Q57ET1 (DXS_BRUAB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
1-deoxy-D-xylulose-5-phosphate synthase
EC=2.2.1.7

Alternative name(s):
1-deoxyxylulose-5-phosphate synthase
Short name=DXP synthase
Short name=DXPS

Gene names

Name:	dxs
Ordered Locus Names:	BruAb1_0458

Organism

Brucella abortus biovar 1 (strain 9-941) [Complete proteome] [HAMAP]

Taxonomic identifier

262698 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	643 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the acyloin condensation reaction between C atoms 2 and 3 of pyruvate and glyceraldehyde 3-phosphate to yield 1-deoxy-D-xylulose-5-phosphate (DXP) By similarity. HAMAP MF_00315
Catalytic activity	Pyruvate + D-glyceraldehyde 3-phosphate = 1-deoxy-D-xylulose 5-phosphate + CO2. HAMAP MF_00315
Cofactor	Binds 1 thiamine pyrophosphate per subunit By similarity.
Pathway	Metabolic intermediate biosynthesis; 1-deoxy-D-xylulose 5-phosphate biosynthesis; 1-deoxy-D-xylulose 5-phosphate from D-glyceraldehyde 3-phosphate and pyruvate: step 1/1. HAMAP MF_00315
Subunit structure	Homodimer By similarity. HAMAP MF_00315
Sequence similarities	Belongs to the transketolase family. DXPS subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis Thiamine biosynthesis
Ligand	Thiamine pyrophosphate
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: InterPro thiamine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	1-deoxy-D-xylulose-5-phosphate synthase activity Inferred from electronic annotation. Source: EC oxidoreductase activity, acting on the aldehyde or oxo group of donors, disulfide as acceptor Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 643	643	1-deoxy-D-xylulose-5-phosphate synthase HAMAP MF_00315		PRO_0000256386

Sequences

	Sequence		Length	Mass (Da)
	Q57ET1 [UniParc]. Last modified May 10, 2005. Version 1. Checksum: 869F89EEEA97534D	FASTA	643	69,212
	10 20 30 40 50 60 MSRPSTPLLD KAPTPDRLRA LPEQDLPQLA EELRTELIDA VSTTGGHLGA GLGVVELTVA 70 80 90 100 110 120 LHHVFNTPYD RIIWDVGHQA YPHKILTGRR DRIRTLRQAG GLSGFTKRAE SEYDPFGAAH 130 140 150 160 170 180 SSTSISAGLG MAVASELSGE KRNVIAVIGD GSMSAGMAYE AMNNAGALDA RLIVILNDND 190 200 210 220 230 240 MSIAPPTGAM SAYLARLVSG RTYRSVREAA KQVAQKLPKF LQDKARKSEE YARAFFTGGT 250 260 270 280 290 300 LFEELGFYYV GPIDGHNLDH LLPVLKNVRD TQKGPVLIHV VTQKGKGYAP AEAAADKYHG 310 320 330 340 350 360 VNKFDVITGK QAKPPANAPS YTKIFGTSLI EEARHDDKIV AVTAAMPTGT GLDLFGEAFP 370 380 390 400 410 420 KRVFDVGIAE QHAVTFAAGL ASEGYKPFCA IYSTFLQRGY DQVVHDVSIQ NLPVRFPIDR 430 440 450 460 470 480 AGLVGADGPT HAGSFDTGFL AALPGFVVMA ASDEAELRHM VRTAAEYDEG PISFRYPRGD 490 500 510 520 530 540 GVGVDLPERG SVLEIGKGRI VREGTKVALL SFGTRLQECL AAAEELGAAG LSTTVADARF 550 560 570 580 590 600 AKPLDHDLIR RLAREHEVLV MVEEGAVGGF SSHVLQFLAT DGLLDRGLKV RALMLPDIYQ 610 620 630 640 DHGKPDAMYA EAGLDRTGIV RTVFAALHRD ELGHEALPTP FRA

References

[1]

"Completion of the genome sequence of Brucella abortus and comparison to the highly similar genomes of Brucella melitensis and Brucella suis." Halling S.M., Peterson-Burch B.D., Bricker B.J., Zuerner R.L., Qing Z., Li L.-L., Kapur V., Alt D.P., Olsen S.C. J. Bacteriol. 187:2715-2726(2005) [PubMed: 15805518] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 9-941.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017223 Genomic DNA. Translation: AAX73853.1.
RefSeq	YP_221214.1. NC_006932.1.
3D structure databases
ProteinModelPortal	Q57ET1.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3339001.
GenomeReviews	Gene locus BruAb1_0458 in contig AE017223_GR.
KEGG	bmb:BruAb1_0458.
PATRIC	17822498. VBIBruAbo15061_0486.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG571647.
OMA	DPILYHG.
ProtClustDB	PRK05444.
Enzyme and pathway databases
BioCyc	BABO262698:BRUAB1_0458-MONOMER.
Family and domain databases
HAMAP	MF_00315. DXP_synth. [Tree]
InterPro	IPR001017. DH_E1. IPR005477. Dxylulose-5-P_synthase. IPR009014. Transketo_C/Pyr-ferredox_oxred. IPR015941. Transketolase-like_C. IPR005475. Transketolase-like_Pyr-bd. IPR020826. Transketolase_BS. IPR005476. Transketolase_C. IPR005474. Transketolase_N. [Graphical view]
Gene3D	G3DSA:3.40.50.920. Transketo_C_like. 1 hit.
KO	K01662.
Pfam	PF00676. E1_dh. 1 hit. PF02779. Transket_pyr. 1 hit. PF02780. Transketolase_C. 1 hit. [Graphical view]
SMART	SM00861. Transket_pyr. 1 hit. [Graphical view]
SUPFAM	SSF52922. Transketo_C_like. 1 hit.
TIGRFAMs	TIGR00204. Dxs. 1 hit.
PROSITE	PS00801. TRANSKETOLASE_1. 1 hit. PS00802. TRANSKETOLASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DXS_BRUAB

Accession

Primary (citable) accession number: Q57ET1

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2006
Last sequence update:	May 10, 2005
Last modified:	January 25, 2012
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program