Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q57B94 (HYPRE_BRUAB) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 45. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
4-hydroxyproline epimerase

EC=5.1.1.8
Alternative name(s):
Hydroxyproline-2-epimerase
Short name=BaHyPRE
Gene names
Ordered Locus Names:BruAb1_1773
OrganismBrucella abortus biovar 1 (strain 9-941) [Complete proteome] [HAMAP]
Taxonomic identifier262698 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella

Protein attributes

Sequence length333 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Allows intracellular utilization of 4-hydroxyproline, one of the major constituents of host collagen, by converting 4-hydroxy-L-proline to 4-hydroxy-D-proline, which can be further metabolized by intracellular 4-hydroxy-D-proline oxidases. Strong B-cell mitogen. Plays an important role in the regulation of intra- and extracellular amino acid pools, allowing the bacterium to profit from host precursors and enzymatic pathways. Ref.1

Catalytic activity

Trans-4-hydroxy-L-proline = cis-4-hydroxy-D-proline. Ref.1

Enzyme regulation

Inhibited by iodoacetate, iodoacetamide and by high amounts (10mM) of pyrrole-2-carboxylic acid (PYC). Not inhibited by PYC at 1mM. Ref.1

Subunit structure

Homodimer By similarity.

Miscellaneous

This enzyme does not require pyridoxal phosphate (PLP) as a cofactor.

Sequence similarities

Belongs to the proline racemase family. 4-hydroxyproline epimerase subfamily.

Biophysicochemical properties

Kinetic parameters:

KM=9.8 mM for 4-hydroxy-L-proline Ref.1

KM=10.8 mM for 4-hydroxy-D-proline

Vmax=0.40 µM/sec/mg enzyme with L-proline as substrate (at 37 degrees Celsius)

Vmax=0.75 µM/sec/mg enzyme with D-proline as substrate (at 37 degrees Celsius)

Ontologies

Keywords
   Molecular functionIsomerase
   Technical termComplete proteome
Gene Ontology (GO)
   Molecular_function4-hydroxyproline epimerase activity

Inferred from electronic annotation. Source: UniProtKB-EC

proline racemase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 3333334-hydroxyproline epimerase
PRO_0000354027

Sites

Active site901Proton acceptor By similarity
Active site2531Proton donor By similarity

Sequences

Sequence LengthMass (Da)Tools
Q57B94 [UniParc].

Last modified May 10, 2005. Version 1.
Checksum: 34DA2AA5674D5FB9

FASTA33336,670
        10         20         30         40         50         60 
MARHSFFCVD GHTCGNPVRL VAGGGPNLNG STMMEKRAHF LAEYDWIRTG LMFEPRGHDM 

        70         80         90        100        110        120 
MSGSILYPPT RPDCDVAVLF IETSGCLPMC GHGTIGTVTM AIEQGLVTPK TPGKLNLDTP 

       130        140        150        160        170        180 
AGLVAIEYEQ DGQYVERVRL TNVPAFLYAE GLEVECPDLG PIKVDVAYGG NFYAIVEPQE 

       190        200        210        220        230        240 
NYTDMDDYSA LQLIAWSPVL RQRLNEKYKF QHPELPDINR LSHILWTGKP KHPQAHARNA 

       250        260        270        280        290        300 
VFYGDKAIDR SPCGTGTSAR MAQLAAKGKL KPCDEFIHES IIGSLFHGRV ERAAEVAGRP 

       310        320        330 
AIVPSIAGWA RMTGYNTIFI DDRDPFAHGF SMA 

« Hide

References

« Hide 'large scale' references
[1]"Molecular and structural discrimination of proline racemase and hydroxyproline-2-epimerase from nosocomial and bacterial pathogens."
Goytia M., Chamond N., Cosson A., Coatnoan N., Hermant D., Berneman A., Minoprio P.
PLoS ONE 2:E885-E885(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CATALYTIC ACTIVITY, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES, ROLE IN VIRULENCE.
Strain: 544 / Biovar 1.
[2]"Completion of the genome sequence of Brucella abortus and comparison to the highly similar genomes of Brucella melitensis and Brucella suis."
Halling S.M., Peterson-Burch B.D., Bricker B.J., Zuerner R.L., Qing Z., Li L.-L., Kapur V., Alt D.P., Olsen S.C.
J. Bacteriol. 187:2715-2726(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 9-941.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
EF495344 Genomic DNA. Translation: ABS82396.1.
AE017223 Genomic DNA. Translation: AAX75090.1.
RefSeqYP_222451.1. NC_006932.1.

3D structure databases

ProteinModelPortalQ57B94.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING262698.BruAb1_1773.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAX75090; AAX75090; BruAb1_1773.
GeneID3340991.
KEGGbmb:BruAb1_1773.
PATRIC17825335. VBIBruAbo15061_1869.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG3938.
HOGENOMHOG000084336.
KOK12658.
OMAFGGNFYA.
OrthoDBEOG6SNDV3.
ProtClustDBPRK13971.

Enzyme and pathway databases

BioCycBABO262698:GJC2-1813-MONOMER.
BRENDA5.1.1.8. 994.

Family and domain databases

InterProIPR008794. Pro_racemase_fam.
[Graphical view]
PfamPF05544. Pro_racemase. 1 hit.
[Graphical view]
PIRSFPIRSF029792. Pro_racemase. 1 hit.
ProtoNetSearch...

Entry information

Entry nameHYPRE_BRUAB
AccessionPrimary (citable) accession number: Q57B94
Secondary accession number(s): A8DEY8
Entry history
Integrated into UniProtKB/Swiss-Prot: November 25, 2008
Last sequence update: May 10, 2005
Last modified: April 16, 2014
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Brucella abortus strain 9-941

Brucella abortus (strain 9-941): entries and gene names