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Protein

Oxygen-dependent coproporphyrinogen-III oxidase

Gene

hemF

Organism
Brucella abortus biovar 1 (strain 9-941)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the heme biosynthesis. Catalyzes the aerobic oxidative decarboxylation of propionate groups of rings A and B of coproporphyrinogen-III to yield the vinyl groups in protoporphyrinogen-IX.UniRule annotation

Catalytic activityi

Coproporphyrinogen-III + O2 + 2 H+ = protoporphyrinogen-IX + 2 CO2 + 2 H2O.UniRule annotation

Cofactori

a divalent metal cationUniRule annotation

Pathway:iprotoporphyrin-IX biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes protoporphyrinogen-IX from coproporphyrinogen-III (O2 route).UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Oxygen-dependent coproporphyrinogen-III oxidase (hemF)
This subpathway is part of the pathway protoporphyrin-IX biosynthesis, which is itself part of Porphyrin-containing compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes protoporphyrinogen-IX from coproporphyrinogen-III (O2 route), the pathway protoporphyrin-IX biosynthesis and in Porphyrin-containing compound metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei116 – 1161SubstrateUniRule annotation
Metal bindingi120 – 1201Divalent cationUniRule annotation
Active sitei130 – 1301Proton donorUniRule annotation
Metal bindingi130 – 1301Divalent cationUniRule annotation
Metal bindingi168 – 1681Divalent cationUniRule annotation
Metal bindingi199 – 1991Divalent cationUniRule annotation
Sitei199 – 1991Important for dimerizationUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Heme biosynthesis, Porphyrin biosynthesis

Keywords - Ligandi

Metal-binding

Enzyme and pathway databases

BioCyciBABO262698:GJC2-1570-MONOMER.
UniPathwayiUPA00251; UER00322.

Names & Taxonomyi

Protein namesi
Recommended name:
Oxygen-dependent coproporphyrinogen-III oxidaseUniRule annotation (EC:1.3.3.3UniRule annotation)
Short name:
CPOUniRule annotation
Short name:
Coprogen oxidaseUniRule annotation
Short name:
CoproporphyrinogenaseUniRule annotation
Gene namesi
Name:hemFUniRule annotation
Ordered Locus Names:BruAb1_1539
OrganismiBrucella abortus biovar 1 (strain 9-941)
Taxonomic identifieri262698 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
ProteomesiUP000000540 Componenti: Chromosome I

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 303303Oxygen-dependent coproporphyrinogen-III oxidasePRO_1000119788Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ57BW7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni132 – 1343Substrate bindingUniRule annotation
Regioni268 – 30336Important for dimerizationUniRule annotationAdd
BLAST
Regioni286 – 2883Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the aerobic coproporphyrinogen-III oxidase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0408.
HOGENOMiHOG000262768.
KOiK00228.
OMAiVREREFQ.
OrthoDBiEOG67T5GJ.

Family and domain databases

Gene3Di3.40.1500.10. 1 hit.
HAMAPiMF_00333. Coprogen_oxidas.
InterProiIPR001260. Coprogen_oxidase_aer.
IPR018375. Coprogen_oxidase_CS.
[Graphical view]
PANTHERiPTHR10755. PTHR10755. 1 hit.
PfamiPF01218. Coprogen_oxidas. 1 hit.
[Graphical view]
PIRSFiPIRSF000166. Coproporphyri_ox. 1 hit.
PRINTSiPR00073. COPRGNOXDASE.
SUPFAMiSSF102886. SSF102886. 1 hit.
PROSITEiPS01021. COPROGEN_OXIDASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q57BW7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKREDIPAII PADIEEKKKA AQSWFEELRD RICASYEQLE DELQGPLSDR
60 70 80 90 100
EPGRFVRTPW QKDDGNGGGV MSIMHGRVFE KVGVHVSTVH GEFSPEFRKQ
110 120 130 140 150
IPGAEEDPRY WASGISLIAH PQNPNVPAVH MNTRMIVTTR QWFAGGADLT
160 170 180 190 200
PVLDRRRTQE DPDTLAFHKA FRFICEKHKD IVDYQRLKEW CDEYFFLPHR
210 220 230 240 250
DEPRGIGGIF YDWLHSPEEK GGWDSDFAFT RDVGRGFSVV YPHLVRQNFN
260 270 280 290 300
KDWTEADRDE QLIRRGRYVE FNLLYDRGTI FGLKTGGNMN AILSSMPPVV

KWP
Length:303
Mass (Da):35,111
Last modified:May 10, 2005 - v1
Checksum:i65C4C1B754D13CE0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017223 Genomic DNA. Translation: AAX74867.1.
RefSeqiWP_002964654.1. NC_006932.1.

Genome annotation databases

EnsemblBacteriaiAAX74867; AAX74867; BruAb1_1539.
GeneIDi3788746.
KEGGibmb:BruAb1_1539.
PATRICi17824828. VBIBruAbo15061_1628.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017223 Genomic DNA. Translation: AAX74867.1.
RefSeqiWP_002964654.1. NC_006932.1.

3D structure databases

ProteinModelPortaliQ57BW7.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAX74867; AAX74867; BruAb1_1539.
GeneIDi3788746.
KEGGibmb:BruAb1_1539.
PATRICi17824828. VBIBruAbo15061_1628.

Phylogenomic databases

eggNOGiCOG0408.
HOGENOMiHOG000262768.
KOiK00228.
OMAiVREREFQ.
OrthoDBiEOG67T5GJ.

Enzyme and pathway databases

UniPathwayiUPA00251; UER00322.
BioCyciBABO262698:GJC2-1570-MONOMER.

Family and domain databases

Gene3Di3.40.1500.10. 1 hit.
HAMAPiMF_00333. Coprogen_oxidas.
InterProiIPR001260. Coprogen_oxidase_aer.
IPR018375. Coprogen_oxidase_CS.
[Graphical view]
PANTHERiPTHR10755. PTHR10755. 1 hit.
PfamiPF01218. Coprogen_oxidas. 1 hit.
[Graphical view]
PIRSFiPIRSF000166. Coproporphyri_ox. 1 hit.
PRINTSiPR00073. COPRGNOXDASE.
SUPFAMiSSF102886. SSF102886. 1 hit.
PROSITEiPS01021. COPROGEN_OXIDASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Completion of the genome sequence of Brucella abortus and comparison to the highly similar genomes of Brucella melitensis and Brucella suis."
    Halling S.M., Peterson-Burch B.D., Bricker B.J., Zuerner R.L., Qing Z., Li L.-L., Kapur V., Alt D.P., Olsen S.C.
    J. Bacteriol. 187:2715-2726(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 9-941.

Entry informationi

Entry nameiHEM6_BRUAB
AccessioniPrimary (citable) accession number: Q57BW7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: May 10, 2005
Last modified: July 22, 2015
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Brucella abortus strain 9-941
    Brucella abortus (strain 9-941): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.