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Q57C89

- DNLJ_BRUAB

UniProt

Q57C89 - DNLJ_BRUAB

Protein

DNA ligase

Gene

ligA

Organism
Brucella abortus biovar 1 (strain 9-941)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 69 (01 Oct 2014)
      Sequence version 1 (10 May 2005)
      Previous versions | rss
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    Functioni

    DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA.UniRule annotation

    Catalytic activityi

    NAD+ + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + beta-nicotinamide D-ribonucleotide + (deoxyribonucleotide)(n+m).UniRule annotation

    Cofactori

    Magnesium or manganese.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei126 – 1261NADUniRule annotation
    Active sitei128 – 1281N6-AMP-lysine intermediateUniRule annotation
    Binding sitei149 – 1491NADUniRule annotation
    Binding sitei185 – 1851NADUniRule annotation
    Binding sitei301 – 3011NADUniRule annotation
    Binding sitei325 – 3251NADUniRule annotation
    Metal bindingi430 – 4301ZincUniRule annotation
    Metal bindingi433 – 4331ZincUniRule annotation
    Metal bindingi448 – 4481ZincUniRule annotation
    Metal bindingi454 – 4541ZincUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi42 – 465NADUniRule annotation
    Nucleotide bindingi92 – 932NADUniRule annotation

    GO - Molecular functioni

    1. DNA binding Source: InterPro
    2. DNA ligase (NAD+) activity Source: UniProtKB-HAMAP
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. DNA repair Source: UniProtKB-KW
    2. DNA replication Source: UniProtKB-KW

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    DNA damage, DNA repair, DNA replication

    Keywords - Ligandi

    Magnesium, Manganese, Metal-binding, NAD, Zinc

    Enzyme and pathway databases

    BioCyciBABO262698:GJC2-1445-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA ligaseUniRule annotation (EC:6.5.1.2UniRule annotation)
    Alternative name(s):
    Polydeoxyribonucleotide synthase [NAD(+)]UniRule annotation
    Gene namesi
    Name:ligAUniRule annotation
    Ordered Locus Names:BruAb1_1415
    OrganismiBrucella abortus biovar 1 (strain 9-941)
    Taxonomic identifieri262698 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
    ProteomesiUP000000540: Chromosome I

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 719719DNA ligasePRO_0000313152Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi262698.BruAb1_1415.

    Structurei

    3D structure databases

    ProteinModelPortaliQ57C89.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini640 – 71980BRCTUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the NAD-dependent DNA ligase family. LigA subfamily.UniRule annotation
    Contains 1 BRCT domain.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0272.
    HOGENOMiHOG000218459.
    KOiK01972.
    OMAiKEYYVDS.
    OrthoDBiEOG6TTVM9.

    Family and domain databases

    Gene3Di2.40.50.140. 1 hit.
    3.40.50.10190. 1 hit.
    HAMAPiMF_01588. DNA_ligase_A.
    InterProiIPR001357. BRCT_dom.
    IPR018239. DNA_ligase_AS.
    IPR001679. DNAligase.
    IPR013839. DNAligase_adenylation.
    IPR013840. DNAligase_N.
    IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif.
    IPR012340. NA-bd_OB-fold.
    IPR004150. NAD_DNA_ligase_OB.
    IPR010994. RuvA_2-like.
    IPR004149. Znf_DNAligase_C4.
    [Graphical view]
    PfamiPF00533. BRCT. 1 hit.
    PF01653. DNA_ligase_aden. 1 hit.
    PF03120. DNA_ligase_OB. 1 hit.
    PF03119. DNA_ligase_ZBD. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001604. LigA. 1 hit.
    SMARTiSM00292. BRCT. 1 hit.
    SM00278. HhH1. 2 hits.
    SM00532. LIGANc. 1 hit.
    [Graphical view]
    SUPFAMiSSF47781. SSF47781. 1 hit.
    SSF50249. SSF50249. 1 hit.
    SSF52113. SSF52113. 1 hit.
    TIGRFAMsiTIGR00575. dnlj. 1 hit.
    PROSITEiPS50172. BRCT. 1 hit.
    PS01055. DNA_LIGASE_N1. 1 hit.
    PS01056. DNA_LIGASE_N2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q57C89-1 [UniParc]FASTAAdd to Basket

