Q57BH8 (RUVB_BRUAB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Holliday junction ATP-dependent DNA helicase RuvB
EC=3.6.4.12

Gene names

Name:	ruvB
Ordered Locus Names:	BruAb1_1687

Organism

Brucella abortus biovar 1 (strain 9-941) [Complete proteome] [HAMAP]

Taxonomic identifier

262698 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella › Brucella abortus › Brucella abortus bv. 1

Protein attributes

Sequence length	346 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	The RuvA-RuvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is a helicase that mediates the Holliday junction migration by localized denaturation and reannealing By similarity. HAMAP-Rule MF_00016
Catalytic activity	ATP + H2O = ADP + phosphate. HAMAP-Rule MF_00016
Subunit structure	Forms a complex with RuvA By similarity.
Sequence similarities	Belongs to the RuvB family.

Ontologies

Keywords
Biological process	DNA damage DNA recombination DNA repair SOS response
Ligand	ATP-binding Nucleotide-binding
Molecular function	Helicase Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	DNA recombination Inferred from electronic annotation. Source: HAMAP DNA repair Inferred from electronic annotation. Source: HAMAP SOS response Inferred from electronic annotation. Source: HAMAP
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP DNA binding Inferred from electronic annotation. Source: InterPro four-way junction helicase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 346	346	Holliday junction ATP-dependent DNA helicase RuvB HAMAP-Rule MF_00016		PRO_0000235351
Regions
	Nucleotide binding	59 – 66	8	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q57BH8 [UniParc]. Last modified May 10, 2005. Version 1. Checksum: E6671068908197AC	FASTA	346	38,241
	10 20 30 40 50 60 MSDRNPLIDA DRRADEDNTL RPQTLDDFVG QAAARANLKV FIEAAKVRGE ALDHVLFVGP 70 80 90 100 110 120 PGLGKTTLAQ IMAKELGVNF RSTSGPVIAK AGDLAALLTN LEERDVLFID EIHRLSPAVE 130 140 150 160 170 180 EILYPAMEDF QLDLIIGEGP AARSVKIDLA KFTLVAATTR LGLLTTPLRD RFGIPVRLNF 190 200 210 220 230 240 YTVEELEYIV RRGARIMQMG ISSDGAREVA RRSRGTPRIA GRLLRRVRDF ALVAGADIID 250 260 270 280 290 300 RRIADEALSR LEVDNRGLDQ LDRRYLNIIA RNFGGGPVGI ETIAAGLSEP RDAIEDIIEP 310 320 330 340 YLIQQGFLQR TPRGRVLTAV AWQHLGLPAP AEIIQQSQYG LFMEDE

References

[1]

"Completion of the genome sequence of Brucella abortus and comparison to the highly similar genomes of Brucella melitensis and Brucella suis." Halling S.M., Peterson-Burch B.D., Bricker B.J., Zuerner R.L., Qing Z., Li L.-L., Kapur V., Alt D.P., Olsen S.C. J. Bacteriol. 187:2715-2726(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 9-941.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017223 Genomic DNA. Translation: AAX75006.1.
RefSeq	YP_222367.1. NC_006932.1.
3D structure databases
ProteinModelPortal	Q57BH8.
SMR	Q57BH8. Positions 19-330.
ModBase	Search...
Protein-protein interaction databases
STRING	262698.BruAb1_1687.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAX75006; AAX75006; BruAb1_1687.
GeneID	3339647.
KEGG	bmb:BruAb1_1687.
PATRIC	17825158. VBIBruAbo15061_1781.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2255.
HOGENOM	HOG000218623.
KO	K03551.
OMA	HIIAKEM.
ProtClustDB	PRK00080.
Enzyme and pathway databases
BioCyc	BABO262698:GJC2-1727-MONOMER.
Family and domain databases
Gene3D	1.10.10.10. 1 hit.
HAMAP	MF_00016. DNA_helic_RuvB.
InterPro	IPR003593. AAA+_ATPase. IPR000641. CbxX_CfqX. IPR004605. DNA_helicase_Holl-junc_RuvB. IPR008823. DNA_helicase_Holl-junc_RuvB_C. IPR008824. DNA_helicase_Holl-junc_RuvB_N. IPR011991. WHTH_DNA-bd_dom. [Graphical view]
PANTHER	PTHR13779:SF2. PTHR13779:SF2. 1 hit.
Pfam	PF05491. RuvB_C. 1 hit. PF05496. RuvB_N. 1 hit. [Graphical view]
PRINTS	PR00819. CBXCFQXSUPER.
ProDom	PD005323. DNA_helicase_Holl-junc_RuvB_C. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00382. AAA. 1 hit. [Graphical view]
TIGRFAMs	TIGR00635. ruvB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RUVB_BRUAB

Accession

Primary (citable) accession number: Q57BH8

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 16, 2006
Last sequence update:	May 10, 2005
Last modified:	May 1, 2013
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program