Q57DK9 (TIG_BRUAB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Trigger factor
Short name=TF
EC=5.2.1.8

Alternative name(s):
PPIase

Gene names

Name:	tig
Ordered Locus Names:	BruAb1_0910

Organism

Brucella abortus biovar 1 (strain 9-941) [Complete proteome] [HAMAP]

Taxonomic identifier

262698 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella › Brucella abortus › Brucella abortus bv. 1

Protein attributes

Sequence length	471 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation. Functions as a peptidyl-prolyl cis-trans isomerase By similarity. HAMAP-Rule MF_00303
Catalytic activity	Peptidylproline (omega=180) = peptidylproline (omega=0). HAMAP-Rule MF_00303
Subcellular location	Cytoplasm. Note: About half TF is bound to the ribosome near the polypeptide exit tunnel while the other half is free in the cytoplasm By similarity. HAMAP-Rule MF_00303
Domain	Consists of 3 domains; the N-terminus binds the ribosome, the middle domain has PPIase activity, while the C-terminus has intrinsic chaperone activity on its own By similarity. HAMAP-Rule MF_00303
Sequence similarities	Belongs to the FKBP-type PPIase family. Tig subfamily. Contains 1 PPIase FKBP-type domain.
Sequence caution	The sequence AAX74275.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Cell cycle Cell division
Cellular component	Cytoplasm
Molecular function	Chaperone Isomerase Rotamase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW protein folding Inferred from electronic annotation. Source: HAMAP protein peptidyl-prolyl isomerization Inferred from electronic annotation. Source: GOC protein transport Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	peptidyl-prolyl cis-trans isomerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 471	471	Trigger factor HAMAP-Rule MF_00303		PRO_0000256532
Regions
	Domain	169 – 254	86	PPIase FKBP-type

Sequences

	Sequence		Length	Mass (Da)
	Q57DK9 [UniParc]. Last modified October 31, 2006. Version 2. Checksum: AED56918C5642E41	FASTA	471	52,937
	10 20 30 40 50 60 MQVTETLNEG LKREIKVVVP AGDLEAKLAE RLETARGRAR INGFRPGKVP TAHLRKMYGK 70 80 90 100 110 120 SFMAEIVNEI LNDSSRSILA ERNEKSATQP EVIMSEDEKE AEKVLDGKAD FVFSLNYEVL 130 140 150 160 170 180 PAIEVKDFSK IAVTREVVDI SDEEVDEQVK RIASSTRTFE TKKGKAENED RVTIDYLGKL 190 200 210 220 230 240 DGEPFEGGAD NDAQLVLGSG QFIPGFEEQL IGLKAGDEKV ITVTFPAEYG AAHLAGKEAT 250 260 270 280 290 300 FDIKVKEVAK PNELVLDDET AKKLGIESLE RLRQVVREQI ESQYGQITRQ KVKRQILDAL 310 320 330 340 350 360 DGDYQFETPQ KLVDAEFNNI WQQINFDLQQ AGRTFEDEET TEEAAREEYR KLAERRVRLG 370 380 390 400 410 420 LVLSEIGEKA GVEVTEEELQ RAVYDQVRRY PGQEKEIYDF LRRTPDAVAN LRAPIFEEKV 430 440 450 460 470 VDHLLANINV TDKKVSKEEL TAEDEDAASE AKPAKKAAAK KKAEEGKSEE A

References

[1]

"Completion of the genome sequence of Brucella abortus and comparison to the highly similar genomes of Brucella melitensis and Brucella suis." Halling S.M., Peterson-Burch B.D., Bricker B.J., Zuerner R.L., Qing Z., Li L.-L., Kapur V., Alt D.P., Olsen S.C. J. Bacteriol. 187:2715-2726(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 9-941.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017223 Genomic DNA. Translation: AAX74275.1. Different initiation.
RefSeq	YP_221636.1. NC_006932.1.
3D structure databases
HSSP	HSSP built from PDB template 1L1P based on UniProtKB P0A850.
ProteinModelPortal	Q57DK9.
ModBase	Search...
Protein-protein interaction databases
STRING	262698.BruAb1_0910.
Proteomic databases
PRIDE	Q57DK9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAX74275; AAX74275; BruAb1_0910.
GeneID	3340906.
KEGG	bmb:BruAb1_0910.
PATRIC	17823454. VBIBruAbo15061_0957.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0544.
HOGENOM	HOG000218241.
KO	K03545.
OMA	FPADYHG.
ProtClustDB	PRK01490.
Enzyme and pathway databases
BioCyc	BABO262698:GJC2-923-MONOMER.
Family and domain databases
Gene3D	1.10.3120.10. 1 hit. 3.30.70.1050. 1 hit.
HAMAP	MF_00303. Trigger_factor_Tig.
InterPro	IPR001179. PPIase_FKBP_dom. IPR005215. Trig_fac. IPR008880. Trigger_fac_C. IPR008881. Trigger_fac_ribosome-bd_bac. IPR027304. Trigger_fact/SurA_dom. [Graphical view]
Pfam	PF00254. FKBP_C. 1 hit. PF05698. Trigger_C. 1 hit. PF05697. Trigger_N. 1 hit. [Graphical view]
PIRSF	PIRSF003095. Trigger_factor. 1 hit.
SUPFAM	SSF109998. Trigger_fac_C_bac. 1 hit. SSF102735. Trigger_fac_ribosome-bd_bac. 1 hit.
TIGRFAMs	TIGR00115. tig. 1 hit.
PROSITE	PS50059. FKBP_PPIASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TIG_BRUAB

Accession

Primary (citable) accession number: Q57DK9

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2006
Last sequence update:	October 31, 2006
Last modified:	May 1, 2013
This is version 54 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program