Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

6,7-dimethyl-8-ribityllumazine synthase 1

Gene

ribH1

Organism
Brucella abortus biovar 1 (strain 9-941)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of 6,7-dimethyl-8-ribityllumazine by condensation of 5-amino-6-(D-ribitylamino)uracil with 3,4-dihydroxy-2-butanone 4-phosphate. This is the penultimate step in the biosynthesis of riboflavin.UniRule annotation

Catalytic activityi

1-deoxy-L-glycero-tetrulose 4-phosphate + 5-amino-6-(D-ribitylamino)uracil = 6,7-dimethyl-8-(D-ribityl)lumazine + 2 H2O + phosphate.UniRule annotation

Pathwayi: riboflavin biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes riboflavin from 2-hydroxy-3-oxobutyl phosphate and 5-amino-6-(D-ribitylamino)uracil.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. 6,7-dimethyl-8-ribityllumazine synthase 1 (ribH1), 6,7-dimethyl-8-ribityllumazine synthase 2 (ribH2)
  2. no protein annotated in this organism
This subpathway is part of the pathway riboflavin biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes riboflavin from 2-hydroxy-3-oxobutyl phosphate and 5-amino-6-(D-ribitylamino)uracil, the pathway riboflavin biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei22 – 2215-amino-6-(D-ribitylamino)uracilUniRule annotation
Active sitei90 – 901Proton donorUniRule annotation
Binding sitei115 – 11515-amino-6-(D-ribitylamino)uracil; via amide nitrogen and carbonyl oxygenUniRule annotation
Binding sitei129 – 12911-deoxy-L-glycero-tetrulose 4-phosphateUniRule annotation
Binding sitei137 – 13715-amino-6-(D-ribitylamino)uracilUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Riboflavin biosynthesis

Enzyme and pathway databases

BioCyciBABO262698:GJC2-794-MONOMER.
BRENDAi2.5.1.78. 994.
UniPathwayiUPA00275; UER00404.

Names & Taxonomyi

Protein namesi
Recommended name:
6,7-dimethyl-8-ribityllumazine synthase 1UniRule annotation (EC:2.5.1.78UniRule annotation)
Short name:
DMRL synthase 1UniRule annotation
Short name:
LS 1UniRule annotation
Short name:
Lumazine synthase 1UniRule annotation
Alternative name(s):
Type I lumazine synthaseBy similarity
Gene namesi
Name:ribH1UniRule annotation
Synonyms:ribH, ribH-1
Ordered Locus Names:BruAb1_0785
OrganismiBrucella abortus biovar 1 (strain 9-941)
Taxonomic identifieri262698 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
Proteomesi
  • UP000000540 Componenti: Chromosome I

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 1571576,7-dimethyl-8-ribityllumazine synthase 1PRO_0000134724Add
BLAST

Expressioni

Inductioni

The two ribH genes may be differentially expressed during the Brucella infection cycle. Brucella would use RibH1 for flavin biosynthesis during the extracellular phase and RibH2 during intracellular growth.By similarity

Interactioni

Subunit structurei

Homopentamer.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ57DY1.
SMRiQ57DY1. Positions 11-157.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ57DY1.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni53 – 5535-amino-6-(D-ribitylamino)uracil bindingUniRule annotation
Regioni82 – 8435-amino-6-(D-ribitylamino)uracil bindingUniRule annotation
Regioni87 – 8821-deoxy-L-glycero-tetrulose 4-phosphate bindingUniRule annotation

Sequence similaritiesi

Belongs to the DMRL synthase family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000229250.
KOiK00794.
OMAiTVCNDSS.

Family and domain databases

Gene3Di3.40.50.960. 1 hit.
HAMAPiMF_00178. Lumazine_synth. 1 hit.
InterProiIPR002180. DMRL_synthase.
[Graphical view]
PANTHERiPTHR21058. PTHR21058. 1 hit.
PfamiPF00885. DMRL_synthase. 1 hit.
[Graphical view]
SUPFAMiSSF52121. SSF52121. 1 hit.
TIGRFAMsiTIGR00114. lumazine-synth. 1 hit.

Sequencei

Sequence statusi: Complete.

Q57DY1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEFLMSKHEA DAPHLLIVEA RFYDDLADAL LDGAKAALDE AGATYDVVTV
60 70 80 90 100
PGALEIPATI SFALDGADNG GTEYDGFVAL GTVIRGETYH FDIVSNESCR
110 120 130 140 150
ALTDLSVEES IAIGNGILTV ENEEQAWVHA RREDKDKGGF AARAALTMIG

LRKKFGA
Length:157
Mass (Da):16,791
Last modified:May 10, 2005 - v1
Checksum:i657DE105F885A875
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017223 Genomic DNA. Translation: AAX74153.1.

Genome annotation databases

EnsemblBacteriaiAAX74153; AAX74153; BruAb1_0785.
KEGGibmb:BruAb1_0785.
PATRICi17823186. VBIBruAbo15061_0827.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017223 Genomic DNA. Translation: AAX74153.1.

3D structure databases

ProteinModelPortaliQ57DY1.
SMRiQ57DY1. Positions 11-157.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAX74153; AAX74153; BruAb1_0785.
KEGGibmb:BruAb1_0785.
PATRICi17823186. VBIBruAbo15061_0827.

Phylogenomic databases

HOGENOMiHOG000229250.
KOiK00794.
OMAiTVCNDSS.

Enzyme and pathway databases

UniPathwayiUPA00275; UER00404.
BioCyciBABO262698:GJC2-794-MONOMER.
BRENDAi2.5.1.78. 994.

Miscellaneous databases

EvolutionaryTraceiQ57DY1.

Family and domain databases

Gene3Di3.40.50.960. 1 hit.
HAMAPiMF_00178. Lumazine_synth. 1 hit.
InterProiIPR002180. DMRL_synthase.
[Graphical view]
PANTHERiPTHR21058. PTHR21058. 1 hit.
PfamiPF00885. DMRL_synthase. 1 hit.
[Graphical view]
SUPFAMiSSF52121. SSF52121. 1 hit.
TIGRFAMsiTIGR00114. lumazine-synth. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRISB1_BRUAB
AccessioniPrimary (citable) accession number: Q57DY1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 21, 2005
Last sequence update: May 10, 2005
Last modified: September 7, 2016
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Brucella abortus strain 9-941
    Brucella abortus (strain 9-941): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.