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Q57B94

- HYPRE_BRUAB

UniProt

Q57B94 - HYPRE_BRUAB

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Protein

4-hydroxyproline epimerase

Gene
BruAb1_1773
Organism
Brucella abortus biovar 1 (strain 9-941)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Allows intracellular utilization of 4-hydroxyproline, one of the major constituents of host collagen, by converting 4-hydroxy-L-proline to 4-hydroxy-D-proline, which can be further metabolized by intracellular 4-hydroxy-D-proline oxidases. Strong B-cell mitogen. Plays an important role in the regulation of intra- and extracellular amino acid pools, allowing the bacterium to profit from host precursors and enzymatic pathways.1 Publication

Catalytic activityi

Trans-4-hydroxy-L-proline = cis-4-hydroxy-D-proline.1 Publication

Enzyme regulationi

Inhibited by iodoacetate, iodoacetamide and by high amounts (10mM) of pyrrole-2-carboxylic acid (PYC). Not inhibited by PYC at 1mM.1 Publication

Kineticsi

  1. KM=9.8 mM for 4-hydroxy-L-proline1 Publication
  2. KM=10.8 mM for 4-hydroxy-D-proline

Vmax=0.40 µM/sec/mg enzyme with L-proline as substrate (at 37 degrees Celsius)

Vmax=0.75 µM/sec/mg enzyme with D-proline as substrate (at 37 degrees Celsius)

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei90 – 901Proton acceptor By similarity
Active sitei253 – 2531Proton donor By similarity

GO - Molecular functioni

  1. 4-hydroxyproline epimerase activity Source: UniProtKB-EC
  2. proline racemase activity Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Enzyme and pathway databases

BioCyciBABO262698:GJC2-1813-MONOMER.
BRENDAi5.1.1.8. 994.

Names & Taxonomyi

Protein namesi
Recommended name:
4-hydroxyproline epimerase (EC:5.1.1.8)
Alternative name(s):
Hydroxyproline-2-epimerase
Short name:
BaHyPRE
Gene namesi
Ordered Locus Names:BruAb1_1773
OrganismiBrucella abortus biovar 1 (strain 9-941)
Taxonomic identifieri262698 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
ProteomesiUP000000540: Chromosome I

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 3333334-hydroxyproline epimerasePRO_0000354027Add
BLAST

Interactioni

Subunit structurei

Homodimer By similarity.

Protein-protein interaction databases

STRINGi262698.BruAb1_1773.

Structurei

3D structure databases

ProteinModelPortaliQ57B94.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG3938.
HOGENOMiHOG000084336.
KOiK12658.
OMAiKNFRDMA.
OrthoDBiEOG6SNDV3.

Family and domain databases

InterProiIPR008794. Pro_racemase_fam.
[Graphical view]
PfamiPF05544. Pro_racemase. 1 hit.
[Graphical view]
PIRSFiPIRSF029792. Pro_racemase. 1 hit.

Sequencei

Sequence statusi: Complete.

Q57B94-1 [UniParc]FASTAAdd to Basket

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MARHSFFCVD GHTCGNPVRL VAGGGPNLNG STMMEKRAHF LAEYDWIRTG    50
LMFEPRGHDM MSGSILYPPT RPDCDVAVLF IETSGCLPMC GHGTIGTVTM 100
AIEQGLVTPK TPGKLNLDTP AGLVAIEYEQ DGQYVERVRL TNVPAFLYAE 150
GLEVECPDLG PIKVDVAYGG NFYAIVEPQE NYTDMDDYSA LQLIAWSPVL 200
RQRLNEKYKF QHPELPDINR LSHILWTGKP KHPQAHARNA VFYGDKAIDR 250
SPCGTGTSAR MAQLAAKGKL KPCDEFIHES IIGSLFHGRV ERAAEVAGRP 300
AIVPSIAGWA RMTGYNTIFI DDRDPFAHGF SMA 333
Length:333
Mass (Da):36,670
Last modified:May 10, 2005 - v1
Checksum:i34DA2AA5674D5FB9
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
EF495344 Genomic DNA. Translation: ABS82396.1.
AE017223 Genomic DNA. Translation: AAX75090.1.
RefSeqiYP_222451.1. NC_006932.1.

Genome annotation databases

EnsemblBacteriaiAAX75090; AAX75090; BruAb1_1773.
GeneIDi3340991.
KEGGibmb:BruAb1_1773.
PATRICi17825335. VBIBruAbo15061_1869.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
EF495344 Genomic DNA. Translation: ABS82396.1 .
AE017223 Genomic DNA. Translation: AAX75090.1 .
RefSeqi YP_222451.1. NC_006932.1.

3D structure databases

ProteinModelPortali Q57B94.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 262698.BruAb1_1773.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAX75090 ; AAX75090 ; BruAb1_1773 .
GeneIDi 3340991.
KEGGi bmb:BruAb1_1773.
PATRICi 17825335. VBIBruAbo15061_1869.

Phylogenomic databases

eggNOGi COG3938.
HOGENOMi HOG000084336.
KOi K12658.
OMAi KNFRDMA.
OrthoDBi EOG6SNDV3.

Enzyme and pathway databases

BioCyci BABO262698:GJC2-1813-MONOMER.
BRENDAi 5.1.1.8. 994.

Family and domain databases

InterProi IPR008794. Pro_racemase_fam.
[Graphical view ]
Pfami PF05544. Pro_racemase. 1 hit.
[Graphical view ]
PIRSFi PIRSF029792. Pro_racemase. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular and structural discrimination of proline racemase and hydroxyproline-2-epimerase from nosocomial and bacterial pathogens."
    Goytia M., Chamond N., Cosson A., Coatnoan N., Hermant D., Berneman A., Minoprio P.
    PLoS ONE 2:E885-E885(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CATALYTIC ACTIVITY, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES, ROLE IN VIRULENCE.
    Strain: 544 / Biovar 1.
  2. "Completion of the genome sequence of Brucella abortus and comparison to the highly similar genomes of Brucella melitensis and Brucella suis."
    Halling S.M., Peterson-Burch B.D., Bricker B.J., Zuerner R.L., Qing Z., Li L.-L., Kapur V., Alt D.P., Olsen S.C.
    J. Bacteriol. 187:2715-2726(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 9-941.

Entry informationi

Entry nameiHYPRE_BRUAB
AccessioniPrimary (citable) accession number: Q57B94
Secondary accession number(s): A8DEY8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 25, 2008
Last sequence update: May 10, 2005
Last modified: May 14, 2014
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

This enzyme does not require pyridoxal phosphate (PLP) as a cofactor.

Keywords - Technical termi

Complete proteome

Documents

  1. Brucella abortus strain 9-941
    Brucella abortus (strain 9-941): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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