Reviewed, UniProtKB/Swiss-Prot Q57B76 (ACSA_BRUAB)

Names and origin

Protein names

Recommended name:
    Acetyl-coenzyme A synthetase
    EC=6.2.1.1
Alternative name(s):
    Acetate--CoA ligase
    Acyl-activating enzyme

Gene names

Name:	acsA
Ordered Locus Names:	BruAb1_1791

Organism

Brucella abortus [Complete proteome] [HAMAP]

Taxonomic identifier

235 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	651 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA. HAMAP MF_01123
Post-translational modification	Acetylated. Deacetylation by the SIR2-homolog deacetylase activates the enzyme By similarity.
Sequence similarities	Belongs to the ATP-dependent AMP-binding enzyme family.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
PTM	Acetylation
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	AMP binding Inferred from electronic annotation. Source: InterPro ATP binding Inferred from electronic annotation. Source: UniProtKB-KW acetate-CoA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 651	651	Acetyl-coenzyme A synthetase HAMAP MF_01123		PRO_1000065275
Sites
	Active site	517	1	By similarity
Amino acid modifications
	Modified residue	611	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q57B76-1 [UniParc]. Last modified May 10, 2005. Version 1. Checksum: 7FB222A3EE6476CE	FASTA	651	72,731
	10 20 30 40 50 60 MSEKLYPVLP EAKKNTLIDN ETYLEWYEES VSDPDGFWAK HGRRIDWFKP FTKVKNTDFN 70 80 90 100 110 120 GDVTIKWYED GVTNVSYNCI DRHLKSRGDK VAIIWEGDNP YIDKKITYRE LYENVCRMAN 130 140 150 160 170 180 VLKKHGVKKG DRVTIYLPMI PEAAYAMLAC ARIGAVHSVV FAGFSPEALA GRIVDCESTF 190 200 210 220 230 240 VITADEGVRG GKPVALKENT DTAIDIAAKQ YVMVNKVLVV RRTGGKVSWG RGRDLWYHQE 250 260 270 280 290 300 VASVEPHCEP EPMNAEDPLF ILYTSGSTGK PKGVLHTTGG YLVYASMTHQ YVFDYHDGEI 310 320 330 340 350 360 YWCTADVGWV TGHSYIVYGP LANGATTLMF EGVPNFPDQG RFWEVVDKHH VNIFYTAPTA 370 380 390 400 410 420 LRALMGAGDE FVTRSSRSTL RLLGSVGEPI NPEAWEWYYN VVGDQKCPIV DTWWQTENGG 430 440 450 460 470 480 ILITPLPGAT DLKPGSATRP FFGVKPVLVD NEGNVQEGVA DGNLCISDSW PGQMRTVYGD 490 500 510 520 530 540 HKRFIETYFS TYKGMYFSGD GCRRDEDGYY WITGRVDDVL NISGHRLGTA EIESALVSHH 550 560 570 580 590 600 SVSEAAVVGY PHPIKGQGIY CYVTLMTGAD AQDPDELRKE LVQHVRKEIG PIATPDKIQF 610 620 630 640 650 APGLPKTRSG KIMRRILRKI AEDEFGALGD TSTLADPGVV DDLIENRQNK K

References

[1]

"Completion of the genome sequence of Brucella abortus and comparison to the highly similar genomes of Brucella melitensis and Brucella suis." Halling S.M., Peterson-Burch B.D., Bricker B.J., Zuerner R.L., Qing Z., Li L.-L., Kapur V., Alt D.P., Olsen S.C. J. Bacteriol. 187:2715-2726(2005) [PubMed: 15805518] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 9-941 / Biovar 1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE017223 Genomic DNA. Translation: AAX75108.1.
RefSeq	YP_222469.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3340723.
GenomeReviews	Gene locus BruAb1_1791 in contig AE017223_GR.
KEGG	bmb:BruAb1_1791.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q57B76.
OMA	ETASEHC.
Enzyme and pathway databases
BioCyc	BABO262698:BRUAB1_1791-MON.
BRENDA	6.2.1.1. 575.
Family and domain databases
HAMAP	MF_01123. [Tree]
InterPro	IPR011904. Ac_CoA_lig_AcsA. IPR000873. AMP-dep_Synth/Lig. [Graphical view]
Pfam	PF00501. AMP-binding. 1 hit. [Graphical view]
TIGRFAMs	TIGR02188. Ac_CoA_lig_AcsA. 1 hit.
PROSITE	PS00455. AMP_BINDING. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ACSA_BRUAB

Accession

Primary (citable) accession number: Q57B76

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	May 10, 2005
Last modified:	November 3, 2009
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)