Q57AJ8 (RSMG_BRUAB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribosomal RNA small subunit methyltransferase G
EC=2.1.1.170

Alternative name(s):
16S rRNA 7-methylguanosine methyltransferase
Short name=16S rRNA m7G methyltransferase

Gene names

Name:	rsmG
Ordered Locus Names:	BruAb1_2035

Organism

Brucella abortus biovar 1 (strain 9-941) [Complete proteome] [HAMAP]

Taxonomic identifier

262698 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella › Brucella abortus › Brucella abortus bv. 1

Protein attributes

Sequence length	213 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Specifically methylates the N7 position of guanine in position 527 of 16S rRNA By similarity. HAMAP-Rule MF_00074
Catalytic activity	S-adenosyl-L-methionine + guanine(527) in 16S rRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(527) in 16S rRNA. HAMAP-Rule MF_00074
Subcellular location	Cytoplasm Potential HAMAP-Rule MF_00074.
Sequence similarities	Belongs to the methyltransferase superfamily. RNA methyltransferase RsmG family.

Ontologies

Keywords
Biological process	rRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	rRNA (guanine-N7)-methylation Inferred from electronic annotation. Source: GOC
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	rRNA (guanine-N7-)-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 213	213	Ribosomal RNA small subunit methyltransferase G HAMAP-Rule MF_00074		PRO_0000184228
Regions
	Region	128 – 129	2	S-adenosyl-L-methionine binding By similarity
Sites
	Binding site	75	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity
	Binding site	80	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity
	Binding site	144	1	S-adenosyl-L-methionine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q57AJ8 [UniParc]. Last modified May 10, 2005. Version 1. Checksum: D9B5B1C9467AB2E2	FASTA	213	23,473
	10 20 30 40 50 60 MSADIRFDSL KTIVPAVSRE TADRLIAFED LFRKWSKAIN LASPSTLADL WNRHILDSAQ 70 80 90 100 110 120 LFPLAKEATR WLDIGSGGGF PGIVTACFLA ERSGGCIDLV ESAGKKAAFL RTAAGHLHVP 130 140 150 160 170 180 ARVHSARIES MWEKIETPQV VTARALASLG DLFTLAEPWL SDGAKALFQK GRDYQREIDE 190 200 210 SRVGWSFDLV KHPSAIDQAS VILEISNLRR KTD

References

[1]

"Completion of the genome sequence of Brucella abortus and comparison to the highly similar genomes of Brucella melitensis and Brucella suis." Halling S.M., Peterson-Burch B.D., Bricker B.J., Zuerner R.L., Qing Z., Li L.-L., Kapur V., Alt D.P., Olsen S.C. J. Bacteriol. 187:2715-2726(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 9-941.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017223 Genomic DNA. Translation: AAX75336.1.
RefSeq	YP_222697.1. NC_006932.1.
3D structure databases
ProteinModelPortal	Q57AJ8.
ModBase	Search...
Protein-protein interaction databases
STRING	262698.BruAb1_2035.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAX75336; AAX75336; BruAb1_2035.
GeneID	3339666.
KEGG	bmb:BruAb1_2035.
PATRIC	17825917. VBIBruAbo15061_2146.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0357.
HOGENOM	HOG000221015.
KO	K03501.
OMA	FHKGRDY.
ProtClustDB	PRK00107.
Enzyme and pathway databases
BioCyc	BABO262698:GJC2-2085-MONOMER.
Family and domain databases
HAMAP	MF_00074. 16SrRNA_methyltr_G.
InterPro	IPR003682. rRNA_ssu_MeTfrase_G. [Graphical view]
Pfam	PF02527. GidB. 1 hit. [Graphical view]
PIRSF	PIRSF003078. GidB. 1 hit.
TIGRFAMs	TIGR00138. gidB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RSMG_BRUAB

Accession

Primary (citable) accession number: Q57AJ8

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 22, 2005
Last sequence update:	May 10, 2005
Last modified:	May 1, 2013
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program