Source: InterPro regulation of DNA replication Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA replication origin binding Inferred from electronic annotation. Source: InterPro nucleoside-triphosphatase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 496	496	Chromosomal replication initiator protein DnaA HAMAP MF_00377		PRO_0000114145
Regions
	Nucleotide binding	194 – 201	8	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q57G10 [UniParc]. Last modified May 10, 2005. Version 1. Checksum: 4476E21259505ADA	FASTA	496	55,121
	10 20 30 40 50 60 MKMDSAVSEE AFERLTAKLK ARVGGEIYSS WFGRLKLDDI SKSIVRLSVP TAFLRSWINN 70 80 90 100 110 120 HYSELLTELW QEENPQILKV EVVVRGVSRV VRSAAPAETC DNAEAKPAVT PREKMVFPVG 130 140 150 160 170 180 QSFGGQSLGE KRGSAVVAES AAATGAVLGS PLDPRYTFDT FVDGASNRVA LAAARTIAEA 190 200 210 220 230 240 GSSAVRFNPL FIHASVGLGK THLLQAIAAA ALQRQEKARV VYLTAEYFMW RFATAIRDNN 250 260 270 280 290 300 ALSFKEQLRD IDLLVIDDMQ FLQGKSIQHE FCHLLNTLLD SAKQVVVAAD RAPSELESLD 310 320 330 340 350 360 VRVRSRLQGG VALEVAAPDY EMRLEMLRRR LASAQCEDAS LDIGEEILAH VARTVTGSGR 370 380 390 400 410 420 ELEGAFNQLL FRQSFEPNIS IDRVDELLGH LTRAGEPKRI RIEEIQRIVA RHYNVSKQDL 430 440 450 460 470 480 LSNRRTRTIV KPRQVAMYLA KMMTPRSLPE IGRRFGGRDH TTVLHAVRKI EDLVGADTKL 490 AQELELLKRL INDQAA

References

[1]

"Completion of the genome sequence of Brucella abortus and comparison to the highly similar genomes of Brucella melitensis and Brucella suis." Halling S.M., Peterson-Burch B.D., Bricker B.J., Zuerner R.L., Qing Z., Li L.-L., Kapur V., Alt D.P., Olsen S.C. J. Bacteriol. 187:2715-2726(2005) [PubMed: 15805518] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 9-941.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017223 Genomic DNA. Translation: AAX73424.1.
RefSeq	YP_220785.1. NC_006932.1.
3D structure databases
ProteinModelPortal	Q57G10.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3339217.
GenomeReviews	Gene locus BruAb1_0001 in contig AE017223_GR.
KEGG	bmb:BruAb1_0001.
PATRIC	17821513. VBIBruAbo15061_0002.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG695666.
OMA	CHTLNAL.
PhylomeDB	Q57G10.
ProtClustDB	PRK00149.
Enzyme and pathway databases
BioCyc	BABO262698:BRUAB1_0001-MONOMER.
Family and domain databases
HAMAP	MF_00377. DnaA_bact. [Tree]
InterPro	IPR003593. ATPase_AAA+_core. IPR001957. Chromosome_initiator_DnaA. IPR020591. Chromosome_initiator_DnaA-like. IPR018312. Chromosome_initiator_DnaA_CS. IPR013159. DnaA_C. IPR013317. DnaA_Hda. IPR010921. Trp_repressor/repl_initiator. [Graphical view]
Gene3D	G3DSA:1.10.1750.10. DnaA_C. 1 hit.
KO	K02313.
Pfam	PF00308. Bac_DnaA. 1 hit. PF08299. Bac_DnaA_C. 1 hit. [Graphical view]
PRINTS	PR00051. DNAA.
SMART	SM00382. AAA. 1 hit. SM00760. Bac_DnaA_C. 1 hit. [Graphical view]
SUPFAM	SSF48295. Trp_repress_rep. 1 hit.
TIGRFAMs	TIGR00362. DnaA. 1 hit.
PROSITE	PS01008. DNAA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DNAA_BRUAB

Accession

Primary (citable) accession number: Q57G10

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2005
Last sequence update:	May 10, 2005
Last modified:	January 25, 2012
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program