Reviewed, UniProtKB/Swiss-Prot Q57E89 (PYRE_BRUAB)

Names and origin

Protein names

Recommended name:
    Orotate phosphoribosyltransferase
      Short name=OPRT
      Short name=OPRTase
    EC=2.4.2.10

Gene names

Name:	pyrE
Ordered Locus Names:	BruAb1_0670

Organism

Brucella abortus [Complete proteome] [HAMAP]

Taxonomic identifier

235 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	192 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP) By similarity. HAMAP MF_01208
Catalytic activity	Orotidine 5'-phosphate + diphosphate = orotate + 5-phospho-alpha-D-ribose 1-diphosphate. HAMAP MF_01208
Cofactor	Magnesium By similarity. HAMAP MF_01208
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 1/2. HAMAP MF_01208
Subunit structure	Homodimer By similarity. HAMAP MF_01208
Sequence similarities	Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Ligand	Magnesium
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	nucleoside metabolic process Inferred from electronic annotation. Source: InterPro pyrimidine base biosynthetic process Inferred from electronic annotation. Source: InterPro pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	magnesium ion binding Inferred from electronic annotation. Source: HAMAP orotate phosphoribosyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 192	192	Orotate phosphoribosyltransferase HAMAP MF_01208		PRO_1000066214
Regions
	Region	116 – 124	9	5-phosphoribose 1-diphosphate binding By similarity
Sites
	Binding site	120	1	Orotate By similarity
	Binding site	148	1	Orotate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q57E89-1 [UniParc]. Last modified May 10, 2005. Version 1. Checksum: 17CA2F3F7CEF83C7	FASTA	192	20,733
	10 20 30 40 50 60 MNTDDVLAVF REAGAILEGH FILTSGLRSP VFLQKARVFM HADKTEKLCK ALAEKIRAAD 70 80 90 100 110 120 LGPIDYVVGP AIGGLIPSYE TSRHLGVPSV WVERENGVFR LRRFDVPKGA RVVIVEDIVT 130 140 150 160 170 180 TGLSIRETID CMKDLGIEVV AAACIVDRSA GKADVGTRLI SLAEYEVPAY PADKLPPELA 190 AIPAVKPGSR NI

References

[1]

"Completion of the genome sequence of Brucella abortus and comparison to the highly similar genomes of Brucella melitensis and Brucella suis." Halling S.M., Peterson-Burch B.D., Bricker B.J., Zuerner R.L., Qing Z., Li L.-L., Kapur V., Alt D.P., Olsen S.C. J. Bacteriol. 187:2715-2726(2005) [PubMed: 15805518] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 9-941 / Biovar 1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017223 Genomic DNA. Translation: AAX74045.1.
RefSeq	YP_221406.1.
3D structure databases
SMR	Q57E89. Positions 4-173.
ModBase	Search...
Genome annotation databases
GeneID	3340784.
GenomeReviews	Gene locus BruAb1_0670 in contig AE017223_GR.
KEGG	bmb:BruAb1_0670.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG404341.
OMA	FYLQSAK.
Enzyme and pathway databases
BioCyc	BABO262698:BRUAB1_0670-MONOMER.
BRENDA	2.4.2.10. 575.
Family and domain databases
HAMAP	MF_01208_B. PyrE_B. [Tree]
InterPro	IPR006273. Orotate_PRibTrfase_thermus-typ. IPR000836. PRibTrfase. [Graphical view]
Pfam	PF00156. Pribosyltran. 1 hit. [Graphical view]
TIGRFAMs	TIGR01367. pyrE_Therm. 1 hit.
PROSITE	PS00103. PUR_PYR_PR_TRANSFER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRE_BRUAB

Accession

Primary (citable) accession number: Q57E89

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	May 10, 2005
Last modified:	February 9, 2010
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)