Q57A93 (FABA_BRUAB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase
EC=4.2.1.59

Alternative name(s):
3-hydroxyacyl-[acyl-carrier-protein] dehydratase FabA
Beta-hydroxydecanoyl thioester dehydrase
Trans-2-decenoyl-[acyl-carrier-protein] isomerase
EC=5.3.3.14

Gene names

Name:	fabA
Ordered Locus Names:	BruAb1_2146

Organism

Brucella abortus biovar 1 (strain 9-941) [Complete proteome] [HAMAP]

Taxonomic identifier

262698 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella › Brucella abortus › Brucella abortus bv. 1

Protein attributes

Sequence length	172 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Necessary for the introduction of cis unsaturation into fatty acids. Catalyzes the dehydration of (3R)-3-hydroxydecanoyl-ACP to E-(2)-decenoyl-ACP and then its isomerization to Z-(3)-decenoyl-ACP. Can catalyze the dehydratase reaction for beta-hydroxyacyl-ACPs with saturated chain lengths up to 16:0, being most active on intermediate chain length By similarity. HAMAP-Rule MF_00405
Catalytic activity	A (3R)-3-hydroxyacyl-[acyl-carrier protein] = a trans-2-enoyl-[acyl-carrier protein] + H2O. HAMAP-Rule MF_00405 (3R)-3-hydroxydecanoyl-[acyl-carrier-protein] = trans-dec-2-enoyl-[acyl-carrier-protein] + H2O. HAMAP-Rule MF_00405 Trans-dec-2-enoyl-[acyl-carrier-protein] = cis-dec-3-enoyl-[acyl-carrier-protein]. HAMAP-Rule MF_00405
Pathway	Lipid metabolism; fatty acid biosynthesis. HAMAP-Rule MF_00405
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the thioester dehydratase family. FabA subfamily.

Ontologies

Keywords
Biological process	Fatty acid biosynthesis Fatty acid metabolism Lipid biosynthesis Lipid metabolism
Cellular component	Cytoplasm
Molecular function	Isomerase Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	fatty acid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase activity Inferred from electronic annotation. Source: HAMAP 3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase activity Inferred from electronic annotation. Source: EC trans-2-decenoyl-acyl-carrier-protein isomerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 172	172	3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase HAMAP-Rule MF_00405		PRO_0000267723
Sites
	Active site	71	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q57A93 [UniParc]. Last modified May 10, 2005. Version 1. Checksum: 492701CC908DB72A	FASTA	172	19,086
	10 20 30 40 50 60 MAEQKSSYGY EELLACGRGE MFGPGNAQLP LPPMLMIHRI TEISETGGAF DKGYIRAEYD 70 80 90 100 110 120 VRPDDWYFPC HFQGNPIMPG CLGLDGMWQL TGFFLGWLGE PGRGMALSTG EVKFKGMVRP 130 140 150 160 170 HTKLLEYGID FKRVMRGRLV LGTADGWLKA DGELIYQATD LRVGLSKEGS AQ

References

[1]

"Completion of the genome sequence of Brucella abortus and comparison to the highly similar genomes of Brucella melitensis and Brucella suis." Halling S.M., Peterson-Burch B.D., Bricker B.J., Zuerner R.L., Qing Z., Li L.-L., Kapur V., Alt D.P., Olsen S.C. J. Bacteriol. 187:2715-2726(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 9-941.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017223 Genomic DNA. Translation: AAX75441.1.
RefSeq	YP_222802.1. NC_006932.1.
3D structure databases
HSSP	HSSP built from PDB template 1MKA based on UniProtKB P0A6Q3.
ProteinModelPortal	Q57A93.
SMR	Q57A93. Positions 3-171.
ModBase	Search...
Protein-protein interaction databases
STRING	262698.BruAb1_2146.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAX75441; AAX75441; BruAb1_2146.
GeneID	3340646.
KEGG	bmb:BruAb1_2146.
PATRIC	17826159. VBIBruAbo15061_2267.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0764.
HOGENOM	HOG000277828.
KO	K01716.
OMA	KGRAIST.
ProtClustDB	PRK05174.
Enzyme and pathway databases
BioCyc	BABO262698:GJC2-2196-MONOMER.
UniPathway	UPA00094.
Family and domain databases
HAMAP	MF_00405. FabA.
InterPro	IPR010083. FabA. IPR013114. FabA_FabZ. [Graphical view]
Pfam	PF07977. FabA. 1 hit. [Graphical view]
TIGRFAMs	TIGR01749. fabA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

FABA_BRUAB

Accession

Primary (citable) accession number: Q57A93

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 12, 2006
Last sequence update:	May 10, 2005
Last modified:	May 1, 2013
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program