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Protein

CTP synthase

Gene

pyrG

Organism
Brucella abortus biovar 1 (strain 9-941)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen.UniRule annotation

Catalytic activityi

ATP + UTP + L-glutamine = ADP + phosphate + CTP + L-glutamate.UniRule annotation

Enzyme regulationi

Allosterically activated by GTP, when glutamine is the substrate. Inhibited by CTP.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei380 – 3801NucleophileUniRule annotation
Active sitei514 – 5141UniRule annotation
Active sitei516 – 5161UniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. CTP synthase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. 'de novo' CTP biosynthetic process Source: UniProtKB-UniPathway
  2. glutamine metabolic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Pyrimidine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBABO262698:GJC2-1162-MONOMER.
UniPathwayiUPA00159; UER00277.

Names & Taxonomyi

Protein namesi
Recommended name:
CTP synthaseUniRule annotation (EC:6.3.4.2UniRule annotation)
Alternative name(s):
CTP synthetaseUniRule annotation
UTP--ammonia ligaseUniRule annotation
Gene namesi
Name:pyrGUniRule annotation
Ordered Locus Names:BruAb1_1140
OrganismiBrucella abortus biovar 1 (strain 9-941)
Taxonomic identifieri262698 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
ProteomesiUP000000540 Componenti: Chromosome I

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 542542CTP synthasePRO_0000266079Add
BLAST

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi262698.BruAb1_1140.

Structurei

3D structure databases

ProteinModelPortaliQ57D05.
SMRiQ57D05. Positions 2-540.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini291 – 541251Glutamine amidotransferase type-1UniRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 252252Aminator domainAdd
BLAST

Sequence similaritiesi

Belongs to the CTP synthase family.UniRule annotation
Contains 1 glutamine amidotransferase type-1 domain.UniRule annotation

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiCOG0504.
HOGENOMiHOG000077515.
KOiK01937.
OMAiYERFLNR.
OrthoDBiEOG6RC3NR.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
3.40.50.880. 1 hit.
HAMAPiMF_01227. PyrG.
InterProiIPR029062. Class_I_gatase-like.
IPR004468. CTP_synthase.
IPR017456. CTP_synthase_N.
IPR017926. GATASE.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR11550. PTHR11550. 1 hit.
PfamiPF06418. CTP_synth_N. 1 hit.
PF00117. GATase. 1 hit.
[Graphical view]
SUPFAMiSSF52317. SSF52317. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00337. PyrG. 1 hit.
PROSITEiPS51273. GATASE_TYPE_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q57D05-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARYVFITGG VVSSLGKGIA AAALAALLQA RGYRVRIRKL DPYLNVDPGT
60 70 80 90 100
MSPYQHGEVF VTDDGAETDL DLGHYERFTG RPANQQDNIT TGRIYRNIIE
110 120 130 140 150
KERRGDYLGA TVQVIPHVTD EIKNFVLEGN EDYDFVLCEI GGTVGDIEAM
160 170 180 190 200
PFLEAIRQLG NELPRGTAVY IHLTLMPYIP AAGELKTKPT QHSVKELRSI
210 220 230 240 250
GIAPDILLVR ADREIPESDR RKLSLFCNVR ESAVIQALDV ATIYDVPIAY
260 270 280 290 300
HKEGLDSEVL SAFGIDPAPK PRMDRWEEVS HRLHNPEGEV TIAVVGKYTG
310 320 330 340 350
LKDAYKSLIE ALHHGGLANK VKVNLDWIEA EVFESEDPAP YLEKVHGILV
360 370 380 390 400
PGGFGERGAE GKILAAKFAR ERKVPYFGIC FGMQMACIEA ARNLVGIEDA
410 420 430 440 450
SSSEFGPTRE PVVGLMTEWL KGNMLEKRAA AGDLGGTMRL GAYEAVLKPD
460 470 480 490 500
SKIAQIYGST DIHERHRHRY EVNIDYKDRL EAAGLNFAGM SPDGVLPETV
510 520 530 540
EYADHPWFIG VQYHPELKSR PFEPHPLFAS FIEAAIEQSR LV
Length:542
Mass (Da):60,063
Last modified:May 10, 2005 - v1
Checksum:iBBA3FDE07C633A9A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017223 Genomic DNA. Translation: AAX74479.1.
RefSeqiYP_221840.1. NC_006932.1.

Genome annotation databases

EnsemblBacteriaiAAX74479; AAX74479; BruAb1_1140.
KEGGibmb:BruAb1_1140.
PATRICi17823970. VBIBruAbo15061_1207.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017223 Genomic DNA. Translation: AAX74479.1.
RefSeqiYP_221840.1. NC_006932.1.

3D structure databases

ProteinModelPortaliQ57D05.
SMRiQ57D05. Positions 2-540.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi262698.BruAb1_1140.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAX74479; AAX74479; BruAb1_1140.
KEGGibmb:BruAb1_1140.
PATRICi17823970. VBIBruAbo15061_1207.

Phylogenomic databases

eggNOGiCOG0504.
HOGENOMiHOG000077515.
KOiK01937.
OMAiYERFLNR.
OrthoDBiEOG6RC3NR.

Enzyme and pathway databases

UniPathwayiUPA00159; UER00277.
BioCyciBABO262698:GJC2-1162-MONOMER.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
3.40.50.880. 1 hit.
HAMAPiMF_01227. PyrG.
InterProiIPR029062. Class_I_gatase-like.
IPR004468. CTP_synthase.
IPR017456. CTP_synthase_N.
IPR017926. GATASE.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR11550. PTHR11550. 1 hit.
PfamiPF06418. CTP_synth_N. 1 hit.
PF00117. GATase. 1 hit.
[Graphical view]
SUPFAMiSSF52317. SSF52317. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00337. PyrG. 1 hit.
PROSITEiPS51273. GATASE_TYPE_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Completion of the genome sequence of Brucella abortus and comparison to the highly similar genomes of Brucella melitensis and Brucella suis."
    Halling S.M., Peterson-Burch B.D., Bricker B.J., Zuerner R.L., Qing Z., Li L.-L., Kapur V., Alt D.P., Olsen S.C.
    J. Bacteriol. 187:2715-2726(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 9-941.

Entry informationi

Entry nameiPYRG_BRUAB
AccessioniPrimary (citable) accession number: Q57D05
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: May 10, 2005
Last modified: April 1, 2015
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Brucella abortus strain 9-941
    Brucella abortus (strain 9-941): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.