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Q57DI5 (SYY_BRUAB) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 54. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Tyrosine--tRNA ligase

EC=6.1.1.1
Alternative name(s):
Tyrosyl-tRNA synthetase
Short name=TyrRS
Gene names
Name:tyrS
Ordered Locus Names:BruAb1_0935
OrganismBrucella abortus biovar 1 (strain 9-941) [Complete proteome] [HAMAP]
Taxonomic identifier262698 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella

Protein attributes

Sequence length417 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr) By similarity. HAMAP-Rule MF_02006

Catalytic activity

ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + L-tyrosyl-tRNA(Tyr). HAMAP-Rule MF_02006

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_02006

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_02006.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family. TyrS type 1 subfamily.

Contains 1 S4 RNA-binding domain.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
RNA-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processtyrosyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

RNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

tyrosine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 417417Tyrosine--tRNA ligase HAMAP-Rule MF_02006
PRO_0000234688

Regions

Domain350 – 41768S4 RNA-binding
Motif44 – 5310"HIGH" region HAMAP-Rule MF_02006
Motif236 – 2405"KMSKS" region HAMAP-Rule MF_02006

Sites

Binding site391Tyrosine By similarity
Binding site1761Tyrosine By similarity
Binding site1801Tyrosine By similarity
Binding site2391ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q57DI5 [UniParc].

Last modified May 10, 2005. Version 1.
Checksum: 2D734F3E7A2D83EC

FASTA41746,157
        10         20         30         40         50         60 
MSGFKSDFLR TLSERGFIHQ ISDESGLDEL LAKETVTAYI GFDPTAPSLH AGGLIQIMML 

        70         80         90        100        110        120 
YWLQQTGHKP VALMGGGTGM VGDPSFKDEA RKLMTEDTIA ENMASIKRVF ANYLTFGDGA 

       130        140        150        160        170        180 
NDALMVNNGE WLRNINYLEF LRDVGRHFSV NRMLSFDSVK LRLDREQSLS FLEFNYMILQ 

       190        200        210        220        230        240 
AYDFVELNKR YGLRLQMGGS DQWGNIVNGI DLGHRMGTPQ LYALTSPLLT TASGQKMGKS 

       250        260        270        280        290        300 
LGGAIWLNAD MLSAYDFWQY WRNTEDADVE RFLKLYTTLP LDEIARLAEL GGAEINEAKK 

       310        320        330        340        350        360 
ILATEVTAML HGRDAAEEAA ETARKTFEDG ELSENLPTVG VHKATLNDGI GVLALMVLAE 

       370        380        390        400        410 
LCTTNGEARR HVEGGAVRIN DEPVSDPRMV VNAAALNDQG LIKLSLGKKR HVLIRPA 

« Hide

References

[1]"Completion of the genome sequence of Brucella abortus and comparison to the highly similar genomes of Brucella melitensis and Brucella suis."
Halling S.M., Peterson-Burch B.D., Bricker B.J., Zuerner R.L., Qing Z., Li L.-L., Kapur V., Alt D.P., Olsen S.C.
J. Bacteriol. 187:2715-2726(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 9-941.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE017223 Genomic DNA. Translation: AAX74299.1.
RefSeqYP_221660.1. NC_006932.1.

3D structure databases

ProteinModelPortalQ57DI5.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING262698.BruAb1_0935.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAX74299; AAX74299; BruAb1_0935.
GeneID3341098.
KEGGbmb:BruAb1_0935.
PATRIC17823508. VBIBruAbo15061_0984.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0162.
HOGENOMHOG000242790.
KOK01866.
OMATTIRYNS.
OrthoDBEOG6B09VR.

Enzyme and pathway databases

BioCycBABO262698:GJC2-949-MONOMER.

Family and domain databases

Gene3D3.10.290.10. 1 hit.
3.40.50.620. 1 hit.
HAMAPMF_02006. Tyr_tRNA_synth_type1.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002305. aa-tRNA-synth_Ic.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002942. S4_RNA-bd.
IPR002307. Tyr-tRNA-ligase.
IPR024088. Tyr-tRNA-ligase_bac-type.
IPR024107. Tyr-tRNA-ligase_bac_1.
[Graphical view]
PANTHERPTHR11766. PTHR11766. 1 hit.
PfamPF00579. tRNA-synt_1b. 1 hit.
[Graphical view]
PRINTSPR01040. TRNASYNTHTYR.
TIGRFAMsTIGR00234. tyrS. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
PS50889. S4. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYY_BRUAB
AccessionPrimary (citable) accession number: Q57DI5
Entry history
Integrated into UniProtKB/Swiss-Prot: May 16, 2006
Last sequence update: May 10, 2005
Last modified: May 14, 2014
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Brucella abortus strain 9-941

Brucella abortus (strain 9-941): entries and gene names

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries