Q57E90 (RSH_BRUAB) Reviewed, UniProtKB/Swiss-Prot
Last modified May 29, 2013. Version 64. History...
Names and origin
|Protein names||Recommended name:|
GTP pyrophosphokinase rsh
|Organism||Brucella abortus biovar 1 (strain 9-941) [Complete proteome] [HAMAP]|
|Taxonomic identifier||262698 [NCBI]|
|Taxonomic lineage||Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella ›|
|Sequence length||750 AA.|
|Protein existence||Evidence at protein level|
General annotation (Comments)
Functions as a (p)ppGpp synthase. In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. Plays a role in adaptation of Brucella to its intracellular host environment. Ref.2
ATP + GTP = AMP + guanosine 3'-diphosphate 5'-triphosphate.
Cells show morphological abnormalities such as branching and swelling forms. It does not grow in RPMI-1640 with or without 10% FBS. It shows decreased growth until 48 hours in RPMI-1640 and stationary growth until 48 hours in RPMI-1640 with 10% FBS. It has the same ability as the wild-type to internalize at 0 minute but a lower rate of internalization at 30 minutes. It shows a lower rate of intracellular replication within macrophages than the wild-type. Its adherence is four times higher than that of the wild-type. It shows lower MICs than the wild-type for several antimicrobial agents such as penicillin G, methicillin, erythromycin and doxycycline. At ten days post infection the number of viable bacteria (disruption mutant) is markedly reduced compared to the wild-type. Ref.2
Belongs to the RelA/SpoT family.
Contains 1 ACT domain.
Contains 1 HD domain.
|Technical term||Complete proteome|
|Gene Ontology (GO)|
|Biological_process||guanosine tetraphosphate metabolic process|
Inferred from electronic annotation. Source: InterPro
Inferred from electronic annotation. Source: UniProtKB-KWGTP binding
Inferred from electronic annotation. Source: UniProtKB-KWGTP diphosphokinase activity
Inferred from electronic annotation. Source: ECkinase activity
Inferred from electronic annotation. Source: UniProtKB-KW
|Complete GO annotation...|
Sequence annotation (Features)
|||"Completion of the genome sequence of Brucella abortus and comparison to the highly similar genomes of Brucella melitensis and Brucella suis."|
Halling S.M., Peterson-Burch B.D., Bricker B.J., Zuerner R.L., Qing Z., Li L.-L., Kapur V., Alt D.P., Olsen S.C.
J. Bacteriol. 187:2715-2726(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
|||"Roles of Brucella abortus SpoT in morphological differentiation and intramacrophagic replication."|
Kim S., Watanabe K., Suzuki H., Watarai M.
Microbiology 151:1607-1617(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION IN ADAPTATION TO INTRACELLULAR REPLICATION, DISRUPTION PHENOTYPE.
Strain: 544 / Biovar 1.
|AE017223 Genomic DNA. Translation: AAX74044.1.|
|RefSeq||YP_221405.1. NC_006932.1. |
3D structure databases
Protein-protein interaction databases
Protocols and materials databases
Genome annotation databases
|EnsemblBacteria||AAX74044; AAX74044; BruAb1_0669. |
|PATRIC||17822939. VBIBruAbo15061_0705. |
Enzyme and pathway databases
Family and domain databases
|Gene3D||18.104.22.168. 1 hit. |
|InterPro||IPR026020. (p)ppGpp_Synthase. |
|PANTHER||PTHR21262. PTHR21262. 1 hit. |
|Pfam||PF04607. RelA_SpoT. 1 hit. |
PF02824. TGS. 1 hit.
|SMART||SM00471. HDc. 1 hit. |
SM00954. RelA_SpoT. 1 hit.
|SUPFAM||SSF81271. TGS-like. 1 hit. |
|TIGRFAMs||TIGR00691. spoT_relA. 1 hit. |
|Accession||Primary (citable) accession number: Q57E90|
|Entry status||Reviewed (UniProtKB/Swiss-Prot)|
|Annotation program||Prokaryotic Protein Annotation Program|