Reviewed, UniProtKB/Swiss-Prot Q57EX6 (RNH2_BRUAB)

Names and origin

Protein names

Recommended name:
    Ribonuclease HII
      Short name=RNase HII
    EC=3.1.26.4

Gene names

Name:	rnhB
Ordered Locus Names:	BruAb1_0411

Organism

Brucella abortus [Complete proteome] [HAMAP]

Taxonomic identifier

235 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	220 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Endonuclease that specifically degrades the RNA of RNA-DNA hybrids By similarity.
Catalytic activity	Endonucleolytic cleavage to 5'-phosphomonoester. HAMAP MF_00052
Cofactor	Manganese or magnesium. Binds 1 divalent metal ion per monomer in the absence of substrate. May bind a second metal ion after substrate binding By similarity.
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the RNase HII family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Manganese Metal-binding
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	RNA catabolic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	RNA binding Inferred from electronic annotation. Source: InterPro manganese ion binding Inferred from electronic annotation. Source: HAMAP ribonuclease H activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 220	220	Ribonuclease HII HAMAP MF_00052		PRO_0000111550
Sites
	Metal binding	38	1	Divalent metal cation By similarity
	Metal binding	39	1	Divalent metal cation By similarity
	Metal binding	130	1	Divalent metal cation By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q57EX6-1 [UniParc]. Last modified May 10, 2005. Version 1. Checksum: 87E3AB1CD4844E8E	FASTA	220	23,332
	10 20 30 40 50 60 MKRSASDSPL LFDLPLAPDF SQEQQLMKRG LKHIAGIDEA GRGPLAGPVV AAAVVLDQND 70 80 90 100 110 120 LPEGLDDSKR LTAARREALY EIILTKAITV SVASLSARSI DASDIRKAAL EAMRRAVIGL 130 140 150 160 170 180 TLKPCHALVD GRDVPPGLPC PGSALVKGDQ RSVSIAAASI VAKVTRDRMM IRAGAAHPPY 190 200 210 220 GLEIHAGYAT QKHRAAIESE GPVPGLHRYT FAPIKGCFDC

References

[1]

"Completion of the genome sequence of Brucella abortus and comparison to the highly similar genomes of Brucella melitensis and Brucella suis." Halling S.M., Peterson-Burch B.D., Bricker B.J., Zuerner R.L., Qing Z., Li L.-L., Kapur V., Alt D.P., Olsen S.C. J. Bacteriol. 187:2715-2726(2005) [PubMed: 15805518] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 9-941 / Biovar 1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE017223 Genomic DNA. Translation: AAX73808.1.
RefSeq	YP_221169.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3339134.
GenomeReviews	Gene locus BruAb1_0411 in contig AE017223_GR.
KEGG	bmb:BruAb1_0411.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q57EX6.
OMA	RLGPTPI
Enzyme and pathway databases
BioCyc	BABO262698:BRUAB1_0411-MON.
BRENDA	3.1.26.4. 575.
Family and domain databases
HAMAP	MF_00052. [Tree]
InterPro	IPR012337. PolynucTfrase_RNaseH_fold. IPR001352. RNase_HII/HIII. [Graphical view]
PANTHER	PTHR10954. RNase_HII/HIII. 1 hit.
Pfam	PF01351. RNase_HII. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RNH2_BRUAB

Accession

Primary (citable) accession number: Q57EX6

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2005
Last sequence update:	May 10, 2005
Last modified:	November 24, 2009
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)