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Reviewed, UniProtKB/Swiss-Prot Q57CE8 (URE12_BRUAB)

Last modified November 3, 2009. Version 36. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Urease subunit alpha 2
    EC=3.5.1.5
Alternative name(s):
    Urea amidohydrolase subunit alpha 2
Gene names
Name: ureC2
Ordered Locus Names: BruAb1_1355
OrganismBrucella abortus [Complete proteome] [HAMAP]
Taxonomic identifier235 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

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Protein attributes

Sequence length573 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceInferred from homology.

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General annotation (Comments)

Catalytic activity

Urea + H2O = CO2 + 2 NH3. HAMAP MF_01953

Cofactor

Binds 2 nickel ions per subunit By similarity.

Pathway

Nitrogen metabolism; urea degradation; CO(2) and NH(3) from urea (urease route): step 1/1. HAMAP MF_01953

Subunit structure

Heterotrimer of ureA (gamma), ureB (beta) and ureC (alpha) subunits. Three heterotrimers associate to form the active enzyme By similarity.

Subcellular location

Cytoplasm By similarity.

Post-translational modification

Carbamylation allows a single lysine to coordinate two nickel ions By similarity.

Sequence similarities

Belongs to the urease family.

Contains 1 urease domain.

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Ontologies

Keywords
   Cellular componentCytoplasm
   LigandMetal-binding
Nickel
   Molecular functionHydrolase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processurea metabolic process

Inferred from electronic annotation. Source: HAMAP

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionnickel ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

urease activity

Inferred from electronic annotation. Source: HAMAP

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 573573Urease subunit alpha 2 HAMAP MF_01953
PRO_0000234140

Regions

Domain135 – 573439Urease

Sites

Active site3261Proton donor By similarity
Metal binding1401Nickel 2 By similarity
Metal binding1421Nickel 2 By similarity
Metal binding2231Nickel 1; via carbamate group By similarity
Metal binding2231Nickel 2; via carbamate group By similarity
Metal binding2521Nickel 1 By similarity
Metal binding2781Nickel 1 By similarity
Metal binding3661Nickel 2 By similarity
Binding site2251Substrate By similarity

Amino acid modifications

Modified residue2231N6-carboxylysine By similarity

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Sequences

Sequence LengthMass (Da)Tools
Q57CE8-1 [UniParc].

Last modified May 10, 2005. Version 1.
Checksum: C9A71703FF500BF6

FASTA57360,738
        10         20         30         40         50         60 
MTQISRQQYA DLYGPTIGDK IRLGDSDLYV EIEKDLRATY GDELQYGGGK TLRDGMGSEN 

        70         80         90        100        110        120 
FLTQEAGCLD LVITNVTVID AIQGVVKADV GIRNGRIVGL GKAGNPSTKD GVTRGLVTGA 

       130        140        150        160        170        180 
STDAISGEHL ILTAGGMDTH VHYIAPQQVE AALSNGITTL WGGGIGPVDG TNGVTTTNGP 

       190        200        210        220        230        240 
WNLEMMLRSI EGLPINFGIQ GKGNSTGIAP LIEQLEAGAA GFKVHEDYGA TPATIRACLS 

       250        260        270        280        290        300 
VADEYDVSVA VHTDTLNESG YVEDTIAAFD GRSVHTYHSE GAGGGHAPDL LKVVGQNNIL 

       310        320        330        340        350        360 
PSSTNPTLPC GKNSVAELFD MIMVCHNLNP KIPSDVAFAE SRVRAETIVA ESVLHDMGAI 

       370        380        390        400        410        420 
SMIGSDSQAM GRLGETFLRA IQTADAMKKA RGPLPEDAPG NDNFRVLRYI AKVTINPALT 

       430        440        450        460        470        480 
AGVGDVIGSI ESGKFADLVL WEPAFFGVKP KLVLKGGLVA WANMGDPNAS LPTPQPMYYR 

       490        500        510        520        530        540 
PMFAAYGSAL QKTSITFVSR AAYDKGVADR FGLQRLVMPV SGTRVIGKAH MVRNSYLPNI 

       550        560        570 
EVDPQTFAVK VDGVHATVKP PQSISLNQLY FFS

« Hide

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References

[1]"Completion of the genome sequence of Brucella abortus and comparison to the highly similar genomes of Brucella melitensis and Brucella suis."
Halling S.M., Peterson-Burch B.D., Bricker B.J., Zuerner R.L., Qing Z., Li L.-L., Kapur V., Alt D.P., Olsen S.C.
J. Bacteriol. 187:2715-2726(2005) [PubMed: 15805518] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 9-941 / Biovar 1.

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Cross-references

Sequence databases

AE017223 Genomic DNA. Translation: AAX74686.1.
RefSeqYP_222047.1.

3D structure databases

ModBaseSearch...

Genome annotation databases

GeneID3340381.
GenomeReviewsGene locus BruAb1_1355 in contig AE017223_GR.
KEGGbmb:BruAb1_1355.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMQ57CE8.
OMAYGINSQA.

Enzyme and pathway databases

BioCycBABO262698:BRUAB1_1355-MON.
BRENDA3.5.1.5. 575.

Family and domain databases

HAMAPMF_01953.
[Tree]
InterProIPR006680. Amidohydro_1.
IPR011612. Urease_alpha_N.
IPR005848. Urease_asu.
IPR017951. Urease_asu_c.
IPR017952. Urease_asu_core.
IPR017950. Urease_asu_CS.
[Graphical view]
PfamPF01979. Amidohydro_1. 1 hit.
PF00449. Urease_alpha. 1 hit.
[Graphical view]
PRINTSPR01752. UREASE.
TIGRFAMsTIGR01792. urease_alph. 1 hit.
PROSITEPS01120. UREASE_1. 1 hit.
PS00145. UREASE_2. 1 hit.
PS51368. UREASE_3. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameURE12_BRUAB
AccessionPrimary (citable) accession number: Q57CE8
Entry history
Integrated into UniProtKB/Swiss-Prot: May 2, 2006
Last sequence update: May 10, 2005
Last modified: November 3, 2009
This is version 36 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

Brucella abortus strain 9-941

Brucella abortus (strain 9-941): entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents