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Q57D05 (PYRG_BRUAB) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 64. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
CTP synthase

EC=6.3.4.2
Alternative name(s):
CTP synthetase
UTP--ammonia ligase
Gene names
Name:pyrG
Ordered Locus Names:BruAb1_1140
OrganismBrucella abortus biovar 1 (strain 9-941) [Complete proteome] [HAMAP]
Taxonomic identifier262698 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella

Protein attributes

Sequence length542 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen By similarity. HAMAP-Rule MF_01227

Catalytic activity

ATP + UTP + L-glutamine = ADP + phosphate + CTP + L-glutamate. HAMAP-Rule MF_01227

Enzyme regulation

Allosterically activated by GTP, when glutamine is the substrate. Inhibited by CTP By similarity. HAMAP-Rule MF_01227

Pathway

Pyrimidine metabolism; CTP biosynthesis via de novo pathway; CTP from UDP: step 2/2. HAMAP-Rule MF_01227

Subunit structure

Homotetramer By similarity. HAMAP-Rule MF_01227

Sequence similarities

Belongs to the CTP synthase family.

Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
   Biological processPyrimidine biosynthesis
   DomainGlutamine amidotransferase
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_process'de novo' CTP biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

glutamine metabolic process

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

CTP synthase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 542542CTP synthase HAMAP-Rule MF_01227
PRO_0000266079

Regions

Domain291 – 541251Glutamine amidotransferase type-1
Region1 – 252252Aminator domain HAMAP-Rule MF_01227

Sites

Active site3801Nucleophile By similarity
Active site5141 By similarity
Active site5161 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q57D05 [UniParc].

Last modified May 10, 2005. Version 1.
Checksum: BBA3FDE07C633A9A

FASTA54260,063
        10         20         30         40         50         60 
MARYVFITGG VVSSLGKGIA AAALAALLQA RGYRVRIRKL DPYLNVDPGT MSPYQHGEVF 

        70         80         90        100        110        120 
VTDDGAETDL DLGHYERFTG RPANQQDNIT TGRIYRNIIE KERRGDYLGA TVQVIPHVTD 

       130        140        150        160        170        180 
EIKNFVLEGN EDYDFVLCEI GGTVGDIEAM PFLEAIRQLG NELPRGTAVY IHLTLMPYIP 

       190        200        210        220        230        240 
AAGELKTKPT QHSVKELRSI GIAPDILLVR ADREIPESDR RKLSLFCNVR ESAVIQALDV 

       250        260        270        280        290        300 
ATIYDVPIAY HKEGLDSEVL SAFGIDPAPK PRMDRWEEVS HRLHNPEGEV TIAVVGKYTG 

       310        320        330        340        350        360 
LKDAYKSLIE ALHHGGLANK VKVNLDWIEA EVFESEDPAP YLEKVHGILV PGGFGERGAE 

       370        380        390        400        410        420 
GKILAAKFAR ERKVPYFGIC FGMQMACIEA ARNLVGIEDA SSSEFGPTRE PVVGLMTEWL 

       430        440        450        460        470        480 
KGNMLEKRAA AGDLGGTMRL GAYEAVLKPD SKIAQIYGST DIHERHRHRY EVNIDYKDRL 

       490        500        510        520        530        540 
EAAGLNFAGM SPDGVLPETV EYADHPWFIG VQYHPELKSR PFEPHPLFAS FIEAAIEQSR 


LV 

« Hide

References

[1]"Completion of the genome sequence of Brucella abortus and comparison to the highly similar genomes of Brucella melitensis and Brucella suis."
Halling S.M., Peterson-Burch B.D., Bricker B.J., Zuerner R.L., Qing Z., Li L.-L., Kapur V., Alt D.P., Olsen S.C.
J. Bacteriol. 187:2715-2726(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 9-941.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE017223 Genomic DNA. Translation: AAX74479.1.
RefSeqYP_221840.1. NC_006932.1.

3D structure databases

ProteinModelPortalQ57D05.
SMRQ57D05. Positions 2-540.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING262698.BruAb1_1140.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAX74479; AAX74479; BruAb1_1140.
GeneID3341150.
KEGGbmb:BruAb1_1140.
PATRIC17823970. VBIBruAbo15061_1207.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0504.
HOGENOMHOG000077515.
KOK01937.
OMAYEFNNAY.
OrthoDBEOG6RC3NR.
ProtClustDBPRK05380.

Enzyme and pathway databases

BioCycBABO262698:GJC2-1162-MONOMER.
UniPathwayUPA00159; UER00277.

Family and domain databases

Gene3D3.40.50.300. 1 hit.
HAMAPMF_01227. PyrG.
InterProIPR004468. CTP_synthase.
IPR017456. CTP_synthase_N.
IPR017926. GATASE.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERPTHR11550. PTHR11550. 1 hit.
PfamPF06418. CTP_synth_N. 1 hit.
PF00117. GATase. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
TIGRFAMsTIGR00337. PyrG. 1 hit.
PROSITEPS51273. GATASE_TYPE_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePYRG_BRUAB
AccessionPrimary (citable) accession number: Q57D05
Entry history
Integrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: May 10, 2005
Last modified: April 16, 2014
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Brucella abortus strain 9-941

Brucella abortus (strain 9-941): entries and gene names