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Protein

GTP 3',8-cyclase

Gene

moaA

Organism
Brucella abortus biovar 1 (strain 9-941)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the cyclization of GTP to (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate.UniRule annotation

Catalytic activityi

GTP + S-adenosyl-L-methionine + reduced electron acceptor = (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate + 5'-deoxyadenosine + L-methionine + oxidized electron acceptor.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 2 [4Fe-4S] clusters. Binds 1 [4Fe-4S] cluster coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine and 1 [4Fe-4S] cluster coordinated with 3 cysteines and the GTP-derived substrate.UniRule annotation

Pathwayi: molybdopterin biosynthesis

This protein is involved in the pathway molybdopterin biosynthesis, which is part of Cofactor biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway molybdopterin biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei28GTPUniRule annotation1
Metal bindingi35Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi39Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation1
Binding sitei41S-adenosyl-L-methionineUniRule annotation1
Metal bindingi42Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation1
Binding sitei77GTPUniRule annotation1
Binding sitei81S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation1
Binding sitei111GTPUniRule annotation1
Binding sitei135S-adenosyl-L-methionineUniRule annotation1
Binding sitei171GTPUniRule annotation1
Binding sitei205S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygenUniRule annotation1
Metal bindingi268Iron-sulfur 2 (4Fe-4S-substrate)UniRule annotation1
Metal bindingi271Iron-sulfur 2 (4Fe-4S-substrate)UniRule annotation1
Metal bindingi285Iron-sulfur 2 (4Fe-4S-substrate)UniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi273 – 275GTPUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Molybdenum cofactor biosynthesis

Keywords - Ligandi

4Fe-4S, GTP-binding, Iron, Iron-sulfur, Metal-binding, Nucleotide-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00344.

Names & Taxonomyi

Protein namesi
Recommended name:
GTP 3',8-cyclaseUniRule annotation (EC:4.1.99.22UniRule annotation)
Alternative name(s):
Molybdenum cofactor biosynthesis protein AUniRule annotation
Gene namesi
Name:moaAUniRule annotation
Ordered Locus Names:BruAb1_0964
OrganismiBrucella abortus biovar 1 (strain 9-941)
Taxonomic identifieri262698 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
Proteomesi
  • UP000000540 Componenti: Chromosome I

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000541761 – 344GTP 3',8-cyclaseAdd BLAST344

Interactioni

Subunit structurei

Monomer and homodimer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ57DG3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. MoaA family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000228680.
KOiK03639.
OMAiGWIHQIR.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01225_B. MoaA_B. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR013483. MoaA.
IPR000385. MoaA_NifB_PqqE_Fe-S-bd_CS.
IPR010505. Mob_synth_C.
IPR007197. rSAM.
[Graphical view]
PfamiPF06463. Mob_synth_C. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02666. moaA. 1 hit.
PROSITEiPS01305. MOAA_NIFB_PQQE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q57DG3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRNVQDQPLV SPTEPMIDPF GRAVTYLRVS VTDRCDFRCT YCMAEHMTFL
60 70 80 90 100
PKKDLLTLEE LDRLCSVFIE KGVRKLRLTG GEPLVRKNIM HLIGNLSRHL
110 120 130 140 150
KSGALDELTL TTNGSQLARF AGELADCGVR RINVSLDTLN PEKFRTITRW
160 170 180 190 200
GDLSRVLEGI DAAQKAAIHV KINAVALKDF NDAEIPELIR WAHGRGMDVT
210 220 230 240 250
LIETMPMGEI EFDRTDQYLP LSQVRADLAS QFTLADIPYR TGGPARYVTI
260 270 280 290 300
SETGGRLGFI TPMTYNFCES CNRVRLTCTG MLYMCLGQND DADLRKALRE
310 320 330 340
SESDEHLSQA IDEAISRKPK GHDFIIDREH NRPSVARHMS LTGG
Length:344
Mass (Da):38,687
Last modified:May 10, 2005 - v1
Checksum:i50B8258570E51148
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017223 Genomic DNA. Translation: AAX74321.1.
RefSeqiWP_002966802.1. NC_006932.1.

Genome annotation databases

EnsemblBacteriaiAAX74321; AAX74321; BruAb1_0964.
GeneIDi3787635.
KEGGibmb:BruAb1_0964.
PATRICi17823572. VBIBruAbo15061_1015.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017223 Genomic DNA. Translation: AAX74321.1.
RefSeqiWP_002966802.1. NC_006932.1.

3D structure databases

ProteinModelPortaliQ57DG3.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAX74321; AAX74321; BruAb1_0964.
GeneIDi3787635.
KEGGibmb:BruAb1_0964.
PATRICi17823572. VBIBruAbo15061_1015.

Phylogenomic databases

HOGENOMiHOG000228680.
KOiK03639.
OMAiGWIHQIR.

Enzyme and pathway databases

UniPathwayiUPA00344.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01225_B. MoaA_B. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR013483. MoaA.
IPR000385. MoaA_NifB_PqqE_Fe-S-bd_CS.
IPR010505. Mob_synth_C.
IPR007197. rSAM.
[Graphical view]
PfamiPF06463. Mob_synth_C. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02666. moaA. 1 hit.
PROSITEiPS01305. MOAA_NIFB_PQQE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMOAA_BRUAB
AccessioniPrimary (citable) accession number: Q57DG3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: May 10, 2005
Last modified: November 30, 2016
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Brucella abortus strain 9-941
    Brucella abortus (strain 9-941): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.