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Q57C89

- DNLJ_BRUAB

UniProt

Q57C89 - DNLJ_BRUAB

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Protein

DNA ligase

Gene

ligA

Organism
Brucella abortus biovar 1 (strain 9-941)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA.UniRule annotation

Catalytic activityi

NAD+ + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + beta-nicotinamide D-ribonucleotide + (deoxyribonucleotide)(n+m).UniRule annotation

Cofactori

Magnesium or manganese.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei126 – 1261NADUniRule annotation
Active sitei128 – 1281N6-AMP-lysine intermediateUniRule annotation
Binding sitei149 – 1491NADUniRule annotation
Binding sitei185 – 1851NADUniRule annotation
Binding sitei301 – 3011NADUniRule annotation
Binding sitei325 – 3251NADUniRule annotation
Metal bindingi430 – 4301ZincUniRule annotation
Metal bindingi433 – 4331ZincUniRule annotation
Metal bindingi448 – 4481ZincUniRule annotation
Metal bindingi454 – 4541ZincUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi42 – 465NADUniRule annotation
Nucleotide bindingi92 – 932NADUniRule annotation

GO - Molecular functioni

  1. DNA binding Source: InterPro
  2. DNA ligase (NAD+) activity Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. DNA repair Source: UniProtKB-KW
  2. DNA replication Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

DNA damage, DNA repair, DNA replication

Keywords - Ligandi

Magnesium, Manganese, Metal-binding, NAD, Zinc

Enzyme and pathway databases

BioCyciBABO262698:GJC2-1445-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA ligaseUniRule annotation (EC:6.5.1.2UniRule annotation)
Alternative name(s):
Polydeoxyribonucleotide synthase [NAD(+)]UniRule annotation
Gene namesi
Name:ligAUniRule annotation
Ordered Locus Names:BruAb1_1415
OrganismiBrucella abortus biovar 1 (strain 9-941)
Taxonomic identifieri262698 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
ProteomesiUP000000540: Chromosome I

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 719719DNA ligasePRO_0000313152Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi262698.BruAb1_1415.

Structurei

3D structure databases

ProteinModelPortaliQ57C89.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini640 – 71980BRCTUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the NAD-dependent DNA ligase family. LigA subfamily.UniRule annotation
Contains 1 BRCT domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG0272.
HOGENOMiHOG000218459.
KOiK01972.
OMAiKEYYVDS.
OrthoDBiEOG6TTVM9.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
3.40.50.10190. 1 hit.
HAMAPiMF_01588. DNA_ligase_A.
InterProiIPR001357. BRCT_dom.
IPR018239. DNA_ligase_AS.
IPR001679. DNAligase.
IPR013839. DNAligase_adenylation.
IPR013840. DNAligase_N.
IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif.
IPR012340. NA-bd_OB-fold.
IPR004150. NAD_DNA_ligase_OB.
IPR010994. RuvA_2-like.
IPR004149. Znf_DNAligase_C4.
[Graphical view]
PfamiPF00533. BRCT. 1 hit.
PF01653. DNA_ligase_aden. 1 hit.
PF03120. DNA_ligase_OB. 1 hit.
PF03119. DNA_ligase_ZBD. 1 hit.
[Graphical view]
PIRSFiPIRSF001604. LigA. 1 hit.
SMARTiSM00292. BRCT. 1 hit.
SM00278. HhH1. 2 hits.
SM00532. LIGANc. 1 hit.
[Graphical view]
SUPFAMiSSF47781. SSF47781. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF52113. SSF52113. 1 hit.
TIGRFAMsiTIGR00575. dnlj. 1 hit.
PROSITEiPS50172. BRCT. 1 hit.
PS01055. DNA_LIGASE_N1. 1 hit.
PS01056. DNA_LIGASE_N2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q57C89-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSDISVEKLT ELEAAAELER LARAIAHHDE LYHAKDRPEI SDAAYDALKR
60 70 80 90 100
RNEAIEAHFP ALVRDDSPSR RVGAAPALAT FAPVVHARPM LSLDNAFSDE
110 120 130 140 150
DVRDFVGSVY RFLGQLPDDS IAFTAEPKID GLSMSIRYEN GILVSGATRG
160 170 180 190 200
DGTTGENVTA NIRTIAEIPN RLPAGAPAVV EVRGEVYMAK SDFLTLNAQM
210 220 230 240 250
EAEGKQTYVN PRNTAAGSLR QLDAKVTASR KLRFFAYAWG EMSDMPADTQ
260 270 280 290 300
LGMVEVFRQW GFPVNPLMKR FNSVDGLLAH YRAIGMERPT LDYDIDGVVY
310 320 330 340 350
KVDRLDLQTR LGFRSRSPRW AIAHKFPAEQ ALTILRGIDI QVGRTGALTP
360 370 380 390 400
VARLEPITVG GVVVTNATLH NEDYIKGIGQ KGEPIREGRD IRIGDSVIVQ
410 420 430 440 450
RAGDVIPQIV DVVLEEGKKR GEPYQFPHVC PACGSHAVRE EGEAVRRCTG
460 470 480 490 500
GLICPAQAVE RIRHFVSRNA FDIEGLGEKQ VEFFFNAEDP ALCIRSPADI
510 520 530 540 550
FTLKKRQENS LTKLQNIEGF GATSVKKLYD AIDARREIAL HRFLFGLGIR
560 570 580 590 600
HVGEVNAKRL ARAYLSYAAF EKAALEAVPP KEGDRTDKGS EAWQDMLAVE
610 620 630 640 650
GIGSIVAEAV VDFYGEPHNR EVLAALLAEV TPLDEEARVA TGSPVEGKTV
660 670 680 690 700
VFTGSLERMS RDEAKAMAER HGAKTAGSVS KKTDLVVAGP GAGSKLAKAT
710
ELGIEVINED DWFKLVGED
Length:719
Mass (Da):78,681
Last modified:May 10, 2005 - v1
Checksum:i122949D2FB8B1B79
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE017223 Genomic DNA. Translation: AAX74745.1.
RefSeqiYP_222106.1. NC_006932.1.

