Reviewed, UniProtKB/Swiss-Prot Q57FT9 (RLMN_BRUAB)

Names and origin

Protein names

Recommended name:
    Ribosomal RNA large subunit methyltransferase N
    EC=2.1.1.-
Alternative name(s):
    23S rRNA m2A2503 methyltransferase

Gene names

Name:	rlmN
Ordered Locus Names:	BruAb1_0077

Organism

Brucella abortus [Complete proteome] [HAMAP]

Taxonomic identifier

235 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	411 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Specifically methylates position 2 of adenine 2503 in 23S rRNA By similarity.
Cofactor	Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine Potential.
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the radical SAM superfamily. RlmN family.

Ontologies

Keywords
Biological process	rRNA processing
Cellular component	Cytoplasm
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	rRNA processing Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW RNA methyltransferase activity Inferred from electronic annotation. Source: InterPro S-adenosylmethionine-dependent methyltransferase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 411	411	Ribosomal RNA large subunit methyltransferase N HAMAP MF_01849		PRO_0000350062
Sites
	Metal binding	145	1	Iron-sulfur (4Fe-4S-S-AdoMet) Potential
	Metal binding	149	1	Iron-sulfur (4Fe-4S-S-AdoMet) Potential
	Metal binding	152	1	Iron-sulfur (4Fe-4S-S-AdoMet) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q57FT9-1 [UniParc]. Last modified May 10, 2005. Version 1. Checksum: 48D79A830A9A02BC	FASTA	411	45,813
	10 20 30 40 50 60 MSISFDLTID DTRDQLARHA RASLEAKPSL IGMSREEMAA ALIAAGVPER QVKMRISQLW 70 80 90 100 110 120 HWLYVRGVSD FADMRNISKD LRAMLAQHFT IARPEVVEEQ ISQDGTRKWL FRFPPRGAGR 130 140 150 160 170 180 PVEIESVYIP EEGRGTLCIS SQVGCTLTCS FCHTGTQKLV RNLTSEEILA QLLTARDRLG 190 200 210 220 230 240 DFPDKDTPDG AMVPAEGRKI TNIVMMGMGE PLYNFEEVKK ALLIASDGDG LSLSKCRITL 250 260 270 280 290 300 STSGVVPEIY RTGDEIGVML AISLHAVRDE LRDILVPINK KYPLAELIKA CREYPGLSNA 310 320 330 340 350 360 KRITFEYVML KDINDSLDDA KLLVKLLQGI PAKINLIPFN PWPGTNYQCS DWEQIEKFAD 370 380 390 400 410 YVNAAGYASP IRTPRGRDIL AACGQLKSES ERLRKSERLA LEAMMIAGHG E

References

[1]

"Completion of the genome sequence of Brucella abortus and comparison to the highly similar genomes of Brucella melitensis and Brucella suis." Halling S.M., Peterson-Burch B.D., Bricker B.J., Zuerner R.L., Qing Z., Li L.-L., Kapur V., Alt D.P., Olsen S.C. J. Bacteriol. 187:2715-2726(2005) [PubMed: 15805518] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 9-941 / Biovar 1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE017223 Genomic DNA. Translation: AAX73495.1.
RefSeq	YP_220856.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3339179.
GenomeReviews	Gene locus BruAb1_0077 in contig AE017223_GR.
KEGG	bmb:BruAb1_0077.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q57FT9.
OMA	TFCSTAQ
Enzyme and pathway databases
BioCyc	BABO262698:BRUAB1_0077-MON.
Family and domain databases
HAMAP	MF_01849. [Tree]
InterPro	IPR006638. Elp3/MiaB/NifB. IPR007197. Radical_SAM. IPR004383. rRNA_lsu_MTrfase_RlmN. [Graphical view]
Pfam	PF04055. Radical_SAM. 1 hit. [Graphical view]
PIRSF	PIRSF006004. CHP00048. 1 hit.
SMART	SM00729. Elp3. 1 hit. [Graphical view]
TIGRFAMs	TIGR00048. CHP48. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RLMN_BRUAB

Accession

Primary (citable) accession number: Q57FT9

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	May 10, 2005
Last modified:	November 24, 2009
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)