Q57EP4 (RNH_BRUAB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribonuclease H
Short name=RNase H
EC=3.1.26.4

Gene names

Name:	rnhA
Ordered Locus Names:	BruAb1_0499

Organism

Brucella abortus biovar 1 (strain 9-941) [Complete proteome] [HAMAP]

Taxonomic identifier

262698 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	154 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Endonuclease that specifically degrades the RNA of RNA-DNA hybrids By similarity. HAMAP MF_00042
Catalytic activity	Endonucleolytic cleavage to 5'-phosphomonoester. HAMAP MF_00042
Cofactor	Binds 1 magnesium ion per subunit. May bind a second metal ion at a regulatory site, or after substrate binding By similarity.
Subunit structure	Monomer By similarity. HAMAP MF_00042
Subcellular location	Cytoplasm Potential HAMAP MF_00042.
Sequence similarities	Belongs to the RNase H family. Contains 1 RNase H domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Magnesium Metal-binding
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleic acid binding Inferred from electronic annotation. Source: InterPro ribonuclease H activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 154	154	Ribonuclease H HAMAP MF_00042		PRO_0000195364
Regions
	Domain	1 – 141	141	RNase H
Sites
	Metal binding	9	1	Magnesium 1 By similarity
	Metal binding	9	1	Magnesium 2 By similarity
	Metal binding	47	1	Magnesium 1 By similarity
	Metal binding	69	1	Magnesium 1 By similarity
	Metal binding	133	1	Magnesium 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q57EP4 [UniParc]. Last modified May 10, 2005. Version 1. Checksum: 3B76C03F52E01012	FASTA	154	17,172
	10 20 30 40 50 60 MKRIEAYTDG ACSGNPGPGG WGALLRWNGN EKELKGGEAE TTNNRMELMA AISALSALKE 70 80 90 100 110 120 PCEVDLYTDS VYVRDGISGW IEGWKRNGWK TAAKKPVKNA ELWQALDEAR KAHKVTWHWI 130 140 150 KGHAGHPENE RADELARAGM EPFKYAGHRT LKVK

References

[1]	"Completion of the genome sequence of Brucella abortus and comparison to the highly similar genomes of Brucella melitensis and Brucella suis." Halling S.M., Peterson-Burch B.D., Bricker B.J., Zuerner R.L., Qing Z., Li L.-L., Kapur V., Alt D.P., Olsen S.C. J. Bacteriol. 187:2715-2726(2005) [PubMed: 15805518] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 9-941.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017223 Genomic DNA. Translation: AAX73890.1.
RefSeq	YP_221251.1. NC_006932.1.
3D structure databases
ProteinModelPortal	Q57EP4.
SMR	Q57EP4. Positions 1-139.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3340347.
GenomeReviews	Gene locus BruAb1_0499 in contig AE017223_GR.
KEGG	bmb:BruAb1_0499.
PATRIC	17822582. VBIBruAbo15061_0528.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG742437.
OMA	NGWRTAD.
PhylomeDB	Q57EP4.
ProtClustDB	PRK00203.
Enzyme and pathway databases
BioCyc	BABO262698:BRUAB1_0499-MONOMER.
Family and domain databases
HAMAP	MF_00042. RNase_H. [Tree]
InterPro	IPR022892. RNaseH. IPR012337. RNaseH-like_dom. IPR002156. RNaseH_domain. [Graphical view]
KO	K03469.
Pfam	PF00075. RNase_H. 1 hit. [Graphical view]
SUPFAM	SSF53098. RNaseH_fold. 1 hit.
PROSITE	PS50879. RNASE_H. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RNH_BRUAB

Accession

Primary (citable) accession number: Q57EP4

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 13, 2005
Last sequence update:	May 10, 2005
Last modified:	January 25, 2012
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program