Q57F97 (MURA_BRUAB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-N-acetylglucosamine 1-carboxyvinyltransferase
EC=2.5.1.7

Alternative name(s):
Enoylpyruvate transferase
UDP-N-acetylglucosamine enolpyruvyl transferase
Short name=EPT

Gene names

Name:	murA
Ordered Locus Names:	BruAb1_0282

Organism

Brucella abortus biovar 1 (strain 9-941) [Complete proteome] [HAMAP]

Taxonomic identifier

262698 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	429 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Cell wall formation. Adds enolpyruvyl to UDP-N-acetylglucosamine By similarity. HAMAP MF_00111
Catalytic activity	Phosphoenolpyruvate + UDP-N-acetyl-D-glucosamine = phosphate + UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine. HAMAP MF_00111
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00111
Subcellular location	Cytoplasm Probable HAMAP MF_00111.
Sequence similarities	Belongs to the EPSP synthase family. MurA subfamily.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	UDP-N-acetylgalactosamine biosynthetic process Inferred from electronic annotation. Source: InterPro cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	UDP-N-acetylglucosamine 1-carboxyvinyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 429	429	UDP-N-acetylglucosamine 1-carboxyvinyltransferase HAMAP MF_00111		PRO_0000231179
Sites
	Active site	126	1	Proton donor By similarity
Amino acid modifications
	Modified residue	126	1	2-(S-cysteinyl)pyruvic acid O-phosphothioketal By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q57F97 [UniParc]. Last modified May 10, 2005. Version 1. Checksum: 5F38AF6BFC895CA7	FASTA	429	45,719
	10 20 30 40 50 60 MDRIKIVGGN KLNGVIPISG AKNAALPLMI ASLLTDDTLT LENVPHLADV EQLIRILSNH 70 80 90 100 110 120 GVDYSVNGRR EHQNGPYSRT IHFTARNIVD TTAPYELVSR MRASFWVIGP LLARMGEANV 130 140 150 160 170 180 SLPGGCAIGT RPVDLLLDAL LALGAEIDIE NGYAKAKARN GLVGARYKFP KVSVGATHVM 190 200 210 220 230 240 LMAATLAKGE TIIENAAREP EVANLADCLN AMGAKISGAG SSTIHVQGVT NLSGARVRII 250 260 270 280 290 300 PDRIEAGTYA MAVAMTGGDV LLEGAQESQL SCVLETLRQA GAEINETNSG LRVVRNGHGI 310 320 330 340 350 360 QPVDITTDPF PGFPTDLQAQ FMGLMTRAKG TSHITETIFE NRFMHVQELA RLGAKISLSG 370 380 390 400 410 420 QTATVEGVER LKGAQVMATD LRASVSLVIA GLAAEGETIV NRVYHLDRGF ERLEEKLSRC GADVKRISG

References

[1]	"Completion of the genome sequence of Brucella abortus and comparison to the highly similar genomes of Brucella melitensis and Brucella suis." Halling S.M., Peterson-Burch B.D., Bricker B.J., Zuerner R.L., Qing Z., Li L.-L., Kapur V., Alt D.P., Olsen S.C. J. Bacteriol. 187:2715-2726(2005) [PubMed: 15805518] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 9-941.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017223 Genomic DNA. Translation: AAX73687.1.
RefSeq	YP_221048.1. NC_006932.1.
3D structure databases
ProteinModelPortal	Q57F97.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3338996.
GenomeReviews	Gene locus BruAb1_0282 in contig AE017223_GR.
KEGG	bmb:BruAb1_0282.
PATRIC	17822127. VBIBruAbo15061_0303.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG482701.
OMA	GYEHIED.
PhylomeDB	Q57F97.
ProtClustDB	PRK09369.
Enzyme and pathway databases
BioCyc	BABO262698:BRUAB1_0282-MONOMER.
Family and domain databases
HAMAP	MF_00111. MurA. [Tree]
InterPro	IPR001986. Enolpyruvate_Tfrase_dom. IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b. IPR005750. UDP_GlcNAc_COvinyl_MurA. [Graphical view]
Gene3D	G3DSA:3.65.10.10. EPSP_synthase. 2 hits.
KO	K00790.
PANTHER	PTHR21090:SF4. AcGlu_Tran_MurA. 1 hit.
Pfam	PF00275. EPSP_synthase. 1 hit. [Graphical view]
SUPFAM	SSF55205. RNA3'_cycl/enolpyr_transf_A/B. 1 hit.
TIGRFAMs	TIGR01072. MurA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURA_BRUAB

Accession

Primary (citable) accession number: Q57F97

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 4, 2006
Last sequence update:	May 10, 2005
Last modified:	January 25, 2012
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program