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Q57BM8

- MASZ_BRUAB

UniProt

Q57BM8 - MASZ_BRUAB

Protein

Malate synthase G

Gene

glcB

Organism
Brucella abortus biovar 1 (strain 9-941)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 58 (01 Oct 2014)
      Sequence version 1 (10 May 2005)
      Previous versions | rss
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    Functioni

    Involved in the glycolate utilization. Catalyzes the condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl-CoA) and glyoxylate to form malate and CoA.UniRule annotation

    Catalytic activityi

    Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.UniRule annotation

    Cofactori

    Magnesium.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei123 – 1231Acetyl-CoA; via carbonyl oxygenUniRule annotation
    Binding sitei281 – 2811Acetyl-CoAUniRule annotation
    Binding sitei318 – 3181Acetyl-CoAUniRule annotation
    Active sitei345 – 3451Proton acceptorUniRule annotation
    Binding sitei345 – 3451GlyoxylateUniRule annotation
    Metal bindingi437 – 4371MagnesiumUniRule annotation
    Binding sitei437 – 4371GlyoxylateUniRule annotation
    Metal bindingi465 – 4651MagnesiumUniRule annotation
    Binding sitei546 – 5461Acetyl-CoA; via carbonyl oxygenUniRule annotation
    Active sitei636 – 6361Proton donorUniRule annotation

    GO - Molecular functioni

    1. malate synthase activity Source: UniProtKB-HAMAP
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. glyoxylate cycle Source: UniProtKB-HAMAP
    2. tricarboxylic acid cycle Source: UniProtKB-KW

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Glyoxylate bypass, Tricarboxylic acid cycle

    Keywords - Ligandi

    Magnesium, Metal-binding

    Enzyme and pathway databases

    BioCyciBABO262698:GJC2-1675-MONOMER.
    UniPathwayiUPA00703; UER00720.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Malate synthase GUniRule annotation (EC:2.3.3.9UniRule annotation)
    Gene namesi
    Name:glcBUniRule annotation
    Ordered Locus Names:BruAb1_1636
    OrganismiBrucella abortus biovar 1 (strain 9-941)
    Taxonomic identifieri262698 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
    ProteomesiUP000000540: Chromosome I

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 728728Malate synthase GPRO_1000056898Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei622 – 6221Cysteine sulfenic acid (-SOH)UniRule annotation

    Keywords - PTMi

    Oxidation

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi262698.BruAb1_1636.

    Structurei

    3D structure databases

    ProteinModelPortaliQ57BM8.
    SMRiQ57BM8. Positions 10-725.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni130 – 1312Acetyl-CoA bindingUniRule annotation
    Regioni462 – 4654Glyoxylate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the malate synthase family. GlcB subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG2225.
    HOGENOMiHOG000220740.
    KOiK01638.
    OMAiARTENVD.
    OrthoDBiEOG6HJ286.

