Reviewed, UniProtKB/Swiss-Prot Q57FX6 (KDSB_BRUAB)

Names and origin

Protein names

Recommended name:
    3-deoxy-manno-octulosonate cytidylyltransferase
    EC=2.7.7.38
Alternative name(s):
    CMP-KDO synthetase
    CMP-2-keto-3-deoxyoctulosonic acid synthetase
      Short name=CKS

Gene names

Name:	kdsB
Ordered Locus Names:	BruAb1_0038

Organism

Brucella abortus [Complete proteome] [HAMAP]

Taxonomic identifier

235 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	251 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Activates KDO (a required 8-carbon sugar) for incorporation into bacterial lipopolysaccharide in Gram-negative bacteria By similarity.
Catalytic activity	CTP + 3-deoxy-D-manno-octulosonate = diphosphate + CMP-3-deoxy-D-manno-octulosonate. HAMAP MF_00057
Pathway	Nucleotide-sugar biosynthesis; CMP-3-deoxy-D-manno-octulosonate biosynthesis; CMP-3-deoxy-D-manno-octulosonate from 3-deoxy-D-manno-octulosonate and CTP: step 1/1. HAMAP MF_00057 Bacterial outer membrane biogenesis; lipopolysaccharide biosynthesis. HAMAP MF_00057
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the kdsB family.

Ontologies

Keywords
Biological process	Lipopolysaccharide biosynthesis
Cellular component	Cytoplasm
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	keto-3-deoxy-D-manno-octulosonic acid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-deoxy-manno-octulosonate cytidylyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 251	251	3-deoxy-manno-octulosonate cytidylyltransferase HAMAP MF_00057		PRO_0000370011

Sequences

	Sequence		Length	Mass (Da)
	Q57FX6-1 [UniParc]. Last modified May 10, 2005. Version 1. Checksum: 945714A32037C6A7	FASTA	251	27,304
	10 20 30 40 50 60 MLQTMKTLTL IPARLGSTRL PNKPLADICG KPMIVHVADR AAAAKLGRTV IATDSEEIFK 70 80 90 100 110 120 VVAAHGHEAI MTRGDHESGS DRIYEALAKL DPSGEIDAVV NVQGDLPTID PDTIRRALLP 130 140 150 160 170 180 LEDGPADIAT LGVEITVEEE KTNPNVVKIV GSPLAGNRRL RALYFTRATA PYGEGPLYHH 190 200 210 220 230 240 IGLYAYRRSA LERFVKLGPS PLEKREKLEQ LRALEAGMRI DVEIVKTVPL GVDTQADLDR 250 ARTFCSQAGT I

References

[1]

"Completion of the genome sequence of Brucella abortus and comparison to the highly similar genomes of Brucella melitensis and Brucella suis." Halling S.M., Peterson-Burch B.D., Bricker B.J., Zuerner R.L., Qing Z., Li L.-L., Kapur V., Alt D.P., Olsen S.C. J. Bacteriol. 187:2715-2726(2005) [PubMed: 15805518] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 9-941 / Biovar 1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE017223 Genomic DNA. Translation: AAX73458.1.
RefSeq	YP_220819.1.
3D structure databases
HSSP	HSSP built from PDB template 1VH1 based on UniProtKB P04951.
ModBase	Search...
Genome annotation databases
GeneID	3339238.
GenomeReviews	Gene locus BruAb1_0038 in contig AE017223_GR.
KEGG	bmb:BruAb1_0038.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q57FX6.
OMA	TDRINEA
Enzyme and pathway databases
BioCyc	BABO262698:BRUAB1_0038-MON.
Family and domain databases
HAMAP	MF_00057. [Tree]
InterPro	IPR003329. Cytidylyl_trans. IPR004528. KdsB. [Graphical view]
Pfam	PF02348. CTP_transf_3. 1 hit. [Graphical view]
TIGRFAMs	TIGR00466. kdsB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

KDSB_BRUAB

Accession

Primary (citable) accession number: Q57FX6

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	May 10, 2005
Last modified:	November 24, 2009
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)