Q57EW2 (SYGA_BRUAB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycine--tRNA ligase alpha subunit
EC=6.1.1.14

Alternative name(s):
Glycyl-tRNA synthetase alpha subunit
Short name=GlyRS

Gene names

Name:	glyQ
Ordered Locus Names:	BruAb1_0426

Organism

Brucella abortus biovar 1 (strain 9-941) [Complete proteome] [HAMAP]

Taxonomic identifier

262698 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	308 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-tRNA(Gly). HAMAP MF_00254
Subunit structure	Tetramer of two alpha and two beta subunits By similarity.
Subcellular location	Cytoplasm HAMAP MF_00254.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glycine-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 308	308	Glycine--tRNA ligase alpha subunit HAMAP MF_00254		PRO_1000047403

Sequences

	Sequence		Length	Mass (Da)
	Q57EW2 [UniParc]. Last modified May 10, 2005. Version 1. Checksum: 6DD60DEDF2F376A7	FASTA	308	34,945
	10 20 30 40 50 60 MHPTRSFQGL ILTLHNYWAE HGCAILQPYD MEVGAGTFHP ATTLRSLGPK PWKAAYVQPS 70 80 90 100 110 120 RRPKDGRYGE NPNRLQHYYQ YQVLIKPSPP NLQDLYLGSL KAIGLDPTLH DVRFVEDDWE 130 140 150 160 170 180 SPTLGAWGLG WECWCDGMEV SQFTYFQQVC GIECSPVAGE LTYGLERLAM YVQGVDNVYD 190 200 210 220 230 240 LNFNGLEGDE KVTYGDVFLQ AEQEYSRYNF EMANTETLHQ HFIDAERECE AILKAGSTGE 250 260 270 280 290 300 NSLHKCVFPA YDQCIKASHV FNLMDARGVI SVTERQGYIL RVRNLARQCG EAFLLTDAGG FNFKREGE

References

[1]

"Completion of the genome sequence of Brucella abortus and comparison to the highly similar genomes of Brucella melitensis and Brucella suis." Halling S.M., Peterson-Burch B.D., Bricker B.J., Zuerner R.L., Qing Z., Li L.-L., Kapur V., Alt D.P., Olsen S.C. J. Bacteriol. 187:2715-2726(2005) [PubMed: 15805518] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 9-941.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017223 Genomic DNA. Translation: AAX73822.1.
RefSeq	YP_221183.1. NC_006932.1.
3D structure databases
HSSP	HSSP built from PDB template 1J5W based on UniProtKB Q9WY59.
ProteinModelPortal	Q57EW2.
SMR	Q57EW2. Positions 6-297.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3340903.
GenomeReviews	Gene locus BruAb1_0426 in contig AE017223_GR.
KEGG	bmb:BruAb1_0426.
PATRIC	17822434. VBIBruAbo15061_0454.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG285316.
OMA	CRRPTDG.
PhylomeDB	Q57EW2.
ProtClustDB	PRK09348.
Enzyme and pathway databases
BioCyc	BABO262698:BRUAB1_0426-MONOMER.
Family and domain databases
HAMAP	MF_00254. Gly_tRNA_synth_alpha. [Tree]
InterPro	IPR006194. Gly-tRNA-synth_II_heterodimer. IPR002310. Gly-tRNA_synth_IIc_asu. [Graphical view]
KO	K01878.
Pfam	PF02091. tRNA-synt_2e. 1 hit. [Graphical view]
PRINTS	PR01044. TRNASYNTHGA.
TIGRFAMs	TIGR00388. GlyQ. 1 hit.
PROSITE	PS50861. AA_TRNA_LIGASE_II_GLYAB. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYGA_BRUAB

Accession

Primary (citable) accession number: Q57EW2

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	May 10, 2005
Last modified:	January 25, 2012
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program