Genome annotation databases

EnsemblBacteriaiAAX74745; AAX74745; BruAb1_1415.
GeneIDi3340487.
KEGGibmb:BruAb1_1415.
PATRICi17824565. VBIBruAbo15061_1498.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE017223 Genomic DNA. Translation: AAX74745.1 .
RefSeqi YP_222106.1. NC_006932.1.

3D structure databases

ProteinModelPortali Q57C89.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 262698.BruAb1_1415.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAX74745 ; AAX74745 ; BruAb1_1415 .
GeneIDi 3340487.
KEGGi bmb:BruAb1_1415.
PATRICi 17824565. VBIBruAbo15061_1498.

Phylogenomic databases

eggNOGi COG0272.
HOGENOMi HOG000218459.
KOi K01972.
OMAi KEYYVDS.
OrthoDBi EOG6TTVM9.

Enzyme and pathway databases

BioCyci BABO262698:GJC2-1445-MONOMER.

Family and domain databases

Gene3Di 2.40.50.140. 1 hit.
3.40.50.10190. 1 hit.
HAMAPi MF_01588. DNA_ligase_A.
InterProi IPR001357. BRCT_dom.
IPR018239. DNA_ligase_AS.
IPR001679. DNAligase.
IPR013839. DNAligase_adenylation.
IPR013840. DNAligase_N.
IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif.
IPR012340. NA-bd_OB-fold.
IPR004150. NAD_DNA_ligase_OB.
IPR010994. RuvA_2-like.
IPR004149. Znf_DNAligase_C4.
[Graphical view ]
Pfami PF00533. BRCT. 1 hit.
PF01653. DNA_ligase_aden. 1 hit.
PF03120. DNA_ligase_OB. 1 hit.
PF03119. DNA_ligase_ZBD. 1 hit.
[Graphical view ]
PIRSFi PIRSF001604. LigA. 1 hit.
SMARTi SM00292. BRCT. 1 hit.
SM00278. HhH1. 2 hits.
SM00532. LIGANc. 1 hit.
[Graphical view ]
SUPFAMi SSF47781. SSF47781. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF52113. SSF52113. 1 hit.
TIGRFAMsi TIGR00575. dnlj. 1 hit.
PROSITEi PS50172. BRCT. 1 hit.
PS01055. DNA_LIGASE_N1. 1 hit.
PS01056. DNA_LIGASE_N2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Completion of the genome sequence of Brucella abortus and comparison to the highly similar genomes of Brucella melitensis and Brucella suis."
    Halling S.M., Peterson-Burch B.D., Bricker B.J., Zuerner R.L., Qing Z., Li L.-L., Kapur V., Alt D.P., Olsen S.C.
    J. Bacteriol. 187:2715-2726(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 9-941.

Entry informationi

Entry nameiDNLJ_BRUAB
AccessioniPrimary (citable) accession number: Q57C89
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: May 10, 2005
Last modified: October 1, 2014
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Brucella abortus strain 9-941
    Brucella abortus (strain 9-941): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3