Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Ornithine carbamoyltransferase

Gene

argF

Organism
Brucella abortus biovar 1 (strain 9-941)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Reversibly catalyzes the transfer of the carbamoyl group from carbamoyl phosphate (CP) to the N(epsilon) atom of ornithine (ORN) to produce L-citrulline.By similarity

Catalytic activityi

Carbamoyl phosphate + L-ornithine = phosphate + L-citrulline.UniRule annotation

Pathway: L-arginine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate.UniRule annotation
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Ornithine carbamoyltransferase (argF)
  2. Argininosuccinate synthase (argG)
  3. Argininosuccinate lyase (argH)
This subpathway is part of the pathway L-arginine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate, the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei32 – 321Important for structural integrityUniRule annotation
Binding sitei84 – 841Carbamoyl phosphateUniRule annotation
Binding sitei108 – 1081Carbamoyl phosphateUniRule annotation
Sitei148 – 1481Important for structural integrityUniRule annotation
Binding sitei166 – 1661OrnithineBy similarity
Binding sitei226 – 2261OrnithineBy similarity
Binding sitei275 – 2751Carbamoyl phosphateUniRule annotation
Binding sitei293 – 2931Carbamoyl phosphateUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Arginine biosynthesis

Enzyme and pathway databases

BioCyciBABO262698:GJC2-334-MONOMER.
UniPathwayiUPA00068; UER00112.

Names & Taxonomyi

Protein namesi
Recommended name:
Ornithine carbamoyltransferaseUniRule annotation (EC:2.1.3.3UniRule annotation)
Short name:
OTCaseUniRule annotation
Gene namesi
Name:argFUniRule annotation
Ordered Locus Names:BruAb1_0328
OrganismiBrucella abortus biovar 1 (strain 9-941)
Taxonomic identifieri262698 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
ProteomesiUP000000540 Componenti: Chromosome I

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 312312Ornithine carbamoyltransferasePRO_1000065077Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliQ57F56.
SMRiQ57F56. Positions 8-307.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni57 – 615Carbamoyl phosphate bindingUniRule annotation
Regioni135 – 1384Carbamoyl phosphate bindingUniRule annotation
Regioni230 – 2312Ornithine bindingUniRule annotation
Regioni264 – 2674Carbamoyl phosphate bindingUniRule annotation

Sequence similaritiesi

Belongs to the ATCase/OTCase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0078.
HOGENOMiHOG000022686.
KOiK00611.
OMAiGNNVCNS.
OrthoDBiEOG690MGV.

Family and domain databases

Gene3Di3.40.50.1370. 2 hits.
HAMAPiMF_01109. OTCase.
InterProiIPR006132. Asp/Orn_carbamoyltranf_P-bd.
IPR006130. Asp/Orn_carbamoylTrfase.
IPR006131. Asp_carbamoyltransf_Asp/Orn-bd.
IPR002292. Orn/put_carbamltrans.
IPR024904. OTCase_ArgI.
[Graphical view]
PfamiPF00185. OTCace. 1 hit.
PF02729. OTCace_N. 1 hit.
[Graphical view]
PRINTSiPR00100. AOTCASE.
PR00102. OTCASE.
SUPFAMiSSF53671. SSF53671. 1 hit.
TIGRFAMsiTIGR00658. orni_carb_tr. 1 hit.
PROSITEiPS00097. CARBAMOYLTRANSFERASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q57F56-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MANDNGIKHF IDLSTVPATE LRAILEDAKA RKARLKAGEV ERPYAGKVLA
60 70 80 90 100
MIFEKLSTRT RVSFDVGMRQ LGGETIMLTG SEMQLGRSET IADTAKVLSR
110 120 130 140 150
YVDAIMIRTT AHERMLELAE YATVPVINAL TDDTHPCQIM ADVLTYEEHR
160 170 180 190 200
GPIKGKTFAW MGDGNNVLHS LVEAAARFDF NVNIATPKGS EPKSQYIDWA
210 220 230 240 250
RANGAGIMST TDPEKAASGA DCIVTDTWVS MGQEDHARGH NVFIPYQVNA
260 270 280 290 300
NLMAKADPKA LFMHCLPAHR GEEVTDEVID GPQSVVFDEA ENRLHAQKAI
310
LAWCLQDRGL GA
Length:312
Mass (Da):34,239
Last modified:May 10, 2005 - v1
Checksum:i60DBB4EC456B939C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017223 Genomic DNA. Translation: AAX73728.1.
RefSeqiWP_002963466.1. NC_006932.1.
YP_221089.1. NC_006932.1.

Genome annotation databases

EnsemblBacteriaiAAX73728; AAX73728; BruAb1_0328.
GeneIDi3787107.
KEGGibmb:BruAb1_0328.
PATRICi17822236. VBIBruAbo15061_0355.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017223 Genomic DNA. Translation: AAX73728.1.
RefSeqiWP_002963466.1. NC_006932.1.
YP_221089.1. NC_006932.1.

3D structure databases

ProteinModelPortaliQ57F56.
SMRiQ57F56. Positions 8-307.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAX73728; AAX73728; BruAb1_0328.
GeneIDi3787107.
KEGGibmb:BruAb1_0328.
PATRICi17822236. VBIBruAbo15061_0355.

Phylogenomic databases

eggNOGiCOG0078.
HOGENOMiHOG000022686.
KOiK00611.
OMAiGNNVCNS.
OrthoDBiEOG690MGV.

Enzyme and pathway databases

UniPathwayiUPA00068; UER00112.
BioCyciBABO262698:GJC2-334-MONOMER.

Family and domain databases

Gene3Di3.40.50.1370. 2 hits.
HAMAPiMF_01109. OTCase.
InterProiIPR006132. Asp/Orn_carbamoyltranf_P-bd.
IPR006130. Asp/Orn_carbamoylTrfase.
IPR006131. Asp_carbamoyltransf_Asp/Orn-bd.
IPR002292. Orn/put_carbamltrans.
IPR024904. OTCase_ArgI.
[Graphical view]
PfamiPF00185. OTCace. 1 hit.
PF02729. OTCace_N. 1 hit.
[Graphical view]
PRINTSiPR00100. AOTCASE.
PR00102. OTCASE.
SUPFAMiSSF53671. SSF53671. 1 hit.
TIGRFAMsiTIGR00658. orni_carb_tr. 1 hit.
PROSITEiPS00097. CARBAMOYLTRANSFERASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Completion of the genome sequence of Brucella abortus and comparison to the highly similar genomes of Brucella melitensis and Brucella suis."
    Halling S.M., Peterson-Burch B.D., Bricker B.J., Zuerner R.L., Qing Z., Li L.-L., Kapur V., Alt D.P., Olsen S.C.
    J. Bacteriol. 187:2715-2726(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 9-941.

Entry informationi

Entry nameiOTC_BRUAB
AccessioniPrimary (citable) accession number: Q57F56
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: May 10, 2005
Last modified: June 24, 2015
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Brucella abortus strain 9-941
    Brucella abortus (strain 9-941): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.