Reviewed, UniProtKB/Swiss-Prot Q57FU7 (DNAE2_BRUAB)

Names and origin

Protein names

Recommended name:
    Error-prone DNA polymerase
    EC=2.7.7.7

Gene names

Name:	dnaE2
Ordered Locus Names:	BruAb1_0069

Organism

Brucella abortus [Complete proteome] [HAMAP]

Taxonomic identifier

235 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	1077 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	DNA polymerase involved in damage-induced mutagenesis and translesion synthesis (TLS). It is not the major replicative DNA polymerase By similarity. HAMAP MF_01902
Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). HAMAP MF_01902
Subcellular location	Cytoplasm Probable HAMAP MF_01902.
Sequence similarities	Belongs to the DNA polymerase type-C family. DnaE2 subfamily.

Ontologies

Keywords
Biological process	DNA damage DNA repair DNA replication
Cellular component	Cytoplasm
Molecular function	DNA-directed DNA polymerase Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA repair Inferred from electronic annotation. Source: UniProtKB-KW DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3'-5' exonuclease activity Inferred from electronic annotation. Source: InterPro DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed DNA polymerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1077	1077	Error-prone DNA polymerase HAMAP MF_01902		PRO_0000103370

Sequences

	Sequence		Length	Mass (Da)
	Q57FU7-1 [UniParc]. Last modified May 10, 2005. Version 1. Checksum: 174FFECD599A8B3A	FASTA	1,077	120,966
	10 20 30 40 50 60 MVPYFEMAAA SNFSFLCGAS HPQELVERAH ALDLSGIGIA DRNTLAGVVR AHAQWKDIRK 70 80 90 100 110 120 ESGFRLFIGC RLSFIDGTPD MVVYPRDRAA YGQLCRLLTE GKHRAAIKGE CHLEWADLLF 130 140 150 160 170 180 RARQFQIAVF PPDEDEPDFA ARLTEIAQAA PGSVWLALTM PHQGQDGRRA ERIARFAAQA 190 200 210 220 230 240 GVPLIATNDV LYHHPDRRPL QDVLTATRHH TTVFAAGRLL EKNAERHLKP PHEMVRLFRD 250 260 270 280 290 300 YPEAIAATAD FVAPITFQLD ELKYAYPDEP IPPGKTAQQH LYDLVWEGAA RHYGADMIPP 310 320 330 340 350 360 KVQGLINKEL ALIARLEYEP YFLTVYDIVT HAREKGILCQ GRGSAANSVV CFCLGITGVN 370 380 390 400 410 420 PTQVDLLFER FISAERKEPP DIDVDFEHER REEVMQYVYD RYSRDRAAIV ATVISYRSRS 430 440 450 460 470 480 AIRDVGKALG LSEDVTAALA NTVWGLSGGG IDRQHIRQAG LDPDNPIIQR AVELAITLIG 490 500 510 520 530 540 FPRHLSQHVG GFVLTRDRLD ETVPIGPAAM DKRSFIEWDK DDIDEVGLMK VDVLSLGMLT 550 560 570 580 590 600 CIRKAFDLIH QHKPQLYGGE KLTLASLPRK DKAVYDMLCK GDSLGVFQVE SRAQMNMLPR 610 620 630 640 650 660 LRPQEFYDLV IEVAIVRPGP IQGDMVHPYL RRRSGQEPCT LPSPSPQHGP ANELQQILGK 670 680 690 700 710 720 TKGVPLFQEQ AMRIAMEAAK FTPEEANQLR RAMATFRKMG TIHTMEKKMI DGMVNRGYDR 730 740 750 760 770 780 TFAENCFNQI KGFGEYGFPE SHAASFAHLV YISAWLKCHH PEVFAAALLN SQPMGFYAPA 790 800 810 820 830 840 QIVRDAREHG VTVLPVDVNF SQWDNILEET PDVHLALRLG FRQIDGFSKR DTELLIADRQ 850 860 870 880 890 900 EPYRTIEDMH RRLRLDRRAF TLLADADAFG SLDIDRRAAL WAVRRLPNDE TLPLFRAAAA 910 920 930 940 950 960 SELAQEPRTK LPEMAASEHV IADYETTRLS LKGHPLQYLR EGLAAEGVST CRAVQEGADG 970 980 990 1000 1010 1020 RRMKVAGVVT VRQRPGSAKG VVFLTIEDET GIANIVIWPK IMKVFRREVM SARLIHIEGR 1030 1040 1050 1060 1070 IQRSLEGVVH LVAAKLQDRS AALIEMSGRE AQRLIAPSQM AHHPRNVRVM PNSRDFH

References

[1]

"Completion of the genome sequence of Brucella abortus and comparison to the highly similar genomes of Brucella melitensis and Brucella suis." Halling S.M., Peterson-Burch B.D., Bricker B.J., Zuerner R.L., Qing Z., Li L.-L., Kapur V., Alt D.P., Olsen S.C. J. Bacteriol. 187:2715-2726(2005) [PubMed: 15805518] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 9-941 / Biovar 1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017223 Genomic DNA. Translation: AAX73487.1.
RefSeq	YP_220848.1.
3D structure databases
SMR	Q57FU7. Positions 3-886.
ModBase	Search...
Genome annotation databases
GeneID	3339320.
GenomeReviews	Gene locus BruAb1_0069 in contig AE017223_GR.
KEGG	bmb:BruAb1_0069.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG734490.
OMA	MGFYSPD.
Enzyme and pathway databases
BioCyc	BABO262698:BRUAB1_0069-MONOMER.
BRENDA	2.7.7.7. 575.
Family and domain databases
HAMAP	MF_01902. DNApol_error_prone. [Tree]
InterPro	IPR011708. DNA_pol3_alpha. IPR004365. NA_bd_OB_tRNA-helicase. IPR004013. PHP_C. IPR003141. Pol/His_phosphatase_N. IPR016195. Pol/histidinol_Pase-like. IPR004805. PolC_alpha. [Graphical view]
Pfam	PF07733. DNA_pol3_alpha. 1 hit. PF02811. PHP. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
SMART	SM00481. POLIIIAc. 1 hit. [Graphical view]
TIGRFAMs	TIGR00594. polc. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DNAE2_BRUAB

Accession

Primary (citable) accession number: Q57FU7

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2005
Last sequence update:	May 10, 2005
Last modified:	February 9, 2010
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)