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Q57E90 (RSH_BRUAB) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 69. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
GTP pyrophosphokinase rsh

EC=2.7.6.5
Alternative name(s):
(p)ppGpp synthase
ATP:GTP 3'-pyrophosphotransferase
Gene names
Name:rsh
Synonyms:spoT
Ordered Locus Names:BruAb1_0669
OrganismBrucella abortus biovar 1 (strain 9-941) [Complete proteome] [HAMAP]
Taxonomic identifier262698 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella

Protein attributes

Sequence length750 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Functions as a (p)ppGpp synthase. In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. Plays a role in adaptation of Brucella to its intracellular host environment. Ref.2

Catalytic activity

ATP + GTP = AMP + guanosine 3'-diphosphate 5'-triphosphate.

Disruption phenotype

Cells show morphological abnormalities such as branching and swelling forms. It does not grow in RPMI-1640 with or without 10% FBS. It shows decreased growth until 48 hours in RPMI-1640 and stationary growth until 48 hours in RPMI-1640 with 10% FBS. It has the same ability as the wild-type to internalize at 0 minute but a lower rate of internalization at 30 minutes. It shows a lower rate of intracellular replication within macrophages than the wild-type. Its adherence is four times higher than that of the wild-type. It shows lower MICs than the wild-type for several antimicrobial agents such as penicillin G, methicillin, erythromycin and doxycycline. At ten days post infection the number of viable bacteria (disruption mutant) is markedly reduced compared to the wild-type. Ref.2

Sequence similarities

Belongs to the RelA/SpoT family.

Contains 1 ACT domain.

Contains 1 HD domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 750750GTP pyrophosphokinase rsh
PRO_0000322563

Regions

Domain45 – 144100HD
Domain676 – 75075ACT

Sequences

Sequence LengthMass (Da)Tools
Q57E90 [UniParc].

Last modified May 10, 2005. Version 1.
Checksum: 4836EEECB0E52495

FASTA75083,856
        10         20         30         40         50         60 
MMRQYELVER VQRYKPDVNE ALLNKAYVYA MQKHGSQKRA SGDPYFSHPL EVAAILTDMH 

        70         80         90        100        110        120 
LDEATIAIAL LHDTIEDTTA TRQEIDQLFG PEIGKLVEGL TKLKKLDLVS KKAVQAENLR 

       130        140        150        160        170        180 
KLLLAISEDV RVLLVKLADR LHNMRTLGVM REDKRLRIAE ETMDIYAPLA GRMGMQDMRE 

       190        200        210        220        230        240 
ELEELAFRYI NPDAWRAVTD RLAELLEKNR GLLQKIETDL SEIFEKNGIK ASVKSRQKKP 

       250        260        270        280        290        300 
WSVFRKMETK GLSFEQLSDI FGFRVMVDTV QDCYRALGLI HTTWSMVPGR FKDYISTPKQ 

       310        320        330        340        350        360 
NDYRSIHTTI IGPSRQRIEL QIRTREMDEI AEFGVAAHSI YKDRGSANNP HKISTETNAY 

       370        380        390        400        410        420 
AWLRQTIEQL SEGDNPEEFL EHTKLELFQD QVFCFTPKGR LIALPRGATP IDFAYAVHTD 

       430        440        450        460        470        480 
IGDSCVGAKV NGRIMPLMTE LKNGDEVDII RSKAQVPPAA WESLVATGKA RAAIRRATRS 

       490        500        510        520        530        540 
AVRKQYSGLG MRILERAFER AGKPFSKDIL KPGLPRLARK DVEDVLAAVG RGELPSADVV 

       550        560        570        580        590        600 
KAVYPDYQDT RVTTQNNPAK AGEKGWFNIQ NAAGMIFKVP EGGEGAAAKV DPAATTPKPG 

       610        620        630        640        650        660 
KRALPIRGTN PDLPVRFAPE GAVPGDRIVG ILQPGAGITI YPIQSPALTA YDDQPERWID 

       670        680        690        700        710        720 
VRWDIDDQMS ERFPARISVS AINSPGSLAE IAQIAAANDA NIHNLSMART APDFTEMIID 

       730        740        750 
VEVWDLKHLN RIISQLKESA SVSSAKRVNG 

« Hide

References

« Hide 'large scale' references
[1]"Completion of the genome sequence of Brucella abortus and comparison to the highly similar genomes of Brucella melitensis and Brucella suis."
Halling S.M., Peterson-Burch B.D., Bricker B.J., Zuerner R.L., Qing Z., Li L.-L., Kapur V., Alt D.P., Olsen S.C.
J. Bacteriol. 187:2715-2726(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 9-941.
[2]"Roles of Brucella abortus SpoT in morphological differentiation and intramacrophagic replication."
Kim S., Watanabe K., Suzuki H., Watarai M.
Microbiology 151:1607-1617(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION IN ADAPTATION TO INTRACELLULAR REPLICATION, DISRUPTION PHENOTYPE.
Strain: 544 / Biovar 1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE017223 Genomic DNA. Translation: AAX74044.1.
RefSeqYP_221405.1. NC_006932.1.

3D structure databases

ProteinModelPortalQ57E90.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING262698.BruAb1_0669.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAX74044; AAX74044; BruAb1_0669.
GeneID3339865.
KEGGbmb:BruAb1_0669.
PATRIC17822939. VBIBruAbo15061_0705.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0317.
HOGENOMHOG000018299.
KOK01139.
OMAESNAYSW.
OrthoDBEOG6SV551.
ProtClustDBCLSK2754108.

Enzyme and pathway databases

BioCycBABO262698:GJC2-676-MONOMER.

Family and domain databases

Gene3D3.10.20.30. 1 hit.
InterProIPR026020. (p)ppGpp_Synthase.
IPR002912. ACT_dom.
IPR012675. Beta-grasp_dom.
IPR003607. HD/PDEase_dom.
IPR004811. RelA/Spo_fam.
IPR007685. RelA_SpoT.
IPR004095. TGS.
IPR012676. TGS-like.
[Graphical view]
PANTHERPTHR21262:SF1. PTHR21262:SF1. 1 hit.
PfamPF04607. RelA_SpoT. 1 hit.
PF02824. TGS. 1 hit.
[Graphical view]
SMARTSM00471. HDc. 1 hit.
SM00954. RelA_SpoT. 1 hit.
[Graphical view]
SUPFAMSSF81271. SSF81271. 1 hit.
TIGRFAMsTIGR00691. spoT_relA. 1 hit.
PROSITEPS51671. ACT. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRSH_BRUAB
AccessionPrimary (citable) accession number: Q57E90
Entry history
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: May 10, 2005
Last modified: February 19, 2014
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Brucella abortus strain 9-941

Brucella abortus (strain 9-941): entries and gene names