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Q57B94

- HYPRE_BRUAB

UniProt

Q57B94 - HYPRE_BRUAB

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Protein
4-hydroxyproline epimerase
Gene
BruAb1_1773
Organism
Brucella abortus biovar 1 (strain 9-941)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Allows intracellular utilization of 4-hydroxyproline, one of the major constituents of host collagen, by converting 4-hydroxy-L-proline to 4-hydroxy-D-proline, which can be further metabolized by intracellular 4-hydroxy-D-proline oxidases. Strong B-cell mitogen. Plays an important role in the regulation of intra- and extracellular amino acid pools, allowing the bacterium to profit from host precursors and enzymatic pathways.1 Publication

Catalytic activityi

Trans-4-hydroxy-L-proline = cis-4-hydroxy-D-proline.1 Publication

Enzyme regulationi

Inhibited by iodoacetate, iodoacetamide and by high amounts (10mM) of pyrrole-2-carboxylic acid (PYC). Not inhibited by PYC at 1mM.1 Publication

Kineticsi

  1. KM=9.8 mM for 4-hydroxy-L-proline1 Publication
  2. KM=10.8 mM for 4-hydroxy-D-proline

Vmax=0.40 µM/sec/mg enzyme with L-proline as substrate (at 37 degrees Celsius)

Vmax=0.75 µM/sec/mg enzyme with D-proline as substrate (at 37 degrees Celsius)

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei90 – 901Proton acceptor By similarity
Active sitei253 – 2531Proton donor By similarity

GO - Molecular functioni

  1. 4-hydroxyproline epimerase activity Source: UniProtKB-EC
  2. proline racemase activity Source: InterPro
Complete GO annotation...

GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Isomerase

    Enzyme and pathway databases

    BioCyciBABO262698:GJC2-1813-MONOMER.
    BRENDAi5.1.1.8. 994.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    4-hydroxyproline epimerase (EC:5.1.1.8)
    Alternative name(s):
    Hydroxyproline-2-epimerase
    Short name:
    BaHyPRE
    Gene namesi
    Ordered Locus Names:BruAb1_1773
    OrganismiBrucella abortus biovar 1 (strain 9-941)
    Taxonomic identifieri262698 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
    ProteomesiUP000000540: Chromosome I

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 3333334-hydroxyproline epimerase
    PRO_0000354027Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer By similarity.

    Protein-protein interaction databases

    STRINGi262698.BruAb1_1773.

    Structurei

    3D structure databases

    ProteinModelPortaliQ57B94.

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG3938.
    HOGENOMiHOG000084336.
    KOiK12658.
    OMAiKNFRDMA.
    OrthoDBiEOG6SNDV3.

    Family and domain databases

    InterProiIPR008794. Pro_racemase_fam.
    [Graphical view]
    PfamiPF05544. Pro_racemase. 1 hit.
    [Graphical view]
    PIRSFiPIRSF029792. Pro_racemase. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q57B94-1 [UniParc]FASTAAdd to Basket

    « Hide

    MARHSFFCVD GHTCGNPVRL VAGGGPNLNG STMMEKRAHF LAEYDWIRTG    50
    LMFEPRGHDM MSGSILYPPT RPDCDVAVLF IETSGCLPMC GHGTIGTVTM 100
    AIEQGLVTPK TPGKLNLDTP AGLVAIEYEQ DGQYVERVRL TNVPAFLYAE 150
    GLEVECPDLG PIKVDVAYGG NFYAIVEPQE NYTDMDDYSA LQLIAWSPVL 200
    RQRLNEKYKF QHPELPDINR LSHILWTGKP KHPQAHARNA VFYGDKAIDR 250
    SPCGTGTSAR MAQLAAKGKL KPCDEFIHES IIGSLFHGRV ERAAEVAGRP 300
    AIVPSIAGWA RMTGYNTIFI DDRDPFAHGF SMA 333
    Length:333
    Mass (Da):36,670
    Last modified:May 10, 2005 - v1
    Checksum:i34DA2AA5674D5FB9
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    EF495344 Genomic DNA. Translation: ABS82396.1.
    AE017223 Genomic DNA. Translation: AAX75090.1.
    RefSeqiYP_222451.1. NC_006932.1.

    Genome annotation databases

    EnsemblBacteriaiAAX75090; AAX75090; BruAb1_1773.
    GeneIDi3340991.
    KEGGibmb:BruAb1_1773.
    PATRICi17825335. VBIBruAbo15061_1869.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    EF495344 Genomic DNA. Translation: ABS82396.1 .
    AE017223 Genomic DNA. Translation: AAX75090.1 .
    RefSeqi YP_222451.1. NC_006932.1.

    3D structure databases

    ProteinModelPortali Q57B94.
    ModBasei Search...

    Protein-protein interaction databases

    STRINGi 262698.BruAb1_1773.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAX75090 ; AAX75090 ; BruAb1_1773 .
    GeneIDi 3340991.
    KEGGi bmb:BruAb1_1773.
    PATRICi 17825335. VBIBruAbo15061_1869.

    Phylogenomic databases

    eggNOGi COG3938.
    HOGENOMi HOG000084336.
    KOi K12658.
    OMAi KNFRDMA.
    OrthoDBi EOG6SNDV3.

    Enzyme and pathway databases

    BioCyci BABO262698:GJC2-1813-MONOMER.
    BRENDAi 5.1.1.8. 994.

    Family and domain databases

    InterProi IPR008794. Pro_racemase_fam.
    [Graphical view ]
    Pfami PF05544. Pro_racemase. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF029792. Pro_racemase. 1 hit.
    ProtoNeti Search...

    Publicationsi

    « Hide 'large scale' publications
    1. "Molecular and structural discrimination of proline racemase and hydroxyproline-2-epimerase from nosocomial and bacterial pathogens."
      Goytia M., Chamond N., Cosson A., Coatnoan N., Hermant D., Berneman A., Minoprio P.
      PLoS ONE 2:E885-E885(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CATALYTIC ACTIVITY, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES, ROLE IN VIRULENCE.
      Strain: 544 / Biovar 1.
    2. "Completion of the genome sequence of Brucella abortus and comparison to the highly similar genomes of Brucella melitensis and Brucella suis."
      Halling S.M., Peterson-Burch B.D., Bricker B.J., Zuerner R.L., Qing Z., Li L.-L., Kapur V., Alt D.P., Olsen S.C.
      J. Bacteriol. 187:2715-2726(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 9-941.

    Entry informationi

    Entry nameiHYPRE_BRUAB
    AccessioniPrimary (citable) accession number: Q57B94
    Secondary accession number(s): A8DEY8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 25, 2008
    Last sequence update: May 10, 2005
    Last modified: May 14, 2014
    This is version 46 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    This enzyme does not require pyridoxal phosphate (PLP) as a cofactor.

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Brucella abortus strain 9-941
      Brucella abortus (strain 9-941): entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

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