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Protein

Ribonuclease HII

Gene

rnhB

Organism
Brucella abortus biovar 1 (strain 9-941)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Endonuclease that specifically degrades the RNA of RNA-DNA hybrids.UniRule annotation

Catalytic activityi

Endonucleolytic cleavage to 5'-phosphomonoester.UniRule annotation

Cofactori

Mn2+UniRule annotation, Mg2+UniRule annotationNote: Manganese or magnesium. Binds 1 divalent metal ion per monomer in the absence of substrate. May bind a second metal ion after substrate binding.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi38 – 381Divalent metal cationUniRule annotation
Metal bindingi39 – 391Divalent metal cationUniRule annotation
Metal bindingi130 – 1301Divalent metal cationUniRule annotation

GO - Molecular functioni

  1. manganese ion binding Source: UniProtKB-HAMAP
  2. RNA binding Source: InterPro
  3. RNA-DNA hybrid ribonuclease activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. RNA catabolic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

BioCyciBABO262698:GJC2-417-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonuclease HIIUniRule annotation (EC:3.1.26.4UniRule annotation)
Short name:
RNase HIIUniRule annotation
Gene namesi
Name:rnhBUniRule annotation
Ordered Locus Names:BruAb1_0411
OrganismiBrucella abortus biovar 1 (strain 9-941)
Taxonomic identifieri262698 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
ProteomesiUP000000540 Componenti: Chromosome I

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 220220Ribonuclease HIIPRO_0000111550Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi262698.BruAb1_0411.

Family & Domainsi

Sequence similaritiesi

Belongs to the RNase HII family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0164.
HOGENOMiHOG000100288.
KOiK03470.
OMAiKIQPHFV.
OrthoDBiEOG6BKJC8.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
HAMAPiMF_00052_B. RNase_HII_B.
InterProiIPR022898. RNase_HII.
IPR001352. RNase_HII/HIII.
IPR024567. RNase_HII/HIII_dom.
IPR012337. RNaseH-like_dom.
[Graphical view]
PANTHERiPTHR10954. PTHR10954. 1 hit.
PfamiPF01351. RNase_HII. 1 hit.
[Graphical view]
SUPFAMiSSF53098. SSF53098. 1 hit.

Sequencei

Sequence statusi: Complete.

Q57EX6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKRSASDSPL LFDLPLAPDF SQEQQLMKRG LKHIAGIDEA GRGPLAGPVV
60 70 80 90 100
AAAVVLDQND LPEGLDDSKR LTAARREALY EIILTKAITV SVASLSARSI
110 120 130 140 150
DASDIRKAAL EAMRRAVIGL TLKPCHALVD GRDVPPGLPC PGSALVKGDQ
160 170 180 190 200
RSVSIAAASI VAKVTRDRMM IRAGAAHPPY GLEIHAGYAT QKHRAAIESE
210 220
GPVPGLHRYT FAPIKGCFDC
Length:220
Mass (Da):23,332
Last modified:May 10, 2005 - v1
Checksum:i87E3AB1CD4844E8E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017223 Genomic DNA. Translation: AAX73808.1.
RefSeqiYP_221169.1. NC_006932.1.

Genome annotation databases

EnsemblBacteriaiAAX73808; AAX73808; BruAb1_0411.
KEGGibmb:BruAb1_0411.
PATRICi17822404. VBIBruAbo15061_0439.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017223 Genomic DNA. Translation: AAX73808.1.
RefSeqiYP_221169.1. NC_006932.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi262698.BruAb1_0411.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAX73808; AAX73808; BruAb1_0411.
KEGGibmb:BruAb1_0411.
PATRICi17822404. VBIBruAbo15061_0439.

Phylogenomic databases

eggNOGiCOG0164.
HOGENOMiHOG000100288.
KOiK03470.
OMAiKIQPHFV.
OrthoDBiEOG6BKJC8.

Enzyme and pathway databases

BioCyciBABO262698:GJC2-417-MONOMER.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
HAMAPiMF_00052_B. RNase_HII_B.
InterProiIPR022898. RNase_HII.
IPR001352. RNase_HII/HIII.
IPR024567. RNase_HII/HIII_dom.
IPR012337. RNaseH-like_dom.
[Graphical view]
PANTHERiPTHR10954. PTHR10954. 1 hit.
PfamiPF01351. RNase_HII. 1 hit.
[Graphical view]
SUPFAMiSSF53098. SSF53098. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Completion of the genome sequence of Brucella abortus and comparison to the highly similar genomes of Brucella melitensis and Brucella suis."
    Halling S.M., Peterson-Burch B.D., Bricker B.J., Zuerner R.L., Qing Z., Li L.-L., Kapur V., Alt D.P., Olsen S.C.
    J. Bacteriol. 187:2715-2726(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 9-941.

Entry informationi

Entry nameiRNH2_BRUAB
AccessioniPrimary (citable) accession number: Q57EX6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: May 10, 2005
Last modified: April 1, 2015
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Brucella abortus strain 9-941
    Brucella abortus (strain 9-941): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.