Q57EP6 (ISPH_BRUAB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
4-hydroxy-3-methylbut-2-enyl diphosphate reductase
EC=1.17.1.2

Gene names

Name:	ispH
Synonyms:	lytB
Ordered Locus Names:	BruAb1_0497

Organism

Brucella abortus biovar 1 (strain 9-941) [Complete proteome] [HAMAP]

Taxonomic identifier

262698 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	346 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) By similarity. HAMAP MF_00191
Catalytic activity	Isopentenyl diphosphate + NAD(P)+ + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H. HAMAP MF_00191 Dimethylallyl diphosphate + NAD(P)+ + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H. HAMAP MF_00191
Cofactor	Binds 1 3Fe-4S cluster By similarity. HAMAP MF_00191
Pathway	Isoprenoid biosynthesis; dimethylallyl diphosphate biosynthesis; dimethylallyl diphosphate from (2E)-4-hydroxy-3-methylbutenyl diphosphate: step 1/1. HAMAP MF_00191 Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 6/6. HAMAP MF_00191
Sequence similarities	Belongs to the IspH family.
Sequence caution	The sequence AAX73888.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	3Fe-4S Iron Iron-sulfur Metal-binding NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isopentenyl diphosphate biosynthetic process, mevalonate-independent pathway Inferred from electronic annotation. Source: InterPro
Molecular function	3 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 4-hydroxy-3-methylbut-2-en-1-yl diphosphate reductase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 346	346	4-hydroxy-3-methylbut-2-enyl diphosphate reductase HAMAP MF_00191		PRO_0000128786

Sequences

	Sequence		Length	Mass (Da)
	Q57EP6 [UniParc]. Last modified July 19, 2005. Version 2. Checksum: 151FA656894DABD4	FASTA	346	37,738
	10 20 30 40 50 60 MTQQRPPLEI RLCGPRGFCA GVDRAIQIVV LALKKYGAPV YVRHEIVHNR YVVEGLQARG 70 80 90 100 110 120 AIFVEELDEI PAAHRNQPVV FSAHGVPKSV PADAEAKNLF YLDATCPLVS KVHKQAMRHQ 130 140 150 160 170 180 RLGRHVILIG HSGHPEVIGT MGQLPDGAVT LIETVEDAHT CHFDDEDNLG FVTQTTLSVD 190 200 210 220 230 240 DTAGIIKELQ ARFPNLAAPA AESICYATTN RQDAVRAAAP GCDLFLIVGA PNSSNSKRLV 250 260 270 280 290 300 EVAEKAGARM SMLVQRAEDI EWEQIGDISV VGLSAGASAP EIIVDEIIDA FKARFDVKIE 310 320 330 340 LAETTVETEN FLVNREIRDV ELTVKDMAFV NGEHRVVGIS KLMQGK

References

[1]

"Completion of the genome sequence of Brucella abortus and comparison to the highly similar genomes of Brucella melitensis and Brucella suis." Halling S.M., Peterson-Burch B.D., Bricker B.J., Zuerner R.L., Qing Z., Li L.-L., Kapur V., Alt D.P., Olsen S.C. J. Bacteriol. 187:2715-2726(2005) [PubMed: 15805518] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 9-941.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017223 Genomic DNA. Translation: AAX73888.1. Different initiation.
RefSeq	YP_221249.1. NC_006932.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3339019.
GenomeReviews	Gene locus BruAb1_0497 in contig AE017223_GR.
KEGG	bmb:BruAb1_0497.
PATRIC	17822578. VBIBruAbo15061_0526.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG335228.
ProtClustDB	PRK01045.
Enzyme and pathway databases
BioCyc	BABO262698:BRUAB1_0497-MONOMER.
Family and domain databases
HAMAP	MF_00191. IspH. [Tree]
InterPro	IPR003451. LytB. [Graphical view]
KO	K03527.
Pfam	PF02401. LYTB. 1 hit. [Graphical view]
TIGRFAMs	TIGR00216. IspH_lytB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ISPH_BRUAB

Accession

Primary (citable) accession number: Q57EP6

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2005
Last sequence update:	July 19, 2005
Last modified:	January 25, 2012
This is version 44 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program