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Protein

Ornithine carbamoyltransferase

Gene

argF

Organism
Brucella abortus biovar 1 (strain 9-941)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Reversibly catalyzes the transfer of the carbamoyl group from carbamoyl phosphate (CP) to the N(epsilon) atom of ornithine (ORN) to produce L-citrulline.By similarity

Catalytic activityi

Carbamoyl phosphate + L-ornithine = phosphate + L-citrulline.UniRule annotation

Pathwayi: L-arginine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate.UniRule annotation
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Ornithine carbamoyltransferase (argF)
  2. Argininosuccinate synthase (argG)
  3. Argininosuccinate lyase (argH)
This subpathway is part of the pathway L-arginine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate, the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei84Carbamoyl phosphateUniRule annotation1
Binding sitei108Carbamoyl phosphateUniRule annotation1
Binding sitei166OrnithineBy similarity1
Binding sitei226OrnithineBy similarity1
Binding sitei275Carbamoyl phosphateUniRule annotation1
Binding sitei293Carbamoyl phosphateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Arginine biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00068; UER00112.

Names & Taxonomyi

Protein namesi
Recommended name:
Ornithine carbamoyltransferaseUniRule annotation (EC:2.1.3.3UniRule annotation)
Short name:
OTCaseUniRule annotation
Gene namesi
Name:argFUniRule annotation
Ordered Locus Names:BruAb1_0328
OrganismiBrucella abortus biovar 1 (strain 9-941)
Taxonomic identifieri262698 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
Proteomesi
  • UP000000540 Componenti: Chromosome I

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000650771 – 312Ornithine carbamoyltransferaseAdd BLAST312

Structurei

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei32Important for structural integrityUniRule annotation1
Sitei148Important for structural integrityUniRule annotation1

3D structure databases

ProteinModelPortaliQ57F56.
SMRiQ57F56.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni57 – 61Carbamoyl phosphate bindingUniRule annotation5
Regioni135 – 138Carbamoyl phosphate bindingUniRule annotation4
Regioni230 – 231Ornithine bindingUniRule annotation2
Regioni264 – 267Carbamoyl phosphate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the ATCase/OTCase family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000022686.
KOiK00611.
OMAiEHPCQIL.

Family and domain databases

Gene3Di3.40.50.1370. 2 hits.
HAMAPiMF_01109. OTCase. 1 hit.
InterProiIPR006132. Asp/Orn_carbamoyltranf_P-bd.
IPR006130. Asp/Orn_carbamoylTrfase.
IPR006131. Asp_carbamoyltransf_Asp/Orn-bd.
IPR002292. Orn/put_carbamltrans.
IPR024904. OTCase_ArgI.
[Graphical view]
PfamiPF00185. OTCace. 1 hit.
PF02729. OTCace_N. 1 hit.
[Graphical view]
PRINTSiPR00100. AOTCASE.
PR00102. OTCASE.
SUPFAMiSSF53671. SSF53671. 1 hit.
TIGRFAMsiTIGR00658. orni_carb_tr. 1 hit.
PROSITEiPS00097. CARBAMOYLTRANSFERASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q57F56-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MANDNGIKHF IDLSTVPATE LRAILEDAKA RKARLKAGEV ERPYAGKVLA
60 70 80 90 100
MIFEKLSTRT RVSFDVGMRQ LGGETIMLTG SEMQLGRSET IADTAKVLSR
110 120 130 140 150
YVDAIMIRTT AHERMLELAE YATVPVINAL TDDTHPCQIM ADVLTYEEHR
160 170 180 190 200
GPIKGKTFAW MGDGNNVLHS LVEAAARFDF NVNIATPKGS EPKSQYIDWA
210 220 230 240 250
RANGAGIMST TDPEKAASGA DCIVTDTWVS MGQEDHARGH NVFIPYQVNA
260 270 280 290 300
NLMAKADPKA LFMHCLPAHR GEEVTDEVID GPQSVVFDEA ENRLHAQKAI
310
LAWCLQDRGL GA
Length:312
Mass (Da):34,239
Last modified:May 10, 2005 - v1
Checksum:i60DBB4EC456B939C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017223 Genomic DNA. Translation: AAX73728.1.
RefSeqiWP_002963466.1. NC_006932.1.

Genome annotation databases

EnsemblBacteriaiAAX73728; AAX73728; BruAb1_0328.
GeneIDi3787107.
KEGGibmb:BruAb1_0328.
PATRICi17822236. VBIBruAbo15061_0355.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017223 Genomic DNA. Translation: AAX73728.1.
RefSeqiWP_002963466.1. NC_006932.1.

3D structure databases

ProteinModelPortaliQ57F56.
SMRiQ57F56.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAX73728; AAX73728; BruAb1_0328.
GeneIDi3787107.
KEGGibmb:BruAb1_0328.
PATRICi17822236. VBIBruAbo15061_0355.

Phylogenomic databases

HOGENOMiHOG000022686.
KOiK00611.
OMAiEHPCQIL.

Enzyme and pathway databases

UniPathwayiUPA00068; UER00112.

Family and domain databases

Gene3Di3.40.50.1370. 2 hits.
HAMAPiMF_01109. OTCase. 1 hit.
InterProiIPR006132. Asp/Orn_carbamoyltranf_P-bd.
IPR006130. Asp/Orn_carbamoylTrfase.
IPR006131. Asp_carbamoyltransf_Asp/Orn-bd.
IPR002292. Orn/put_carbamltrans.
IPR024904. OTCase_ArgI.
[Graphical view]
PfamiPF00185. OTCace. 1 hit.
PF02729. OTCace_N. 1 hit.
[Graphical view]
PRINTSiPR00100. AOTCASE.
PR00102. OTCASE.
SUPFAMiSSF53671. SSF53671. 1 hit.
TIGRFAMsiTIGR00658. orni_carb_tr. 1 hit.
PROSITEiPS00097. CARBAMOYLTRANSFERASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiOTC_BRUAB
AccessioniPrimary (citable) accession number: Q57F56
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: May 10, 2005
Last modified: November 2, 2016
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Brucella abortus strain 9-941
    Brucella abortus (strain 9-941): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.