Q57FT9 (RLMN_BRUAB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribosomal RNA large subunit methyltransferase N
EC=2.1.1.192

Alternative name(s):
23S rRNA (adenine(2503)-C(2))-methyltransferase
23S rRNA m2A2503 methyltransferase

Gene names

Name:	rlmN
Ordered Locus Names:	BruAb1_0077

Organism

Brucella abortus biovar 1 (strain 9-941) [Complete proteome] [HAMAP]

Taxonomic identifier

262698 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	411 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Specifically methylates position 2 of adenine 2503 in 23S rRNA By similarity. HAMAP MF_01849
Catalytic activity	2 S-adenosyl-L-methionine + adenine(2503) in 23S rRNA = S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 2-methyladenine(2503) in 23S rRNA. HAMAP MF_01849
Cofactor	Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine Potential.
Subcellular location	Cytoplasm Potential HAMAP MF_01849.
Sequence similarities	Belongs to the radical SAM superfamily. RlmN family.

Ontologies

Keywords
Biological process	rRNA processing
Cellular component	Cytoplasm
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW rRNA (adenine-C2-)-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 411	411	Ribosomal RNA large subunit methyltransferase N HAMAP MF_01849		PRO_0000350062
Sites
	Metal binding	145	1	Iron-sulfur (4Fe-4S-S-AdoMet) Potential
	Metal binding	149	1	Iron-sulfur (4Fe-4S-S-AdoMet) Potential
	Metal binding	152	1	Iron-sulfur (4Fe-4S-S-AdoMet) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q57FT9 [UniParc]. Last modified May 10, 2005. Version 1. Checksum: 48D79A830A9A02BC	FASTA	411	45,813
	10 20 30 40 50 60 MSISFDLTID DTRDQLARHA RASLEAKPSL IGMSREEMAA ALIAAGVPER QVKMRISQLW 70 80 90 100 110 120 HWLYVRGVSD FADMRNISKD LRAMLAQHFT IARPEVVEEQ ISQDGTRKWL FRFPPRGAGR 130 140 150 160 170 180 PVEIESVYIP EEGRGTLCIS SQVGCTLTCS FCHTGTQKLV RNLTSEEILA QLLTARDRLG 190 200 210 220 230 240 DFPDKDTPDG AMVPAEGRKI TNIVMMGMGE PLYNFEEVKK ALLIASDGDG LSLSKCRITL 250 260 270 280 290 300 STSGVVPEIY RTGDEIGVML AISLHAVRDE LRDILVPINK KYPLAELIKA CREYPGLSNA 310 320 330 340 350 360 KRITFEYVML KDINDSLDDA KLLVKLLQGI PAKINLIPFN PWPGTNYQCS DWEQIEKFAD 370 380 390 400 410 YVNAAGYASP IRTPRGRDIL AACGQLKSES ERLRKSERLA LEAMMIAGHG E

References

[1]

"Completion of the genome sequence of Brucella abortus and comparison to the highly similar genomes of Brucella melitensis and Brucella suis." Halling S.M., Peterson-Burch B.D., Bricker B.J., Zuerner R.L., Qing Z., Li L.-L., Kapur V., Alt D.P., Olsen S.C. J. Bacteriol. 187:2715-2726(2005) [PubMed: 15805518] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 9-941.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017223 Genomic DNA. Translation: AAX73495.1.
RefSeq	YP_220856.1. NC_006932.1.
3D structure databases
ProteinModelPortal	Q57FT9.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3339179.
GenomeReviews	Gene locus BruAb1_0077 in contig AE017223_GR.
KEGG	bmb:BruAb1_0077.
PATRIC	17821675. VBIBruAbo15061_0082.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG335345.
OMA	PLYNFDA.
PhylomeDB	Q57FT9.
ProtClustDB	CLSK864022.
Enzyme and pathway databases
BioCyc	BABO262698:BRUAB1_0077-MONOMER.
Family and domain databases
HAMAP	MF_01849. 23SrRNA_methyltr_N. [Tree]
InterPro	IPR006638. Elp3/MiaB/NifB. IPR004383. rRNA_lsu_MTrfase_RlmN. IPR007197. rSAM. IPR023404. rSAM_horseshoe. [Graphical view]
Gene3D	G3DSA:3.80.30.20. G3DSA:3.80.30.20. 1 hit.
KO	K06941.
Pfam	PF04055. Radical_SAM. 1 hit. [Graphical view]
PIRSF	PIRSF006004. CHP00048. 1 hit.
SMART	SM00729. Elp3. 1 hit. [Graphical view]
TIGRFAMs	TIGR00048. TIGR00048. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RLMN_BRUAB

Accession

Primary (citable) accession number: Q57FT9

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	May 10, 2005
Last modified:	January 25, 2012
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program