Q57AH5 (HIS5_BRUAB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Imidazole glycerol phosphate synthase subunit HisH
EC=2.4.2.-

Alternative name(s):
IGP synthase glutamine amidotransferase subunit
IGP synthase subunit HisH
ImGP synthase subunit HisH
Short name=IGPS subunit HisH

Gene names

Name:	hisH
Ordered Locus Names:	BruAb1_2058

Organism

Brucella abortus biovar 1 (strain 9-941) [Complete proteome] [HAMAP]

Taxonomic identifier

262698 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella › Brucella abortus › Brucella abortus bv. 1

Protein attributes

Sequence length	216 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The HisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to HisF for the synthesis of IGP and AICAR By similarity. HAMAP-Rule MF_00278
Catalytic activity	5-[(5-phospho-1-deoxyribulos-1-ylamino)methylideneamino]-1-(5-phosphoribosyl)imidazole-4-carboxamide + L-glutamine = imidazole-glycerol phosphate + 5-aminoimidazol-4-carboxamide ribonucleotide + L-glutamate + H2O. HAMAP-Rule MF_00278
Pathway	Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 5/9. HAMAP-Rule MF_00278
Subunit structure	Heterodimer of HisH and HisF By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Histidine biosynthesis
Cellular component	Cytoplasm
Domain	Glutamine amidotransferase
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	glutamine metabolic process Inferred from electronic annotation. Source: HAMAP histidine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	imidazoleglycerol-phosphate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 216	216	Imidazole glycerol phosphate synthase subunit HisH HAMAP-Rule MF_00278		PRO_0000231707
Regions
	Domain	2 – 216	215	Glutamine amidotransferase type-1
Sites
	Active site	88	1	Nucleophile By similarity
	Active site	196	1	By similarity
	Active site	198	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q57AH5 [UniParc]. Last modified May 10, 2005. Version 1. Checksum: E87CAEF18E2D1A09	FASTA	216	23,232
	10 20 30 40 50 60 MRVAIIDYGS GNLRSATKAF ERAAHESGIS AEIDLTCDAQ RVASADRIVL PGVGAYADCR 70 80 90 100 110 120 RGLDAAPGMV EALNDTVLKK ARPFLGICVG MQLMSERGLE KTVTNGLGWI AGDVREMVPS 130 140 150 160 170 180 DASLKIPQIG WNRIHVKHSH PIFDGIPTGD DGLHAYFVHS YMLDAKNASD VLAVTDYGGD 190 200 210 VTAAVGRDNM VGTQFHPEKS QLLGLSLIAN FLKWKP

References

[1]

"Completion of the genome sequence of Brucella abortus and comparison to the highly similar genomes of Brucella melitensis and Brucella suis." Halling S.M., Peterson-Burch B.D., Bricker B.J., Zuerner R.L., Qing Z., Li L.-L., Kapur V., Alt D.P., Olsen S.C. J. Bacteriol. 187:2715-2726(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 9-941.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017223 Genomic DNA. Translation: AAX75359.1.
RefSeq	YP_222720.1. NC_006932.1.
3D structure databases
ProteinModelPortal	Q57AH5.
ModBase	Search...
Protein-protein interaction databases
STRING	262698.BruAb1_2058.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAX75359; AAX75359; BruAb1_2058.
GeneID	3339689.
KEGG	bmb:BruAb1_2058.
PATRIC	17825969. VBIBruAbo15061_2172.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0118.
HOGENOM	HOG000025030.
KO	K02501.
OMA	VHSYHLA.
ProtClustDB	PRK13146.
Enzyme and pathway databases
BioCyc	BABO262698:GJC2-2108-MONOMER.
UniPathway	UPA00031; UER00010.
Family and domain databases
HAMAP	MF_00278. HisH.
InterPro	IPR017926. GATASE_1. IPR010139. Imidazole-glycPsynth_HisH. [Graphical view]
Pfam	PF00117. GATase. 1 hit. [Graphical view]
PIRSF	PIRSF000495. Amidotransf_hisH. 1 hit.
TIGRFAMs	TIGR01855. IMP_synth_hisH. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HIS5_BRUAB

Accession

Primary (citable) accession number: Q57AH5

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 4, 2006
Last sequence update:	May 10, 2005
Last modified:	May 1, 2013
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program