    Family and domain databases

    Gene3Di2.170.170.11. 2 hits.
    HAMAPiMF_00641. Malate_synth_G.
    InterProiIPR011076. Malate_synth-like.
    IPR023310. Malate_synth_G_beta_sub_dom.
    IPR001465. Malate_synthase.
    IPR006253. Malate_synthG.
    [Graphical view]
    PfamiPF01274. Malate_synthase. 1 hit.
    [Graphical view]
    SUPFAMiSSF51645. SSF51645. 1 hit.
    TIGRFAMsiTIGR01345. malate_syn_G. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q57BM8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGSAEKRNYV EIEGLAVAPE LVEFLAKEAA PGTGVEPEKF WKGFAAIIRD    50
    LTPKNRALLA KRDELQARID AWYKENRDKG YSQADYQQFL KDIGYLLPEG 100
    GAFSVSTTNV DPEITHIAGP QLVVPVMNAR YALNAANARW GSLYDALYGT 150
    DAISEADGAE KGKGYNPKRG EKVIAWAKNF LDESAPLSTG KWADVAGLAV 200
    NDGKLEIRLT DGSATTLKDE SQFKGYNGDA ASPTNVLLAK HNMHVDIVIN 250
    ADHPIGKTDP AHIADVVLES AISTIQDCED SIAVVDAEDK VAVYRNWLGL 300
    MNGKLEDTFE KNGKQMTRRL NGDRTYTAPD GSTLTLKGRS LMLVRNVGHL 350
    MTNPAILDAE GNEVPEGIMD AAFTSLIALH DIGPNGRHMN SREGSVYIVK 400
    PKMHGPEEVA FANEIFTRTE EMLGMKPNTL KIGIMDEERR TTVNLKEAIR 450
    AAKDRVVFIN TGFLDRTGDE IHTSMEAGPM IRKGDMKQAA WIGAYEQWNV 500
    DIGLECGLSG HAQIGKGMWA MPDMMAAMLE QKIAHPKAGA NTAWVPSPTA 550
    ATLHATHYHK IDVAAVQEKL KSRPRAKLDD ILSVPVAVRP NWTPDDIQHE 600
    IDNNAQGILG YVVRWIDQGV GCSKVPDINN VGLMEDRATL RISAQHIANW 650
    LYHGVVSEAQ VMETMKRMAA IVDKQNEGDP LYRPMAADFD KSIAFQAACD 700
    LVFKGREQPN GYTEPVLHRR RLELKQAS 728
    Length:728
    Mass (Da):80,044
    Last modified:May 10, 2005 - v1
    Checksum:iA6301C27C4FF27FA
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE017223 Genomic DNA. Translation: AAX74956.1.
    RefSeqiYP_222317.1. NC_006932.1.

    Genome annotation databases

    EnsemblBacteriaiAAX74956; AAX74956; BruAb1_1636.
    GeneIDi3340828.
    KEGGibmb:BruAb1_1636.
    PATRICi17825050. VBIBruAbo15061_1728.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE017223 Genomic DNA. Translation: AAX74956.1 .
    RefSeqi YP_222317.1. NC_006932.1.

    3D structure databases

    ProteinModelPortali Q57BM8.
    SMRi Q57BM8. Positions 10-725.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 262698.BruAb1_1636.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAX74956 ; AAX74956 ; BruAb1_1636 .
    GeneIDi 3340828.
    KEGGi bmb:BruAb1_1636.
    PATRICi 17825050. VBIBruAbo15061_1728.

    Phylogenomic databases

    eggNOGi COG2225.
    HOGENOMi HOG000220740.
    KOi K01638.
    OMAi ARTENVD.
    OrthoDBi EOG6HJ286.

    Enzyme and pathway databases

    UniPathwayi UPA00703 ; UER00720 .
    BioCyci BABO262698:GJC2-1675-MONOMER.

    Family and domain databases

    Gene3Di 2.170.170.11. 2 hits.
    HAMAPi MF_00641. Malate_synth_G.
    InterProi IPR011076. Malate_synth-like.
    IPR023310. Malate_synth_G_beta_sub_dom.
    IPR001465. Malate_synthase.
    IPR006253. Malate_synthG.
    [Graphical view ]
    Pfami PF01274. Malate_synthase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51645. SSF51645. 1 hit.
    TIGRFAMsi TIGR01345. malate_syn_G. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Completion of the genome sequence of Brucella abortus and comparison to the highly similar genomes of Brucella melitensis and Brucella suis."
      Halling S.M., Peterson-Burch B.D., Bricker B.J., Zuerner R.L., Qing Z., Li L.-L., Kapur V., Alt D.P., Olsen S.C.
      J. Bacteriol. 187:2715-2726(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 9-941.

    Entry informationi

    Entry nameiMASZ_BRUAB
    AccessioniPrimary (citable) accession number: Q57BM8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: May 10, 2005
    Last modified: October 1, 2014
    This is version 58 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Brucella abortus strain 9-941
      Brucella abortus (strain 9-941): entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3