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Protein

DNA ligase

Gene

ligA

Organism
Brucella abortus biovar 1 (strain 9-941)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA.UniRule annotation

Catalytic activityi

NAD+ + (deoxyribonucleotide)(n)-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)(m) = (deoxyribonucleotide)(n+m) + AMP + beta-nicotinamide D-nucleotide.UniRule annotation

Cofactori

Mg2+UniRule annotation, Mn2+UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei126NADUniRule annotation1
Active sitei128N6-AMP-lysine intermediateUniRule annotation1
Binding sitei149NADUniRule annotation1
Binding sitei185NADUniRule annotation1
Binding sitei301NADUniRule annotation1
Binding sitei325NADUniRule annotation1
Metal bindingi430ZincUniRule annotation1
Metal bindingi433ZincUniRule annotation1
Metal bindingi448ZincUniRule annotation1
Metal bindingi454ZincUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi42 – 46NADUniRule annotation5
Nucleotide bindingi92 – 93NADUniRule annotation2

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processDNA damage, DNA repair, DNA replication
LigandMagnesium, Manganese, Metal-binding, NAD, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
DNA ligaseUniRule annotation (EC:6.5.1.2UniRule annotation)
Alternative name(s):
Polydeoxyribonucleotide synthase [NAD(+)]UniRule annotation
Gene namesi
Name:ligAUniRule annotation
Ordered Locus Names:BruAb1_1415
OrganismiBrucella abortus biovar 1 (strain 9-941)
Taxonomic identifieri262698 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
Proteomesi
  • UP000000540 Componenti: Chromosome I

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003131521 – 719DNA ligaseAdd BLAST719

Proteomic databases

PRIDEiQ57C89

Structurei

3D structure databases

ProteinModelPortaliQ57C89
SMRiQ57C89
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini640 – 719BRCTUniRule annotationAdd BLAST80

Sequence similaritiesi

Belongs to the NAD-dependent DNA ligase family. LigA subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000218459
KOiK01972
OMAiFTAKSPR

Family and domain databases

CDDicd00027 BRCT, 1 hit
cd00114 LIGANc, 1 hit
Gene3Di3.40.50.10190, 1 hit
HAMAPiMF_01588 DNA_ligase_A, 1 hit
InterProiView protein in InterPro
IPR001357 BRCT_dom
IPR036420 BRCT_dom_sf
IPR018239 DNA_ligase_AS
IPR033136 DNA_ligase_CS
IPR001679 DNAligase
IPR013839 DNAligase_adenylation
IPR013840 DNAligase_N
IPR012340 NA-bd_OB-fold
IPR004150 NAD_DNA_ligase_OB
IPR010994 RuvA_2-like
IPR004149 Znf_DNAligase_C4
PfamiView protein in Pfam
PF00533 BRCT, 1 hit
PF01653 DNA_ligase_aden, 1 hit
PF03120 DNA_ligase_OB, 1 hit
PF03119 DNA_ligase_ZBD, 1 hit
PIRSFiPIRSF001604 LigA, 1 hit
SMARTiView protein in SMART
SM00292 BRCT, 1 hit
SM00532 LIGANc, 1 hit
SUPFAMiSSF47781 SSF47781, 1 hit
SSF50249 SSF50249, 1 hit
SSF52113 SSF52113, 1 hit
TIGRFAMsiTIGR00575 dnlj, 1 hit
PROSITEiView protein in PROSITE
PS50172 BRCT, 1 hit
PS01055 DNA_LIGASE_N1, 1 hit
PS01056 DNA_LIGASE_N2, 1 hit

Sequencei

Sequence statusi: Complete.

Q57C89-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDISVEKLT ELEAAAELER LARAIAHHDE LYHAKDRPEI SDAAYDALKR
60 70 80 90 100
RNEAIEAHFP ALVRDDSPSR RVGAAPALAT FAPVVHARPM LSLDNAFSDE
110 120 130 140 150
DVRDFVGSVY RFLGQLPDDS IAFTAEPKID GLSMSIRYEN GILVSGATRG
160 170 180 190 200
DGTTGENVTA NIRTIAEIPN RLPAGAPAVV EVRGEVYMAK SDFLTLNAQM
210 220 230 240 250
EAEGKQTYVN PRNTAAGSLR QLDAKVTASR KLRFFAYAWG EMSDMPADTQ
260 270 280 290 300
LGMVEVFRQW GFPVNPLMKR FNSVDGLLAH YRAIGMERPT LDYDIDGVVY
310 320 330 340 350
KVDRLDLQTR LGFRSRSPRW AIAHKFPAEQ ALTILRGIDI QVGRTGALTP
360 370 380 390 400
VARLEPITVG GVVVTNATLH NEDYIKGIGQ KGEPIREGRD IRIGDSVIVQ
410 420 430 440 450
RAGDVIPQIV DVVLEEGKKR GEPYQFPHVC PACGSHAVRE EGEAVRRCTG
460 470 480 490 500
GLICPAQAVE RIRHFVSRNA FDIEGLGEKQ VEFFFNAEDP ALCIRSPADI
510 520 530 540 550
FTLKKRQENS LTKLQNIEGF GATSVKKLYD AIDARREIAL HRFLFGLGIR
560 570 580 590 600
HVGEVNAKRL ARAYLSYAAF EKAALEAVPP KEGDRTDKGS EAWQDMLAVE
610 620 630 640 650
GIGSIVAEAV VDFYGEPHNR EVLAALLAEV TPLDEEARVA TGSPVEGKTV
660 670 680 690 700
VFTGSLERMS RDEAKAMAER HGAKTAGSVS KKTDLVVAGP GAGSKLAKAT
710
ELGIEVINED DWFKLVGED
Length:719
Mass (Da):78,681
Last modified:May 10, 2005 - v1
Checksum:i122949D2FB8B1B79
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017223 Genomic DNA Translation: AAX74745.1
RefSeqiWP_002964528.1, NC_006932.1

Genome annotation databases

EnsemblBacteriaiAAX74745; AAX74745; BruAb1_1415
KEGGibmb:BruAb1_1415

Similar proteinsi

Entry informationi

Entry nameiDNLJ_BRUAB
AccessioniPrimary (citable) accession number: Q57C89
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: May 10, 2005
Last modified: May 23, 2018
This is version 88 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

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