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    MSDISVEKLT ELEAAAELER LARAIAHHDE LYHAKDRPEI SDAAYDALKR    50
    RNEAIEAHFP ALVRDDSPSR RVGAAPALAT FAPVVHARPM LSLDNAFSDE 100
    DVRDFVGSVY RFLGQLPDDS IAFTAEPKID GLSMSIRYEN GILVSGATRG 150
    DGTTGENVTA NIRTIAEIPN RLPAGAPAVV EVRGEVYMAK SDFLTLNAQM 200
    EAEGKQTYVN PRNTAAGSLR QLDAKVTASR KLRFFAYAWG EMSDMPADTQ 250
    LGMVEVFRQW GFPVNPLMKR FNSVDGLLAH YRAIGMERPT LDYDIDGVVY 300
    KVDRLDLQTR LGFRSRSPRW AIAHKFPAEQ ALTILRGIDI QVGRTGALTP 350
    VARLEPITVG GVVVTNATLH NEDYIKGIGQ KGEPIREGRD IRIGDSVIVQ 400
    RAGDVIPQIV DVVLEEGKKR GEPYQFPHVC PACGSHAVRE EGEAVRRCTG 450
    GLICPAQAVE RIRHFVSRNA FDIEGLGEKQ VEFFFNAEDP ALCIRSPADI 500
    FTLKKRQENS LTKLQNIEGF GATSVKKLYD AIDARREIAL HRFLFGLGIR 550
    HVGEVNAKRL ARAYLSYAAF EKAALEAVPP KEGDRTDKGS EAWQDMLAVE 600
    GIGSIVAEAV VDFYGEPHNR EVLAALLAEV TPLDEEARVA TGSPVEGKTV 650
    VFTGSLERMS RDEAKAMAER HGAKTAGSVS KKTDLVVAGP GAGSKLAKAT 700
    ELGIEVINED DWFKLVGED 719
    Length:719
    Mass (Da):78,681
    Last modified:May 10, 2005 - v1
    Checksum:i122949D2FB8B1B79
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE017223 Genomic DNA. Translation: AAX74745.1.
    RefSeqiYP_222106.1. NC_006932.1.

    Genome annotation databases

    EnsemblBacteriaiAAX74745; AAX74745; BruAb1_1415.
    GeneIDi3340487.
    KEGGibmb:BruAb1_1415.
    PATRICi17824565. VBIBruAbo15061_1498.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE017223 Genomic DNA. Translation: AAX74745.1 .
    RefSeqi YP_222106.1. NC_006932.1.

    3D structure databases

    ProteinModelPortali Q57C89.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 262698.BruAb1_1415.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAX74745 ; AAX74745 ; BruAb1_1415 .
    GeneIDi 3340487.
    KEGGi bmb:BruAb1_1415.
    PATRICi 17824565. VBIBruAbo15061_1498.

    Phylogenomic databases

    eggNOGi COG0272.
    HOGENOMi HOG000218459.
    KOi K01972.
    OMAi KEYYVDS.
    OrthoDBi EOG6TTVM9.

    Enzyme and pathway databases

    BioCyci BABO262698:GJC2-1445-MONOMER.

    Family and domain databases

    Gene3Di 2.40.50.140. 1 hit.
    3.40.50.10190. 1 hit.
    HAMAPi MF_01588. DNA_ligase_A.
    InterProi IPR001357. BRCT_dom.
    IPR018239. DNA_ligase_AS.
    IPR001679. DNAligase.
    IPR013839. DNAligase_adenylation.
    IPR013840. DNAligase_N.
    IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif.
    IPR012340. NA-bd_OB-fold.
    IPR004150. NAD_DNA_ligase_OB.
    IPR010994. RuvA_2-like.
    IPR004149. Znf_DNAligase_C4.
    [Graphical view ]
    Pfami PF00533. BRCT. 1 hit.
    PF01653. DNA_ligase_aden. 1 hit.
    PF03120. DNA_ligase_OB. 1 hit.
    PF03119. DNA_ligase_ZBD. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001604. LigA. 1 hit.
    SMARTi SM00292. BRCT. 1 hit.
    SM00278. HhH1. 2 hits.
    SM00532. LIGANc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47781. SSF47781. 1 hit.
    SSF50249. SSF50249. 1 hit.
    SSF52113. SSF52113. 1 hit.
    TIGRFAMsi TIGR00575. dnlj. 1 hit.
    PROSITEi PS50172. BRCT. 1 hit.
    PS01055. DNA_LIGASE_N1. 1 hit.
    PS01056. DNA_LIGASE_N2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Completion of the genome sequence of Brucella abortus and comparison to the highly similar genomes of Brucella melitensis and Brucella suis."
      Halling S.M., Peterson-Burch B.D., Bricker B.J., Zuerner R.L., Qing Z., Li L.-L., Kapur V., Alt D.P., Olsen S.C.
      J. Bacteriol. 187:2715-2726(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 9-941.

    Entry informationi

    Entry nameiDNLJ_BRUAB
    AccessioniPrimary (citable) accession number: Q57C89
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: May 10, 2005
    Last modified: October 1, 2014
    This is version 69 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Brucella abortus strain 9-941
      Brucella abortus (strain 9-941): entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3