Reviewed, UniProtKB/Swiss-Prot Q57FK3 (RNPH_BRUAB)

Names and origin

Protein names

Recommended name:
    Ribonuclease PH
      Short name=RNase PH
    EC=2.7.7.56
Alternative name(s):
    tRNA nucleotidyltransferase

Gene names

Name:	rph
Ordered Locus Names:	BruAb1_0169

Organism

Brucella abortus [Complete proteome] [HAMAP]

Taxonomic identifier

235 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	238 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates By similarity. HAMAP MF_00564
Catalytic activity	tRNA(n+1) + phosphate = tRNA(n) + a nucleoside diphosphate. HAMAP MF_00564
Sequence similarities	Belongs to the RNase PH family.

Ontologies

Keywords
Biological process	tRNA processing
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA processing Inferred from electronic annotation. Source: HAMAP
Molecular function	3'-5'-exoribonuclease activity Inferred from electronic annotation. Source: InterPro tRNA binding Inferred from electronic annotation. Source: HAMAP tRNA nucleotidyltransferase activity Inferred from electronic annotation. Source: HAMAP tRNA-specific ribonuclease activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 238	238	Ribonuclease PH HAMAP MF_00564		PRO_1000024781

Sequences

	Sequence		Length	Mass (Da)
	Q57FK3-1 [UniParc]. Last modified May 10, 2005. Version 1. Checksum: 70CAF4B8F47A8E9C	FASTA	238	25,907
	10 20 30 40 50 60 MRPSKRAADE MRAISFERGV SKHAEGSCLV KFGDTHVLCT ASLEEKVPGW MRNTGKGWVT 70 80 90 100 110 120 AEYGMLPRST GERMRREAAA GKQGGRTQEI QRLIGRSLRA VVDMQALGEM QITVDCDVIQ 130 140 150 160 170 180 ADGGTRTAAI TGGWVALHEC LRWMEARQMV RVEKVLKDHV AAISCGIYEG VPVLDLDYAE 190 200 210 220 230 DSVAETDSNF VMTGKGGIVE IQGTAEGVPF SEEEFGALMK LARSGIDRLV SLQKMAVA

References

[1]

"Completion of the genome sequence of Brucella abortus and comparison to the highly similar genomes of Brucella melitensis and Brucella suis." Halling S.M., Peterson-Burch B.D., Bricker B.J., Zuerner R.L., Qing Z., Li L.-L., Kapur V., Alt D.P., Olsen S.C. J. Bacteriol. 187:2715-2726(2005) [PubMed: 15805518] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 9-941 / Biovar 1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017223 Genomic DNA. Translation: AAX73581.1.
RefSeq	YP_220942.1.
3D structure databases
HSSP	HSSP built from PDB template 1R6M based on UniProtKB P50597.
SMR	Q57FK3. Positions 2-238.
ModBase	Search...
Genome annotation databases
GeneID	3339146.
GenomeReviews	Gene locus BruAb1_0169 in contig AE017223_GR.
KEGG	bmb:BruAb1_0169.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG737187.
OMA	MLPRATG.
PhylomeDB	Q57FK3.
Enzyme and pathway databases
BioCyc	BABO262698:BRUAB1_0169-MONOMER.
BRENDA	2.7.7.56. 575.
Family and domain databases
HAMAP	MF_00564. RNase_PH. [Tree]
InterPro	IPR001247. ExoRNase_PH_dom1. IPR015847. ExoRNase_PH_dom2. IPR020568. Ribosomal_S5_D2-typ_fold. IPR002381. RNase_PH_bac-type. IPR018336. RNase_PH_CS. [Graphical view]
Pfam	PF01138. RNase_PH. 1 hit. PF03725. RNase_PH_C. 1 hit. [Graphical view]
TIGRFAMs	TIGR01966. RNasePH. 1 hit.
PROSITE	PS01277. RIBONUCLEASE_PH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RNPH_BRUAB

Accession

Primary (citable) accession number: Q57FK3

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	May 10, 2005
Last modified:	February 9, 2010
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)