Reviewed, UniProtKB/Swiss-Prot Q57EQ3 (KGUA_BRUAB)

Names and origin

Protein names

Recommended name:
    Guanylate kinase
    EC=2.7.4.8
Alternative name(s):
    GMP kinase

Gene names

Name:	gmk
Ordered Locus Names:	BruAb1_0486

Organism

Brucella abortus [Complete proteome] [HAMAP]

Taxonomic identifier

235 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	220 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Essential for recycling GMP and indirectly, cGMP By similarity. HAMAP MF_00328
Catalytic activity	ATP + GMP = ADP + GDP. HAMAP MF_00328
Subcellular location	Cytoplasm By similarity HAMAP MF_00328.
Sequence similarities	Belongs to the guanylate kinase family. Contains 1 guanylate kinase-like domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	purine nucleotide metabolic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP guanylate kinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 220	220	Guanylate kinase HAMAP MF_00328		PRO_0000266293
Regions
	Domain	14 – 194	181	Guanylate kinase-like
	Nucleotide binding	21 – 28	8	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q57EQ3-1 [UniParc]. Last modified May 10, 2005. Version 1. Checksum: C21405890D05491F	FASTA	220	25,223
	10 20 30 40 50 60 MAISSVENGV ARRGLMVVIS SPSGAGKSTI ARLLLEDPKM KLSLSISVTT RQRRPSEIDG 70 80 90 100 110 120 VHYHFITIRE FERLRDNDEL IEWAEVHGNF YGTLRETAEI ALADGQDMLF DIDWQGADQL 130 140 150 160 170 180 QAKMPADVVS IFILPPTMRE LQQRLNRRAE DTAEVIETRL QNARFEIQKW VKYDYIVINE 190 200 210 220 DLDRSYAAIK SIINAERLRR DRRPGLFDFV TGLLEEDPGM

References

[1]

"Completion of the genome sequence of Brucella abortus and comparison to the highly similar genomes of Brucella melitensis and Brucella suis." Halling S.M., Peterson-Burch B.D., Bricker B.J., Zuerner R.L., Qing Z., Li L.-L., Kapur V., Alt D.P., Olsen S.C. J. Bacteriol. 187:2715-2726(2005) [PubMed: 15805518] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 9-941 / Biovar 1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017223 Genomic DNA. Translation: AAX73881.1.
RefSeq	YP_221242.1.
3D structure databases
HSSP	HSSP built from PDB template 1S96 based on UniProtKB P60546.
SMR	Q57EQ3. Positions 15-194.
ModBase	Search...
Genome annotation databases
GeneID	3339389.
GenomeReviews	Gene locus BruAb1_0486 in contig AE017223_GR.
KEGG	bmb:BruAb1_0486.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG671982.
OMA	DIDWQGT.
PhylomeDB	Q57EQ3.
Enzyme and pathway databases
BioCyc	BABO262698:BRUAB1_0486-MONOMER.
BRENDA	2.7.4.8. 575.
Family and domain databases
HAMAP	MF_00328. Guanylate_kinase. [Tree]
InterPro	IPR008144. Guanylate_kin. IPR008145. Guanylate_kin/L-typ_Ca_channel. IPR020590. Guanylate_kinase_CS. IPR017665. Guanylate_kinase_sub. [Graphical view]
Pfam	PF00625. Guanylate_kin. 1 hit. [Graphical view]
SMART	SM00072. GuKc. 1 hit. [Graphical view]
TIGRFAMs	TIGR03263. guanyl_kin. 1 hit.
PROSITE	PS00856. GUANYLATE_KINASE_1. 1 hit. PS50052. GUANYLATE_KINASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KGUA_BRUAB

Accession

Primary (citable) accession number: Q57EQ3

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 12, 2006
Last sequence update:	May 10, 2005
Last modified:	February 9, 2010
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)