Reviewed, UniProtKB/Swiss-Prot Q57E90 (RSH_BRUAB)

Names and origin

Protein names

Recommended name:
    GTP pyrophosphokinase rsh
    EC=2.7.6.5
Alternative name(s):
    ATP:GTP 3'-pyrophosphotransferase
    (p)ppGpp synthetase

Gene names

Name:	rsh
Synonyms:	spoT
Ordered Locus Names:	BruAb1_0669

Organism

Brucella abortus [Complete proteome] [HAMAP]

Taxonomic identifier

235 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	750 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Functions as a (p)ppGpp synthetase. In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. Plays a role in adaptation of Brucella to its intracellular host environment. Ref.2
Catalytic activity	ATP + GTP = AMP + guanosine 3'-diphosphate 5'-triphosphate.
Disruption phenotype	Cells show morphological abnormalities such as branching and swelling forms. It does not grow in RPMI-1640 with or without 10% FBS. It shows decreased growth until 48 hours in RPMI-1640 and stationary growth until 48 hours in RPMI-1640 with 10% FBS. It has the same ability as the wild-type to internalize at 0 minute but a lower rate of internalization at 30 minutes. It shows a lower rate of intracellular replication within macrophages than the wild-type. Its adherence is four times higher than that of the wild-type. It shows lower MICs than the wild-type for several antimicrobial agents such as penicillin G, methicillin, erythromycin and doxycycline. At ten days post infection the number of viable bacteria (disruption mutant) is markedly reduced compared to the wild-type. Ref.2
Sequence similarities	Belongs to the relA/spoT family. Contains 1 ACT domain. Contains 1 HD domain.

Ontologies

Keywords
Ligand	ATP-binding GTP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	guanosine tetraphosphate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTP diphosphokinase activity Inferred from electronic annotation. Source: EC amino acid binding Inferred from electronic annotation. Source: InterPro kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 750	750	GTP pyrophosphokinase rsh		PRO_0000322563
Regions
	Domain	45 – 144	100	HD
	Domain	675 – 738	64	ACT

Sequences

	Sequence		Length	Mass (Da)
	Q57E90-1 [UniParc]. Last modified May 10, 2005. Version 1. Checksum: 4836EEECB0E52495	FASTA	750	83,856
	10 20 30 40 50 60 MMRQYELVER VQRYKPDVNE ALLNKAYVYA MQKHGSQKRA SGDPYFSHPL EVAAILTDMH 70 80 90 100 110 120 LDEATIAIAL LHDTIEDTTA TRQEIDQLFG PEIGKLVEGL TKLKKLDLVS KKAVQAENLR 130 140 150 160 170 180 KLLLAISEDV RVLLVKLADR LHNMRTLGVM REDKRLRIAE ETMDIYAPLA GRMGMQDMRE 190 200 210 220 230 240 ELEELAFRYI NPDAWRAVTD RLAELLEKNR GLLQKIETDL SEIFEKNGIK ASVKSRQKKP 250 260 270 280 290 300 WSVFRKMETK GLSFEQLSDI FGFRVMVDTV QDCYRALGLI HTTWSMVPGR FKDYISTPKQ 310 320 330 340 350 360 NDYRSIHTTI IGPSRQRIEL QIRTREMDEI AEFGVAAHSI YKDRGSANNP HKISTETNAY 370 380 390 400 410 420 AWLRQTIEQL SEGDNPEEFL EHTKLELFQD QVFCFTPKGR LIALPRGATP IDFAYAVHTD 430 440 450 460 470 480 IGDSCVGAKV NGRIMPLMTE LKNGDEVDII RSKAQVPPAA WESLVATGKA RAAIRRATRS 490 500 510 520 530 540 AVRKQYSGLG MRILERAFER AGKPFSKDIL KPGLPRLARK DVEDVLAAVG RGELPSADVV 550 560 570 580 590 600 KAVYPDYQDT RVTTQNNPAK AGEKGWFNIQ NAAGMIFKVP EGGEGAAAKV DPAATTPKPG 610 620 630 640 650 660 KRALPIRGTN PDLPVRFAPE GAVPGDRIVG ILQPGAGITI YPIQSPALTA YDDQPERWID 670 680 690 700 710 720 VRWDIDDQMS ERFPARISVS AINSPGSLAE IAQIAAANDA NIHNLSMART APDFTEMIID 730 740 750 VEVWDLKHLN RIISQLKESA SVSSAKRVNG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Completion of the genome sequence of Brucella abortus and comparison to the highly similar genomes of Brucella melitensis and Brucella suis." Halling S.M., Peterson-Burch B.D., Bricker B.J., Zuerner R.L., Qing Z., Li L.-L., Kapur V., Alt D.P., Olsen S.C. J. Bacteriol. 187:2715-2726(2005) [PubMed: 15805518] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 9-941 / Biovar 1.
[2]	"Roles of Brucella abortus SpoT in morphological differentiation and intramacrophagic replication." Kim S., Watanabe K., Suzuki H., Watarai M. Microbiology 151:1607-1617(2005) [PubMed: 15870469] [Abstract] Cited for: FUNCTION IN ADAPTATION TO INTRACELLULAR REPLICATION, DISRUPTION PHENOTYPE. Strain: 544 / Biovar 1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE017223 Genomic DNA. Translation: AAX74044.1.
RefSeq	YP_221405.1.
3D structure databases
HSSP	HSSP built from PDB template 1VJ7 based on UniProtKB Q54089.
ModBase	Search...
Genome annotation databases
GeneID	3339865.
GenomeReviews	Gene locus BruAb1_0669 in contig AE017223_GR.
KEGG	bmb:BruAb1_0669.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG594146.
OMA	INICNIK.
Enzyme and pathway databases
BioCyc	BABO262698:BRUAB1_0669-MON.
BRENDA	2.7.6.5. 575.
Family and domain databases
InterPro	IPR002912. ACT_bd. IPR012675. b-grasp_ferredoxin-like. IPR003607. Met-dep_phosphohydro_HD. IPR006674. Met-dep_phosphohydro_HD_sub. IPR007685. RelA_SpoT. IPR004811. SpoT_relA. IPR004095. TGS. IPR012676. TGS-like. [Graphical view]
Gene3D	G3DSA:3.10.20.30. Ferredoxin_fold. 1 hit.
Pfam	PF01842. ACT. 1 hit. PF01966. HD. 1 hit. PF04607. RelA_SpoT. 1 hit. PF02824. TGS. 1 hit. [Graphical view]
SMART	SM00471. HDc. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RSH_BRUAB

Accession

Primary (citable) accession number: Q57E90

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	May 10, 2005
Last modified:	December 15, 2009
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)