Reviewed, UniProtKB/Swiss-Prot Q57D18 (ISPDF_BRUAB)

Names and origin

Protein names

Recommended name:
    Bifunctional enzyme ispD/ispF
Including the following 2 domains:
    1- Recommended name:
            2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase
              EC=2.7.7.60
        Alternative name(s):
            4-diphosphocytidyl-2C-methyl-D-erythritol synthase
            MEP cytidylyltransferase
              Short name=MCT
    2- Recommended name:
            2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase
                Short name=MECPS
                Short name=MECDP-synthase
              EC=4.6.1.12

Gene names

Name:	ispDF
Ordered Locus Names:	BruAb1_1126

Organism

Brucella abortus [Complete proteome] [HAMAP]

Taxonomic identifier

235 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	390 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Bifunctional enzyme that catalyzes the formation of 4-diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4-phosphate (MEP) (ispD), and converts 4-diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate into 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MECDP) and CMP (ispF) By similarity.
Catalytic activity	CTP + 2-C-methyl-D-erythritol 4-phosphate = diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_01520 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = 2-C-methyl-D-erythritol 2,4-cyclodiphosphate + CMP. HAMAP MF_01520
Cofactor	Divalent metal cations By similarity.
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 2/6. HAMAP MF_01520 Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 4/6.
Sequence similarities	In the N-terminal section; belongs to the ispD family. In the C-terminal section; belongs to the ispF family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	Metal-binding
Molecular function	Lyase Nucleotidyltransferase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase activity Inferred from electronic annotation. Source: HAMAP 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase activity Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 390	390	Bifunctional enzyme ispD/ispF HAMAP MF_01520		PRO_0000075659
Regions
	Region	1 – 229	229	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase HAMAP MF_01520
	Region	230 – 390	161	2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase HAMAP MF_01520
Sites
	Metal binding	236	1	Divalent metal cation By similarity
	Metal binding	238	1	Divalent metal cation By similarity
	Metal binding	270	1	Divalent metal cation By similarity
	Site	8	1	Transition state stabilizer By similarity
	Site	17	1	Transition state stabilizer By similarity
	Site	148	1	Positions MEP for the nucleophilic attack By similarity
	Site	205	1	Positions MEP for the nucleophilic attack By similarity
	Site	262	1	Transition state stabilizer By similarity
	Site	361	1	Transition state stabilizer By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q57D18-1 [UniParc]. Last modified May 10, 2005. Version 1. Checksum: 05AB0A2FBA03DEDB	FASTA	390	41,840
	10 20 30 40 50 60 MAAGRGERAG QSAEGPKQYR LIGAEAVLAR TLRAFTDCPL IGTIAVVIHP DDHALYRRAV 70 80 90 100 110 120 PEKHENVILV TGGPTRQEST RLGLLALKDE APQYVLIHDG VRPFIGQDLL ERIIANLTPD 130 140 150 160 170 180 NGVLPALAVS DTLKRAAADG MVETTISRTG LFAAQTPQAF PYAPILDAHE KAFAINRTDF 190 200 210 220 230 240 TDDAAIAEWQ EIAVRIIEGS ADNTKLTWAK DIEMADKRLR QDHAVFPDIR TGNGYDVHSF 250 260 270 280 290 300 EPGDHVTLCG VKIPHEAKLN GHSDADVALH ALTDALLATR GAGDIGTHFP PSDPQWKGAA 310 320 330 340 350 360 SRIFIEHAAK IVREAGGRIA NVDVTLISEA PKIGPHRAAM TQALCDMLGI AADRVSIKAT 370 380 390 TNEKLGFVGR REGIAAIATA TVIYPGEVPE

References

[1]

"Completion of the genome sequence of Brucella abortus and comparison to the highly similar genomes of Brucella melitensis and Brucella suis." Halling S.M., Peterson-Burch B.D., Bricker B.J., Zuerner R.L., Qing Z., Li L.-L., Kapur V., Alt D.P., Olsen S.C. J. Bacteriol. 187:2715-2726(2005) [PubMed: 15805518] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 9-941 / Biovar 1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE017223 Genomic DNA. Translation: AAX74466.1.
RefSeq	YP_221827.2.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3340471.
GenomeReviews	Gene locus BruAb1_1126 in contig AE017223_GR.
KEGG	bmb:BruAb1_1126.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q57D18.
OMA	IVLIHDA.
Enzyme and pathway databases
BioCyc	BABO262698:BRUAB1_1126-MON.
BRENDA	2.7.7.60. 575. 4.6.1.12. 575.
Family and domain databases
HAMAP	MF_01520. [Tree]
InterPro	IPR001228. ISPD_synthase. IPR018294. ISPD_synthase_CS. IPR003526. MECDP_synthase_core. IPR020555. MECDP_synthase_CS. [Graphical view]
Gene3D	G3DSA:3.30.1330.50. MECDP_synthase_core. 1 hit.
Pfam	PF01128. IspD. 1 hit. PF02542. YgbB. 1 hit. [Graphical view]
TIGRFAMs	TIGR00453. ispD. 1 hit. TIGR00151. ispF. 1 hit.
PROSITE	PS01295. ISPD. 1 hit. PS01350. ISPF. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ISPDF_BRUAB

Accession

Primary (citable) accession number: Q57D18

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2005
Last sequence update:	May 10, 2005
Last modified:	November 3, 2009
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)